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. 1993 Mar;57(1):1–33. doi: 10.1128/mr.57.1.1-33.1993

Bacillus subtilis sporulation: regulation of gene expression and control of morphogenesis.

J Errington 1
PMCID: PMC372899  PMID: 8464402

Abstract

Bacillus subtilis sporulation is an adaptive response to nutritional stress and involves the differential development of two cells. In the last 10 years or so, virtually all of the regulatory genes controlling sporulation, and many genes directing the structural and morphological changes that accompany sporulation, have been cloned and characterized. This review describes our current knowledge of the program of gene expression during sporulation and summarizes what is known about the functions of the genes that determine the specialized biochemical and morphological properties of sporulating cells. Most steps in the genetic program are controlled by transcription factors that have been characterized in vitro. Two sporulation-specific sigma factors, sigma E and sigma F, appear to segregate at septation, effectively determining the differential development of the mother cell and prespore. Later, each sigma is replaced by a second cell-specific sigma factor, sigma K in the mother cell and sigma G in the prespore. The synthesis of each sigma factor is tightly regulated at both the transcriptional and posttranslational levels. Usually this regulation involves an intercellular interaction that coordinates the developmental programmes of the two cells. At least two other transcription factors fine tune the timing and levels of expression of genes in the sigma E and sigma K regulons. The controlled synthesis of the sigma factors and other transcription factors leads to a spatially and temporally ordered program of gene expression. The gene products made during each successive stage of sporulation help to bring about a sequence of gross morphological changes and biochemical adaptations. The formation of the asymmetric spore septum, engulfment of the prespore by the mother cell, and formation of the spore core, cortex, and coat are described. The importance of these structures in the development of the resistance, dormancy, and germination properties of the spore is assessed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adams L. F., Brown K. L., Whiteley H. R. Molecular cloning and characterization of two genes encoding sigma factors that direct transcription from a Bacillus thuringiensis crystal protein gene promoter. J Bacteriol. 1991 Jun;173(12):3846–3854. doi: 10.1128/jb.173.12.3846-3854.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Adler H. I., Fisher W. D., Cohen A., Hardigree A. A. MINIATURE escherichia coli CELLS DEFICIENT IN DNA. Proc Natl Acad Sci U S A. 1967 Feb;57(2):321–326. doi: 10.1073/pnas.57.2.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Akrigg A., Mandelstam J. Extracellular manganese-stimulated deoxyribonuclease as a marker event in sporulation of Bacillus subtilis. Biochem J. 1978 Apr 15;172(1):63–67. doi: 10.1042/bj1720063. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Akrigg A. Purification and properties of a manganese-stimulated deoxyribonuclease produced during sporulation of Bacillus subtilis. Biochem J. 1978 Apr 15;172(1):69–76. doi: 10.1042/bj1720069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Andreoli A. J., Suehiro S., Sakiyama D., Takemoto J., Vivanco E., Lara J. C., Klute M. C. Release and recovery of forespores from Bacillus cereus. J Bacteriol. 1973 Sep;115(3):1159–1166. doi: 10.1128/jb.115.3.1159-1166.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Antoniewski C., Savelli B., Stragier P. The spoIIJ gene, which regulates early developmental steps in Bacillus subtilis, belongs to a class of environmentally responsive genes. J Bacteriol. 1990 Jan;172(1):86–93. doi: 10.1128/jb.172.1.86-93.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Aronson A. I., Song H. Y., Bourne N. Gene structure and precursor processing of a novel Bacillus subtilis spore coat protein. Mol Microbiol. 1989 Mar;3(3):437–444. doi: 10.1111/j.1365-2958.1989.tb00189.x. [DOI] [PubMed] [Google Scholar]
  8. Bach M. L., Gilvarg C. Biosynthesis of dipicolinic acid in sporulating Bacillus megaterium. J Biol Chem. 1966 Oct 10;241(19):4563–4564. [PubMed] [Google Scholar]
  9. Balassa G., Milhaud P., Raulet E., Silva M. T., Sousa J. C. A Bacillus subtilis mutant requiring dipicolinic acid for the development of heat-resistant spores. J Gen Microbiol. 1979 Feb;110(2):365–379. doi: 10.1099/00221287-110-2-365. [DOI] [PubMed] [Google Scholar]
  10. Beall B., Lowe M., Lutkenhaus J. Cloning and characterization of Bacillus subtilis homologs of Escherichia coli cell division genes ftsZ and ftsA. J Bacteriol. 1988 Oct;170(10):4855–4864. doi: 10.1128/jb.170.10.4855-4864.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Beall B., Lutkenhaus J. FtsZ in Bacillus subtilis is required for vegetative septation and for asymmetric septation during sporulation. Genes Dev. 1991 Mar;5(3):447–455. doi: 10.1101/gad.5.3.447. [DOI] [PubMed] [Google Scholar]
  12. Beall B., Lutkenhaus J. Nucleotide sequence and insertional inactivation of a Bacillus subtilis gene that affects cell division, sporulation, and temperature sensitivity. J Bacteriol. 1989 Dec;171(12):6821–6834. doi: 10.1128/jb.171.12.6821-6834.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  14. Bi E., Lutkenhaus J. FtsZ regulates frequency of cell division in Escherichia coli. J Bacteriol. 1990 May;172(5):2765–2768. doi: 10.1128/jb.172.5.2765-2768.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Bookstein C., Edwards C. W., Kapp N. V., Hulett F. M. The Bacillus subtilis 168 alkaline phosphatase III gene: impact of a phoAIII mutation on total alkaline phosphatase synthesis. J Bacteriol. 1990 Jul;172(7):3730–3737. doi: 10.1128/jb.172.7.3730-3737.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Bourne N., FitzJames P. C., Aronson A. I. Structural and germination defects of Bacillus subtilis spores with altered contents of a spore coat protein. J Bacteriol. 1991 Oct;173(20):6618–6625. doi: 10.1128/jb.173.20.6618-6625.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Bouvier J., Stragier P., Bonamy C., Szulmajster J. Nucleotide sequence of the spo0B gene of Bacillus subtilis and regulation of its expression. Proc Natl Acad Sci U S A. 1984 Nov;81(22):7012–7016. doi: 10.1073/pnas.81.22.7012. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Buchanan C. E., Gustafson A. Mutagenesis and mapping of the gene for a sporulation-specific penicillin-binding protein in Bacillus subtilis. J Bacteriol. 1992 Aug;174(16):5430–5435. doi: 10.1128/jb.174.16.5430-5435.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Buchanan C. E., Ling M. L. Isolation and sequence analysis of dacB, which encodes a sporulation-specific penicillin-binding protein in Bacillus subtilis. J Bacteriol. 1992 Mar;174(6):1717–1725. doi: 10.1128/jb.174.6.1717-1725.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Buchanan C. E., Neyman S. L. Correlation of penicillin-binding protein composition with different functions of two membranes in Bacillus subtilis forespores. J Bacteriol. 1986 Feb;165(2):498–503. doi: 10.1128/jb.165.2.498-503.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Bugaichuk U. D., Piggot P. J. Nucleotide sequence of the Bacillus subtilis developmental gene spoVE. J Gen Microbiol. 1986 Jul;132(7):1883–1890. doi: 10.1099/00221287-132-7-1883. [DOI] [PubMed] [Google Scholar]
  22. Burbulys D., Trach K. A., Hoch J. A. Initiation of sporulation in B. subtilis is controlled by a multicomponent phosphorelay. Cell. 1991 Feb 8;64(3):545–552. doi: 10.1016/0092-8674(91)90238-t. [DOI] [PubMed] [Google Scholar]
  23. Carlson H. C., Haldenwang W. G. The sigma E subunit of Bacillus subtilis RNA polymerase is present in both forespore and mother cell compartments. J Bacteriol. 1989 Apr;171(4):2216–2218. doi: 10.1128/jb.171.4.2216-2218.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Carter H. L., 3rd, Moran C. P., Jr New RNA polymerase sigma factor under spo0 control in Bacillus subtilis. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9438–9442. doi: 10.1073/pnas.83.24.9438. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Carter H. L., 3rd, Wang L. F., Doi R. H., Moran C. P., Jr rpoD operon promoter used by sigma H-RNA polymerase in Bacillus subtilis. J Bacteriol. 1988 Apr;170(4):1617–1621. doi: 10.1128/jb.170.4.1617-1621.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Chesnut R. S., Bookstein C., Hulett F. M. Separate promoters direct expression of phoAIII, a member of the Bacillus subtilis alkaline phosphatase multigene family, during phosphate starvation and sporulation. Mol Microbiol. 1991 Sep;5(9):2181–2190. doi: 10.1111/j.1365-2958.1991.tb02148.x. [DOI] [PubMed] [Google Scholar]
  27. Chibazakura T., Kawamura F., Takahashi H. Differential regulation of spo0A transcription in Bacillus subtilis: glucose represses promoter switching at the initiation of sporulation. J Bacteriol. 1991 Apr;173(8):2625–2632. doi: 10.1128/jb.173.8.2625-2632.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Clarke S., Lopez-Diaz I., Mandelstam J. Use of lacZ gene fusions to determine the dependence pattern of the sporulation gene spoIID in spo mutants of Bacillus subtilis. J Gen Microbiol. 1986 Nov;132(11):2987–2994. doi: 10.1099/00221287-132-11-2987. [DOI] [PubMed] [Google Scholar]
  29. Clarke S., Mandelstam J. Dissociation of an early event in sporulation from chromosome replication in Bacillus subtilis. J Gen Microbiol. 1980 Dec;121(2):487–490. doi: 10.1099/00221287-121-2-487. [DOI] [PubMed] [Google Scholar]
  30. Clarke S., Mandelstam J. Regulation of stage II of sporulation in Bacillus subtilis. J Gen Microbiol. 1987 Sep;133(9):2371–2380. doi: 10.1099/00221287-133-9-2371. [DOI] [PubMed] [Google Scholar]
  31. Connors M. J., Howard S., Hoch J., Setlow P. Determination of the chromosomal locations of four Bacillus subtilis genes which code for a family of small, acid-soluble spore proteins. J Bacteriol. 1986 May;166(2):412–416. doi: 10.1128/jb.166.2.412-416.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Coote J. G. Sporulation in Bacillus subtilis. Characterization of oligosporogenous mutants and comparison of their phenotypes with those of asporogenous mutants. J Gen Microbiol. 1972 Jun;71(1):1–15. doi: 10.1099/00221287-71-1-1. [DOI] [PubMed] [Google Scholar]
  33. Coppolecchia R., DeGrazia H., Moran C. P., Jr Deletion of spoIIAB blocks endospore formation in Bacillus subtilis at an early stage. J Bacteriol. 1991 Nov;173(21):6678–6685. doi: 10.1128/jb.173.21.6678-6685.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Cutting S., Driks A., Schmidt R., Kunkel B., Losick R. Forespore-specific transcription of a gene in the signal transduction pathway that governs Pro-sigma K processing in Bacillus subtilis. Genes Dev. 1991 Mar;5(3):456–466. doi: 10.1101/gad.5.3.456. [DOI] [PubMed] [Google Scholar]
  35. Cutting S., Mandelstam J. The nucleotide sequence and the transcription during sporulation of the gerE gene of Bacillus subtilis. J Gen Microbiol. 1986 Nov;132(11):3013–3024. doi: 10.1099/00221287-132-11-3013. [DOI] [PubMed] [Google Scholar]
  36. Cutting S., Oke V., Driks A., Losick R., Lu S., Kroos L. A forespore checkpoint for mother cell gene expression during development in B. subtilis. Cell. 1990 Jul 27;62(2):239–250. doi: 10.1016/0092-8674(90)90362-i. [DOI] [PubMed] [Google Scholar]
  37. Cutting S., Panzer S., Losick R. Regulatory studies on the promoter for a gene governing synthesis and assembly of the spore coat in Bacillus subtilis. J Mol Biol. 1989 May 20;207(2):393–404. doi: 10.1016/0022-2836(89)90262-3. [DOI] [PubMed] [Google Scholar]
  38. Cutting S., Roels S., Losick R. Sporulation operon spoIVF and the characterization of mutations that uncouple mother-cell from forespore gene expression in Bacillus subtilis. J Mol Biol. 1991 Oct 20;221(4):1237–1256. doi: 10.1016/0022-2836(91)90931-u. [DOI] [PubMed] [Google Scholar]
  39. Cutting S., Zheng L. B., Losick R. Gene encoding two alkali-soluble components of the spore coat from Bacillus subtilis. J Bacteriol. 1991 May;173(9):2915–2919. doi: 10.1128/jb.173.9.2915-2919.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Daniel R. A., Errington J. DNA sequence of the murE-murD region of Bacillus subtilis 168. J Gen Microbiol. 1993 Feb;139(2):361–370. doi: 10.1099/00221287-139-2-361. [DOI] [PubMed] [Google Scholar]
  41. Dawes I. W., Kay D., Mandelstam J. Determining effect of growth medium on the shape and position of daughter chromosomes and on sporulation in Bacillus subtilis. Nature. 1971 Apr 30;230(5296):567–569. doi: 10.1038/230567a0. [DOI] [PubMed] [Google Scholar]
  42. Dawes I. W., Mandelstam J. Sporulation of Bacillus subtilis in continuous culture. J Bacteriol. 1970 Sep;103(3):529–535. doi: 10.1128/jb.103.3.529-535.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Dion P., Mandelstam J. Germination properties as marker events characterizing later stages of Bacillus subtilis spore formation. J Bacteriol. 1980 Feb;141(2):786–792. doi: 10.1128/jb.141.2.786-792.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Donovan W., Zheng L. B., Sandman K., Losick R. Genes encoding spore coat polypeptides from Bacillus subtilis. J Mol Biol. 1987 Jul 5;196(1):1–10. doi: 10.1016/0022-2836(87)90506-7. [DOI] [PubMed] [Google Scholar]
  45. Driks A., Losick R. Compartmentalized expression of a gene under the control of sporulation transcription factor sigma E in Bacillus subtilis. Proc Natl Acad Sci U S A. 1991 Nov 15;88(22):9934–9938. doi: 10.1073/pnas.88.22.9934. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Dubnau D. Genetic competence in Bacillus subtilis. Microbiol Rev. 1991 Sep;55(3):395–424. doi: 10.1128/mr.55.3.395-424.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Dubnau E., Weir J., Nair G., Carter L., 3rd, Moran C., Jr, Smith I. Bacillus sporulation gene spo0H codes for sigma 30 (sigma H). J Bacteriol. 1988 Mar;170(3):1054–1062. doi: 10.1128/jb.170.3.1054-1062.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Dunn G., Torgersen D. M., Mandelstam J. Order of expression of genes affecting septum location during sporulation of Bacillus subtilis. J Bacteriol. 1976 Mar;125(3):776–779. doi: 10.1128/jb.125.3.776-779.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Errington J., Appleby L., Daniel R. A., Goodfellow H., Partridge S. R., Yudkin M. D. Structure and function of the spoIIIJ gene of Bacillus subtilis: a vegetatively expressed gene that is essential for sigma G activity at an intermediate stage of sporulation. J Gen Microbiol. 1992 Dec;138(12):2609–2618. doi: 10.1099/00221287-138-12-2609. [DOI] [PubMed] [Google Scholar]
  50. Errington J., Cutting S. M., Mandelstam J. Branched pattern of regulatory interactions between late sporulation genes in Bacillus subtilis. J Bacteriol. 1988 Feb;170(2):796–801. doi: 10.1128/jb.170.2.796-801.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  51. Errington J., Illing N. Establishment of cell-specific transcription during sporulation in Bacillus subtilis. Mol Microbiol. 1992 Mar;6(6):689–695. doi: 10.1111/j.1365-2958.1992.tb01517.x. [DOI] [PubMed] [Google Scholar]
  52. Errington J., Jones D. Cloning in Bacillus subtilis by transfection with bacteriophage vector phi 105J27: isolation and preliminary characterization of transducing phages for 23 sporulation loci. J Gen Microbiol. 1987 Mar;133(3):493–502. doi: 10.1099/00221287-133-3-493. [DOI] [PubMed] [Google Scholar]
  53. Errington J., Mandelstam J. Genetic and phenotypic characterization of a cluster of mutations in the spoVA locus of Bacillus subtilis. J Gen Microbiol. 1984 Aug;130(8):2115–2121. doi: 10.1099/00221287-130-8-2115. [DOI] [PubMed] [Google Scholar]
  54. Errington J., Mandelstam J. Use of a lacZ gene fusion to determine the dependence pattern and the spore compartment expression of sporulation operon spoVA in spo mutants of Bacillus subtilis. J Gen Microbiol. 1986 Nov;132(11):2977–2985. doi: 10.1099/00221287-132-11-2977. [DOI] [PubMed] [Google Scholar]
  55. Errington J., Mandelstam J. Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIA in spo mutants of Bacillus subtilis. J Gen Microbiol. 1986 Nov;132(11):2967–2976. doi: 10.1099/00221287-132-11-2967. [DOI] [PubMed] [Google Scholar]
  56. Errington J., Mandelstam J. Variety of sporulation phenotypes resulting from mutations in a single regulatory locus, spoIIA, in Bacillus subtilis. J Gen Microbiol. 1983 Jul;129(7):2091–2101. doi: 10.1099/00221287-129-7-2091. [DOI] [PubMed] [Google Scholar]
  57. Errington J., Wake R. G. Chromosome strand segregation during sporulation in Bacillus subtilis. Mol Microbiol. 1991 May;5(5):1145–1149. doi: 10.1111/j.1365-2958.1991.tb01887.x. [DOI] [PubMed] [Google Scholar]
  58. Errington J., Wootten L., Dunkerley J. C., Foulger D. Differential gene expression during sporulation in Bacillus subtilis: regulation of the spoVJ gene. Mol Microbiol. 1989 Aug;3(8):1053–1060. doi: 10.1111/j.1365-2958.1989.tb00255.x. [DOI] [PubMed] [Google Scholar]
  59. Fan N., Cutting S., Losick R. Characterization of the Bacillus subtilis sporulation gene spoVK. J Bacteriol. 1992 Feb;174(3):1053–1054. doi: 10.1128/jb.174.3.1053-1054.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  60. Feavers I. M., Foulkes J., Setlow B., Sun D., Nicholson W., Setlow P., Moir A. The regulation of transcription of the gerA spore germination operon of Bacillus subtilis. Mol Microbiol. 1990 Feb;4(2):275–282. doi: 10.1111/j.1365-2958.1990.tb00594.x. [DOI] [PubMed] [Google Scholar]
  61. Ferrari F. A., Trach K., Hoch J. A. Sequence analysis of the spo0B locus reveals a polycistronic transcription unit. J Bacteriol. 1985 Feb;161(2):556–562. doi: 10.1128/jb.161.2.556-562.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  62. Ferrari F. A., Trach K., LeCoq D., Spence J., Ferrari E., Hoch J. A. Characterization of the spo0A locus and its deduced product. Proc Natl Acad Sci U S A. 1985 May;82(9):2647–2651. doi: 10.1073/pnas.82.9.2647. [DOI] [PMC free article] [PubMed] [Google Scholar]
  63. Fort P., Errington J. Nucleotide sequence and complementation analysis of a polycistronic sporulation operon, spoVA, in Bacillus subtilis. J Gen Microbiol. 1985 May;131(5):1091–1105. doi: 10.1099/00221287-131-5-1091. [DOI] [PubMed] [Google Scholar]
  64. Fort P., Piggot P. J. Nucleotide sequence of sporulation locus spoIIA in Bacillus subtilis. J Gen Microbiol. 1984 Aug;130(8):2147–2153. doi: 10.1099/00221287-130-8-2147. [DOI] [PubMed] [Google Scholar]
  65. Foulger D., Errington J. Sequential activation of dual promoters by different sigma factors maintains spoVJ expression during successive developmental stages of Bacillus subtilis. Mol Microbiol. 1991 Jun;5(6):1363–1373. doi: 10.1111/j.1365-2958.1991.tb00783.x. [DOI] [PubMed] [Google Scholar]
  66. Foulger D., Errington J. The role of the sporulation gene spoIIIE in the regulation of prespore-specific gene expression in Bacillus subtilis. Mol Microbiol. 1989 Sep;3(9):1247–1255. doi: 10.1111/j.1365-2958.1989.tb00275.x. [DOI] [PubMed] [Google Scholar]
  67. Francesconi S. C., MacAlister T. J., Setlow B., Setlow P. Immunoelectron microscopic localization of small, acid-soluble spore proteins in sporulating cells of Bacillus subtilis. J Bacteriol. 1988 Dec;170(12):5963–5967. doi: 10.1128/jb.170.12.5963-5967.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  68. Fujita Y., Ramaley R., Freese E. Location and properties of glucose dehydrogenase in sporulating cells and spores of Bacillus subtilis. J Bacteriol. 1977 Oct;132(1):282–293. doi: 10.1128/jb.132.1.282-293.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  69. Fürbass R., Gocht M., Zuber P., Marahiel M. A. Interaction of AbrB, a transcriptional regulator from Bacillus subtilis with the promoters of the transition state-activated genes tycA and spoVG. Mol Gen Genet. 1991 Mar;225(3):347–354. doi: 10.1007/BF00261673. [DOI] [PubMed] [Google Scholar]
  70. Gardella T., Moyle H., Susskind M. M. A mutant Escherichia coli sigma 70 subunit of RNA polymerase with altered promoter specificity. J Mol Biol. 1989 Apr 20;206(4):579–590. doi: 10.1016/0022-2836(89)90567-6. [DOI] [PubMed] [Google Scholar]
  71. Gaur N. K., Cabane K., Smith I. Structure and expression of the Bacillus subtilis sin operon. J Bacteriol. 1988 Mar;170(3):1046–1053. doi: 10.1128/jb.170.3.1046-1053.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  72. Gaur N. K., Dubnau E., Smith I. Characterization of a cloned Bacillus subtilis gene that inhibits sporulation in multiple copies. J Bacteriol. 1986 Nov;168(2):860–869. doi: 10.1128/jb.168.2.860-869.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  73. Gaur N. K., Oppenheim J., Smith I. The Bacillus subtilis sin gene, a regulator of alternate developmental processes, codes for a DNA-binding protein. J Bacteriol. 1991 Jan;173(2):678–686. doi: 10.1128/jb.173.2.678-686.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  74. Gholamhoseinian A., Piggot P. J. Timing of spoII gene expression relative to septum formation during sporulation of Bacillus subtilis. J Bacteriol. 1989 Oct;171(10):5747–5749. doi: 10.1128/jb.171.10.5747-5749.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  75. Gholamhoseinian A., Shen Z., Wu J. J., Piggot P. Regulation of transcription of the cell division gene ftsA during sporulation of Bacillus subtilis. J Bacteriol. 1992 Jul;174(14):4647–4656. doi: 10.1128/jb.174.14.4647-4656.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  76. Gonzy-Tréboul G., Karmazyn-Campelli C., Stragier P. Developmental regulation of transcription of the Bacillus subtilis ftsAZ operon. J Mol Biol. 1992 Apr 20;224(4):967–979. doi: 10.1016/0022-2836(92)90463-t. [DOI] [PubMed] [Google Scholar]
  77. Grossman A. D. Integration of developmental signals and the initiation of sporulation in B. subtilis. Cell. 1991 Apr 5;65(1):5–8. doi: 10.1016/0092-8674(91)90353-z. [DOI] [PubMed] [Google Scholar]
  78. Grossman A. D., Losick R. Extracellular control of spore formation in Bacillus subtilis. Proc Natl Acad Sci U S A. 1988 Jun;85(12):4369–4373. doi: 10.1073/pnas.85.12.4369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  79. Guespin-Michel J. E. Phenotypic reversion in some early blocked sporulation mutants of Bacillus subtilis: isolation and phenotype identification of partial revertants. J Bacteriol. 1971 Oct;108(1):241–247. doi: 10.1128/jb.108.1.241-247.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  80. Guespin-Michel J. F. Phenotypic reversion in some early blocked sporulation mutants of Bacillus subtilis. Genetic study of polymyxin resistant partial revertants. Mol Gen Genet. 1971;112(3):243–254. [PubMed] [Google Scholar]
  81. Guzmán P., Westpheling J., Youngman P. Characterization of the promoter region of the Bacillus subtilis spoIIE operon. J Bacteriol. 1988 Apr;170(4):1598–1609. doi: 10.1128/jb.170.4.1598-1609.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  82. Hackett R. H., Setlow P. Enzymatic activity of precursors of Bacillus megaterium spore protease. J Bacteriol. 1983 Jan;153(1):375–378. doi: 10.1128/jb.153.1.375-378.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  83. Haldenwang W. G., Lang N., Losick R. A sporulation-induced sigma-like regulatory protein from B. subtilis. Cell. 1981 Feb;23(2):615–624. doi: 10.1016/0092-8674(81)90157-4. [DOI] [PubMed] [Google Scholar]
  84. Hanson R. S., Curry M. V., Garner J. V., Halvorson H. O. Mutants of Bacillus cereus strain T that produce thermoresistant spores lacking dipicolinate and have low levels of calcium. Can J Microbiol. 1972 Jul;18(7):1139–1143. doi: 10.1139/m72-175. [DOI] [PubMed] [Google Scholar]
  85. Harry E. J., Wake R. G. Cloning and expression of a Bacillus subtilis division initiation gene for which a homolog has not been identified in another organism. J Bacteriol. 1989 Dec;171(12):6835–6839. doi: 10.1128/jb.171.12.6835-6839.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  86. Hartwell L. H., Weinert T. A. Checkpoints: controls that ensure the order of cell cycle events. Science. 1989 Nov 3;246(4930):629–634. doi: 10.1126/science.2683079. [DOI] [PubMed] [Google Scholar]
  87. Healy J., Weir J., Smith I., Losick R. Post-transcriptional control of a sporulation regulatory gene encoding transcription factor sigma H in Bacillus subtilis. Mol Microbiol. 1991 Feb;5(2):477–487. doi: 10.1111/j.1365-2958.1991.tb02131.x. [DOI] [PubMed] [Google Scholar]
  88. Helmann J. D., Chamberlin M. J. Structure and function of bacterial sigma factors. Annu Rev Biochem. 1988;57:839–872. doi: 10.1146/annurev.bi.57.070188.004203. [DOI] [PubMed] [Google Scholar]
  89. Henriques A. O., de Lencastre H., Piggot P. J. A Bacillus subtilis morphogene cluster that includes spoVE is homologous to the mra region of Escherichia coli. Biochimie. 1992 Jul-Aug;74(7-8):735–748. doi: 10.1016/0300-9084(92)90146-6. [DOI] [PubMed] [Google Scholar]
  90. Hill S. H. SpoVH and spoVJ--new sporulation loci in Bacillus subtilis 168. J Gen Microbiol. 1983 Feb;129(2):293–302. doi: 10.1099/00221287-129-2-293. [DOI] [PubMed] [Google Scholar]
  91. Hitchins A. D., Slepecky R. A. Bacterial sporulation as a modified procaryotic cell division. Nature. 1969 Aug 23;223(5208):804–807. doi: 10.1038/223804a0. [DOI] [PubMed] [Google Scholar]
  92. Hoch J. A., Trach K., Kawamura F., Saito H. Identification of the transcriptional suppressor sof-1 as an alteration in the spo0A protein. J Bacteriol. 1985 Feb;161(2):552–555. doi: 10.1128/jb.161.2.552-555.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  93. Hogarth C., Ellar D. J. Energy-dependence of calcium accumulation during sporulation of Bacillus megaterium KM. Biochem J. 1979 Mar 15;178(3):627–632. doi: 10.1042/bj1780627. [DOI] [PMC free article] [PubMed] [Google Scholar]
  94. Holland S. K., Cutting S., Mandelstam J. The possible DNA-binding nature of the regulatory proteins, encoded by spoIID and gerE, involved in the sporulation of Bacillus subtilis. J Gen Microbiol. 1987 Sep;133(9):2381–2391. doi: 10.1099/00221287-133-9-2381. [DOI] [PubMed] [Google Scholar]
  95. Hranueli D., Piggot P. J., Mandelstam J. Statistical estimate of the total number of operons specific for Bacillus subtilis sporulation. J Bacteriol. 1974 Sep;119(3):684–690. doi: 10.1128/jb.119.3.684-690.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  96. Ikeda M., Sato T., Wachi M., Jung H. K., Ishino F., Kobayashi Y., Matsuhashi M. Structural similarity among Escherichia coli FtsW and RodA proteins and Bacillus subtilis SpoVE protein, which function in cell division, cell elongation, and spore formation, respectively. J Bacteriol. 1989 Nov;171(11):6375–6378. doi: 10.1128/jb.171.11.6375-6378.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  97. Ikeuchi T., Kudoh J., Tsunasawa S. Amino-terminal structure of spoOA protein and sequence homology with spoOF and spoOB proteins. Mol Gen Genet. 1986 Jun;203(3):371–376. doi: 10.1007/BF00422059. [DOI] [PubMed] [Google Scholar]
  98. Illing N., Errington J. Genetic regulation of morphogenesis in Bacillus subtilis: roles of sigma E and sigma F in prespore engulfment. J Bacteriol. 1991 May;173(10):3159–3169. doi: 10.1128/jb.173.10.3159-3169.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  99. Illing N., Errington J. The spoIIIA locus is not a major determinant of prespore-specific gene expression during sporulation in Bacillus subtilis. J Bacteriol. 1990 Dec;172(12):6930–6936. doi: 10.1128/jb.172.12.6930-6936.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  100. Illing N., Errington J. The spoIIIA operon of Bacillus subtilis defines a new temporal class of mother-cell-specific sporulation genes under the control of the sigma E form of RNA polymerase. Mol Microbiol. 1991 Aug;5(8):1927–1940. doi: 10.1111/j.1365-2958.1991.tb00816.x. [DOI] [PubMed] [Google Scholar]
  101. Illing N., Young M., Errington J. Use of integrational plasmid excision to identify cellular localization of gene expression during sporulation in Bacillus subtilis. J Bacteriol. 1990 Dec;172(12):6937–6941. doi: 10.1128/jb.172.12.6937-6941.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  102. Inoue T., Cech T. R. Secondary structure of the circular form of the Tetrahymena rRNA intervening sequence: a technique for RNA structure analysis using chemical probes and reverse transcriptase. Proc Natl Acad Sci U S A. 1985 Feb;82(3):648–652. doi: 10.1073/pnas.82.3.648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  103. Ireton K., Grossman A. D. Interactions among mutations that cause altered timing of gene expression during sporulation in Bacillus subtilis. J Bacteriol. 1992 May;174(10):3185–3195. doi: 10.1128/jb.174.10.3185-3195.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  104. Ishino F., Matsuhashi M. Peptidoglycan synthetic enzyme activities of highly purified penicillin-binding protein 3 in Escherichia coli: a septum-forming reaction sequence. Biochem Biophys Res Commun. 1981 Aug 14;101(3):905–911. doi: 10.1016/0006-291x(81)91835-0. [DOI] [PubMed] [Google Scholar]
  105. Ito J., Mildner G., Spizizen J. Early blocked asporogenous mutants of Bacillus subtilis 168. I. Isolation and characterization of mutants resistant to antibiotic(s) produced by sporulating Bacillus subtilis 168. Mol Gen Genet. 1971;112(2):104–109. doi: 10.1007/BF00267488. [DOI] [PubMed] [Google Scholar]
  106. Ito J. Pleiotropic nature of bacteriophage tolerant mutants obtained in early-blocked asporogenous mutants of Bacillus subtilis 168. Mol Gen Genet. 1973 Aug 10;124(2):97–106. doi: 10.1007/BF00265143. [DOI] [PubMed] [Google Scholar]
  107. Jaacks K. J., Healy J., Losick R., Grossman A. D. Identification and characterization of genes controlled by the sporulation-regulatory gene spo0H in Bacillus subtilis. J Bacteriol. 1989 Aug;171(8):4121–4129. doi: 10.1128/jb.171.8.4121-4129.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  108. James W., Mandelstam J. Protease production during sporulation of germination mutants of Bacillus subtilis and the cloning of a functional gerE gene. J Gen Microbiol. 1985 Sep;131(9):2421–2430. doi: 10.1099/00221287-131-9-2421. [DOI] [PubMed] [Google Scholar]
  109. Jenkinson H. F. Altered arrangement of proteins in the spore coat of a germination mutant of Bacillus subtilis. J Gen Microbiol. 1983 Jun;129(6):1945–1958. doi: 10.1099/00221287-129-6-1945. [DOI] [PubMed] [Google Scholar]
  110. Jenkinson H. F., Kay D., Mandelstam J. Temporal dissociation of late events in Bacillus subtilis sporulation from expression of genes that determine them. J Bacteriol. 1980 Feb;141(2):793–805. doi: 10.1128/jb.141.2.793-805.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  111. Jonas R. M., Haldenwang W. G. Influence of spo mutations on sigma E synthesis in Bacillus subtilis. J Bacteriol. 1989 Sep;171(9):5226–5228. doi: 10.1128/jb.171.9.5226-5228.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  112. Jonas R. M., Holt S. C., Haldenwang W. G. Effects of antibiotics on synthesis and persistence of sigma E in sporulating Bacillus subtilis. J Bacteriol. 1990 Aug;172(8):4616–4623. doi: 10.1128/jb.172.8.4616-4623.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  113. Jonas R. M., Peters H. K., 3rd, Haldenwang W. G. Phenotypes of Bacillus subtilis mutants altered in the precursor-specific region of sigma E. J Bacteriol. 1990 Aug;172(8):4178–4186. doi: 10.1128/jb.172.8.4178-4186.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  114. Jonas R. M., Weaver E. A., Kenney T. J., Moran C. P., Jr, Haldenwang W. G. The Bacillus subtilis spoIIG operon encodes both sigma E and a gene necessary for sigma E activation. J Bacteriol. 1988 Feb;170(2):507–511. doi: 10.1128/jb.170.2.507-511.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  115. Joris B., Dive G., Henriques A., Piggot P. J., Ghuysen J. M. The life-cycle proteins RodA of Escherichia coli and SpoVE of Bacillus subtilis have very similar primary structures. Mol Microbiol. 1990 Mar;4(3):513–517. doi: 10.1111/j.1365-2958.1990.tb00618.x. [DOI] [PubMed] [Google Scholar]
  116. Kapp N. V., Edwards C. W., Chesnut R. S., Hulett F. M. The Bacillus subtilis phoAIV gene: effects of in vitro inactivation on total alkaline phosphatase production. Gene. 1990 Nov 30;96(1):95–100. doi: 10.1016/0378-1119(90)90346-s. [DOI] [PubMed] [Google Scholar]
  117. Karmazyn-Campelli C., Bonamy C., Savelli B., Stragier P. Tandem genes encoding sigma-factors for consecutive steps of development in Bacillus subtilis. Genes Dev. 1989 Feb;3(2):150–157. doi: 10.1101/gad.3.2.150. [DOI] [PubMed] [Google Scholar]
  118. Kemp E. H., Sammons R. L., Moir A., Sun D., Setlow P. Analysis of transcriptional control of the gerD spore germination gene of Bacillus subtilis 168. J Bacteriol. 1991 Aug;173(15):4646–4652. doi: 10.1128/jb.173.15.4646-4652.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  119. Kenney T. J., Kirchman P. A., Moran C. P., Jr Gene encoding sigma E is transcribed from a sigma A-like promoter in Bacillus subtilis. J Bacteriol. 1988 Jul;170(7):3058–3064. doi: 10.1128/jb.170.7.3058-3064.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  120. Kenney T. J., Moran C. P., Jr Organization and regulation of an operon that encodes a sporulation-essential sigma factor in Bacillus subtilis. J Bacteriol. 1987 Jul;169(7):3329–3339. doi: 10.1128/jb.169.7.3329-3339.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  121. Kenney T. J., York K., Youngman P., Moran C. P., Jr Genetic evidence that RNA polymerase associated with sigma A factor uses a sporulation-specific promoter in Bacillus subtilis. Proc Natl Acad Sci U S A. 1989 Dec;86(23):9109–9113. doi: 10.1073/pnas.86.23.9109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  122. Keynan A., Berns A. A., Dunn G., Young M., Mandelstam J. Resporulation of outgrowing Bacillus subtilis spores. J Bacteriol. 1976 Oct;128(1):8–14. doi: 10.1128/jb.128.1.8-14.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  123. Kinney D. M., Bramucci M. G. Analysis of Bacillus subtilis sporulation with spore-converting bacteriophage PMB12. J Bacteriol. 1981 Mar;145(3):1281–1285. doi: 10.1128/jb.145.3.1281-1285.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  124. Klier A. F., Rapoport G. Genetics and regulation of carbohydrate catabolism in Bacillus. Annu Rev Microbiol. 1988;42:65–95. doi: 10.1146/annurev.mi.42.100188.000433. [DOI] [PubMed] [Google Scholar]
  125. Kroos L., Kunkel B., Losick R. Switch protein alters specificity of RNA polymerase containing a compartment-specific sigma factor. Science. 1989 Jan 27;243(4890):526–529. doi: 10.1126/science.2492118. [DOI] [PubMed] [Google Scholar]
  126. Kunkel B. Compartmentalized gene expression during sporulation in Bacillus subtilis. Trends Genet. 1991 May;7(5):167–172. doi: 10.1016/0168-9525(91)90381-y. [DOI] [PubMed] [Google Scholar]
  127. Kunkel B., Kroos L., Poth H., Youngman P., Losick R. Temporal and spatial control of the mother-cell regulatory gene spoIIID of Bacillus subtilis. Genes Dev. 1989 Nov;3(11):1735–1744. doi: 10.1101/gad.3.11.1735. [DOI] [PubMed] [Google Scholar]
  128. Kunkel B., Losick R., Stragier P. The Bacillus subtilis gene for the development transcription factor sigma K is generated by excision of a dispensable DNA element containing a sporulation recombinase gene. Genes Dev. 1990 Apr;4(4):525–535. doi: 10.1101/gad.4.4.525. [DOI] [PubMed] [Google Scholar]
  129. Kunkel B., Sandman K., Panzer S., Youngman P., Losick R. The promoter for a sporulation gene in the spoIVC locus of Bacillus subtilis and its use in studies of temporal and spatial control of gene expression. J Bacteriol. 1988 Aug;170(8):3513–3522. doi: 10.1128/jb.170.8.3513-3522.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  130. Kuroda A., Sekiguchi J. Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene. J Gen Microbiol. 1990 Nov;136(11):2209–2216. doi: 10.1099/00221287-136-11-2209. [DOI] [PubMed] [Google Scholar]
  131. LaBell T. L., Trempy J. E., Haldenwang W. G. Sporulation-specific sigma factor sigma 29 of Bacillus subtilis is synthesized from a precursor protein, P31. Proc Natl Acad Sci U S A. 1987 Apr;84(7):1784–1788. doi: 10.1073/pnas.84.7.1784. [DOI] [PMC free article] [PubMed] [Google Scholar]
  132. Lamont I. L., Mandelstam J. Identification of a new sporulation locus, spoIIIF, in Bacillus subtilis. J Gen Microbiol. 1984 May;130(5):1253–1261. doi: 10.1099/00221287-130-5-1253. [DOI] [PubMed] [Google Scholar]
  133. Lampel K. A., Uratani B., Chaudhry G. R., Ramaley R. F., Rudikoff S. Characterization of the developmentally regulated Bacillus subtilis glucose dehydrogenase gene. J Bacteriol. 1986 Apr;166(1):238–243. doi: 10.1128/jb.166.1.238-243.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  134. Lazarevic V., Margot P., Soldo B., Karamata D. Sequencing and analysis of the Bacillus subtilis lytRABC divergon: a regulatory unit encompassing the structural genes of the N-acetylmuramoyl-L-alanine amidase and its modifier. J Gen Microbiol. 1992 Sep;138(9):1949–1961. doi: 10.1099/00221287-138-9-1949. [DOI] [PubMed] [Google Scholar]
  135. Lewandoski M., Dubnau E., Smith I. Transcriptional regulation of the spo0F gene of Bacillus subtilis. J Bacteriol. 1986 Nov;168(2):870–877. doi: 10.1128/jb.168.2.870-877.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  136. Loshon C. A., Setlow P. Bacillus megaterium spore protease: purification, radioimmunoassay, and analysis of antigen level and localization during growth, sporulation, and spore germination. J Bacteriol. 1982 Apr;150(1):303–311. doi: 10.1128/jb.150.1.303-311.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  137. Loshon C. A., Swerdlow B. M., Setlow P. Bacillus megaterium spore protease. Synthesis and processing of precursor forms during sporulation and germination. J Biol Chem. 1982 Sep 25;257(18):10838–10845. [PubMed] [Google Scholar]
  138. Losick R., Pero J. Cascades of Sigma factors. Cell. 1981 Sep;25(3):582–584. doi: 10.1016/0092-8674(81)90164-1. [DOI] [PubMed] [Google Scholar]
  139. Losick R., Sonenshein A. L. Change in the template specificity of RNA polymerase during sporulation of Bacillus subtilis. Nature. 1969 Oct 4;224(5214):35–37. doi: 10.1038/224035a0. [DOI] [PubMed] [Google Scholar]
  140. Losick R., Stragier P. Crisscross regulation of cell-type-specific gene expression during development in B. subtilis. Nature. 1992 Feb 13;355(6361):601–604. doi: 10.1038/355601a0. [DOI] [PubMed] [Google Scholar]
  141. Losick R., Youngman P., Piggot P. J. Genetics of endospore formation in Bacillus subtilis. Annu Rev Genet. 1986;20:625–669. doi: 10.1146/annurev.ge.20.120186.003205. [DOI] [PubMed] [Google Scholar]
  142. Lu S., Halberg R., Kroos L. Processing of the mother-cell sigma factor, sigma K, may depend on events occurring in the forespore during Bacillus subtilis development. Proc Natl Acad Sci U S A. 1990 Dec;87(24):9722–9726. doi: 10.1073/pnas.87.24.9722. [DOI] [PMC free article] [PubMed] [Google Scholar]
  143. Mandelstam J., Higgs S. A. Induction of sporulation during synchronized chromosome replication in Bacillus subtilis. J Bacteriol. 1974 Oct;120(1):38–42. doi: 10.1128/jb.120.1.38-42.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  144. Mandic-Mulec I., Gaur N., Bai U., Smith I. Sin, a stage-specific repressor of cellular differentiation. J Bacteriol. 1992 Jun;174(11):3561–3569. doi: 10.1128/jb.174.11.3561-3569.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  145. Margolis P. S., Driks A., Losick R. Sporulation gene spoIIB from Bacillus subtilis. J Bacteriol. 1993 Jan;175(2):528–540. doi: 10.1128/jb.175.2.528-540.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  146. Margolis P., Driks A., Losick R. Establishment of cell type by compartmentalized activation of a transcription factor. Science. 1991 Oct 25;254(5031):562–565. doi: 10.1126/science.1948031. [DOI] [PubMed] [Google Scholar]
  147. Mason J. M., Fajardo-Cavazos P., Setlow P. Levels of mRNAs which code for small, acid-soluble spore proteins and their LacZ gene fusions in sporulating cells of Bacillus subtilis. Nucleic Acids Res. 1988 Jul 25;16(14A):6567–6583. doi: 10.1093/nar/16.14.6567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  148. Mason J. M., Hackett R. H., Setlow P. Regulation of expression of genes coding for small, acid-soluble proteins of Bacillus subtilis spores: studies using lacZ gene fusions. J Bacteriol. 1988 Jan;170(1):239–244. doi: 10.1128/jb.170.1.239-244.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  149. Mason J. M., Setlow P. Different small, acid-soluble proteins of the alpha/beta type have interchangeable roles in the heat and UV radiation resistance of Bacillus subtilis spores. J Bacteriol. 1987 Aug;169(8):3633–3637. doi: 10.1128/jb.169.8.3633-3637.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  150. Mason J. M., Setlow P. Essential role of small, acid-soluble spore proteins in resistance of Bacillus subtilis spores to UV light. J Bacteriol. 1986 Jul;167(1):174–178. doi: 10.1128/jb.167.1.174-178.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  151. Mason J. M., Setlow P. Expression of Bacillus megaterium and Bacillus subtilis small acid-soluble spore protein genes during stationary-phase growth of asporogenous B. subtilis mutants. J Bacteriol. 1984 Mar;157(3):931–933. doi: 10.1128/jb.157.3.931-933.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  152. Masuda E. S., Anaguchi H., Sato T., Takeuchi M., Kobayashi Y. Nucleotide sequence of the sporulation gene spoIIGA from Bacillus subtilis. Nucleic Acids Res. 1990 Feb 11;18(3):657–657. doi: 10.1093/nar/18.3.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  153. Masuda E. S., Anaguchi H., Yamada K., Kobayashi Y. Two developmental genes encoding sigma factor homologs are arranged in tandem in Bacillus subtilis. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7637–7641. doi: 10.1073/pnas.85.20.7637. [DOI] [PMC free article] [PubMed] [Google Scholar]
  154. Mathiopoulos C., Sonenshein A. L. Identification of Bacillus subtilis genes expressed early during sporulation. Mol Microbiol. 1989 Aug;3(8):1071–1081. doi: 10.1111/j.1365-2958.1989.tb00257.x. [DOI] [PubMed] [Google Scholar]
  155. Matsuhashi M., Wachi M., Ishino F. Machinery for cell growth and division: penicillin-binding proteins and other proteins. Res Microbiol. 1990 Jan;141(1):89–103. doi: 10.1016/0923-2508(90)90101-u. [DOI] [PubMed] [Google Scholar]
  156. Mitani T., Heinze J. E., Freese E. Induction of sporulation in Bacillus subtilis by decoyinine or hadacidin. Biochem Biophys Res Commun. 1977 Aug 8;77(3):1118–1125. doi: 10.1016/s0006-291x(77)80094-6. [DOI] [PubMed] [Google Scholar]
  157. Mohr S. C., Sokolov N. V., He C. M., Setlow P. Binding of small acid-soluble spore proteins from Bacillus subtilis changes the conformation of DNA from B to A. Proc Natl Acad Sci U S A. 1991 Jan 1;88(1):77–81. doi: 10.1073/pnas.88.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
  158. Moir A. Germination properties of a spore coat-defective mutant of Bacillus subtilis. J Bacteriol. 1981 Jun;146(3):1106–1116. doi: 10.1128/jb.146.3.1106-1116.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  159. Moir A., Smith D. A. The genetics of bacterial spore germination. Annu Rev Microbiol. 1990;44:531–553. doi: 10.1146/annurev.mi.44.100190.002531. [DOI] [PubMed] [Google Scholar]
  160. Moldover B., Piggot P. J., Yudkin M. D. Identification of the promoter and the transcriptional start site of the spoVA operon of Bacillus subtilis and Bacillus licheniformis. J Gen Microbiol. 1991 Mar;137(3):527–531. doi: 10.1099/00221287-137-3-527. [DOI] [PubMed] [Google Scholar]
  161. Moran C. P., Jr, Lang N., Banner C. D., Haldenwang W. G., Losick R. Promoter for a developmentally regulated gene in Bacillus subtilis. Cell. 1981 Sep;25(3):783–791. doi: 10.1016/0092-8674(81)90186-0. [DOI] [PubMed] [Google Scholar]
  162. Mysliwiec T. H., Errington J., Vaidya A. B., Bramucci M. G. The Bacillus subtilis spo0J gene: evidence for involvement in catabolite repression of sporulation. J Bacteriol. 1991 Mar;173(6):1911–1919. doi: 10.1128/jb.173.6.1911-1919.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  163. Nakatani Y., Nicholson W. L., Neitzke K. D., Setlow P., Freese E. Sigma-G RNA polymerase controls forespore-specific expression of the glucose dehydrogenase operon in Bacillus subtilis. Nucleic Acids Res. 1989 Feb 11;17(3):999–1017. doi: 10.1093/nar/17.3.999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  164. Nicholson W. L., Setlow B., Setlow P. Binding of DNA in vitro by a small, acid-soluble spore protein from Bacillus subtilis and the effect of this binding on DNA topology. J Bacteriol. 1990 Dec;172(12):6900–6906. doi: 10.1128/jb.172.12.6900-6906.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  165. Nicholson W. L., Setlow P. Dramatic increase in negative superhelicity of plasmid DNA in the forespore compartment of sporulating cells of Bacillus subtilis. J Bacteriol. 1990 Jan;172(1):7–14. doi: 10.1128/jb.172.1.7-14.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  166. Nicholson W. L., Sun D. X., Setlow B., Setlow P. Promoter specificity of sigma G-containing RNA polymerase from sporulating cells of Bacillus subtilis: identification of a group of forespore-specific promoters. J Bacteriol. 1989 May;171(5):2708–2718. doi: 10.1128/jb.171.5.2708-2718.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  167. Panzer S., Losick R., Sun D., Setlow P. Evidence for an additional temporal class of gene expression in the forespore compartment of sporulating Bacillus subtilis. J Bacteriol. 1989 Jan;171(1):561–564. doi: 10.1128/jb.171.1.561-564.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  168. Partridge S. R., Foulger D., Errington J. The role of sigma F in prespore-specific transcription in Bacillus subtilis. Mol Microbiol. 1991 Mar;5(3):757–767. doi: 10.1111/j.1365-2958.1991.tb00746.x. [DOI] [PubMed] [Google Scholar]
  169. Perego M., Cole S. P., Burbulys D., Trach K., Hoch J. A. Characterization of the gene for a protein kinase which phosphorylates the sporulation-regulatory proteins Spo0A and Spo0F of Bacillus subtilis. J Bacteriol. 1989 Nov;171(11):6187–6196. doi: 10.1128/jb.171.11.6187-6196.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  170. Perego M., Higgins C. F., Pearce S. R., Gallagher M. P., Hoch J. A. The oligopeptide transport system of Bacillus subtilis plays a role in the initiation of sporulation. Mol Microbiol. 1991 Jan;5(1):173–185. doi: 10.1111/j.1365-2958.1991.tb01838.x. [DOI] [PubMed] [Google Scholar]
  171. Perego M., Hoch J. A. Isolation and sequence of the spo0E gene: its role in initiation of sporulation in Bacillus subtilis. Mol Microbiol. 1987 Jul;1(1):125–132. doi: 10.1111/j.1365-2958.1987.tb00536.x. [DOI] [PubMed] [Google Scholar]
  172. Perego M., Hoch J. A. Negative regulation of Bacillus subtilis sporulation by the spo0E gene product. J Bacteriol. 1991 Apr;173(8):2514–2520. doi: 10.1128/jb.173.8.2514-2520.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  173. Perego M., Hoch J. A. Sequence analysis and regulation of the hpr locus, a regulatory gene for protease production and sporulation in Bacillus subtilis. J Bacteriol. 1988 Jun;170(6):2560–2567. doi: 10.1128/jb.170.6.2560-2567.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  174. Perego M., Spiegelman G. B., Hoch J. A. Structure of the gene for the transition state regulator, abrB: regulator synthesis is controlled by the spo0A sporulation gene in Bacillus subtilis. Mol Microbiol. 1988 Nov;2(6):689–699. doi: 10.1111/j.1365-2958.1988.tb00079.x. [DOI] [PubMed] [Google Scholar]
  175. Perego M., Wu J. J., Spiegelman G. B., Hoch J. A. Mutational dissociation of the positive and negative regulatory properties of the Spo0A sporulation transcription factor of Bacillus subtilis. Gene. 1991 Apr;100:207–212. doi: 10.1016/0378-1119(91)90368-l. [DOI] [PubMed] [Google Scholar]
  176. Piggot P. J., Coote J. G. Genetic aspects of bacterial endospore formation. Bacteriol Rev. 1976 Dec;40(4):908–962. doi: 10.1128/br.40.4.908-962.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  177. Piggot P. J., Curtis C. A., de Lencastre H. Use of integrational plasmid vectors to demonstrate the polycistronic nature of a transcriptional unit (spoIIA) required for sporulation of Bacillus subtilis. J Gen Microbiol. 1984 Aug;130(8):2123–2136. doi: 10.1099/00221287-130-8-2123. [DOI] [PubMed] [Google Scholar]
  178. Piggot P. J. Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporeulation operons. J Bacteriol. 1973 Jun;114(3):1241–1253. doi: 10.1128/jb.114.3.1241-1253.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  179. Popham D. L., Stragier P. Cloning, characterization, and expression of the spoVB gene of Bacillus subtilis. J Bacteriol. 1991 Dec;173(24):7942–7949. doi: 10.1128/jb.173.24.7942-7949.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  180. Predich M., Nair G., Smith I. Bacillus subtilis early sporulation genes kinA, spo0F, and spo0A are transcribed by the RNA polymerase containing sigma H. J Bacteriol. 1992 May;174(9):2771–2778. doi: 10.1128/jb.174.9.2771-2778.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  181. RYTER A. ETUDE MORPHOLOGIQUE DE LA SPORULATION DE BACILLUS SUBTILIS. Ann Inst Pasteur (Paris) 1965 Jan;108:40–60. [PubMed] [Google Scholar]
  182. Rather P. N., Coppolecchia R., DeGrazia H., Moran C. P., Jr Negative regulator of sigma G-controlled gene expression in stationary-phase Bacillus subtilis. J Bacteriol. 1990 Feb;172(2):709–715. doi: 10.1128/jb.172.2.709-715.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  183. Rather P. N., Moran C. P., Jr Compartment-specific transcription in Bacillus subtilis: identification of the promoter for gdh. J Bacteriol. 1988 Nov;170(11):5086–5092. doi: 10.1128/jb.170.11.5086-5092.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  184. Reeve J. N., Mendelson N. H., Coyne S. I., Hallock L. L., Cole R. M. Minicells of Bacillus subtilis. J Bacteriol. 1973 May;114(2):860–873. doi: 10.1128/jb.114.2.860-873.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  185. Ricca E., Cutting S., Losick R. Characterization of bofA, a gene involved in intercompartmental regulation of pro-sigma K processing during sporulation in Bacillus subtilis. J Bacteriol. 1992 May;174(10):3177–3184. doi: 10.1128/jb.174.10.3177-3184.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  186. Robertson J. B., Gocht M., Marahiel M. A., Zuber P. AbrB, a regulator of gene expression in Bacillus, interacts with the transcription initiation regions of a sporulation gene and an antibiotic biosynthesis gene. Proc Natl Acad Sci U S A. 1989 Nov;86(21):8457–8461. doi: 10.1073/pnas.86.21.8457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  187. Roels S., Driks A., Losick R. Characterization of spoIVA, a sporulation gene involved in coat morphogenesis in Bacillus subtilis. J Bacteriol. 1992 Jan;174(2):575–585. doi: 10.1128/jb.174.2.575-585.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  188. Rong S., Rosenkrantz M. S., Sonenshein A. L. Transcriptional control of the Bacillus subtilis spoIID gene. J Bacteriol. 1986 Mar;165(3):771–779. doi: 10.1128/jb.165.3.771-779.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  189. Rosenbluh A., Banner C. D., Losick R., Fitz-James P. C. Identification of a new developmental locus in Bacillus subtilis by construction of a deletion mutation in a cloned gene under sporulation control. J Bacteriol. 1981 Oct;148(1):341–351. doi: 10.1128/jb.148.1.341-351.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  190. Rudner D. Z., LeDeaux J. R., Ireton K., Grossman A. D. The spo0K locus of Bacillus subtilis is homologous to the oligopeptide permease locus and is required for sporulation and competence. J Bacteriol. 1991 Feb;173(4):1388–1398. doi: 10.1128/jb.173.4.1388-1398.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  191. Sammons R. L., Slynn G. M., Smith D. A. Genetical and molecular studies on gerM, a new developmental locus of Bacillus subtilis. J Gen Microbiol. 1987 Dec;133(12):3299–3312. doi: 10.1099/00221287-133-12-3299. [DOI] [PubMed] [Google Scholar]
  192. Sandman K., Kroos L., Cutting S., Youngman P., Losick R. Identification of the promoter for a spore coat protein gene in Bacillus subtilis and studies on the regulation of its induction at a late stage of sporulation. J Mol Biol. 1988 Apr 5;200(3):461–473. doi: 10.1016/0022-2836(88)90536-0. [DOI] [PubMed] [Google Scholar]
  193. Sandman K., Losick R., Youngman P. Genetic analysis of Bacillus subtilis spo mutations generated by Tn917-mediated insertional mutagenesis. Genetics. 1987 Dec;117(4):603–617. doi: 10.1093/genetics/117.4.603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  194. Sastry K. J., Srivastava O. P., Millet J., FitzJames P. C., Aronson A. I. Characterization of Bacillus subtilis mutants with a temperature-sensitive intracellular protease. J Bacteriol. 1983 Jan;153(1):511–519. doi: 10.1128/jb.153.1.511-519.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  195. Sato T., Samori Y., Kobayashi Y. The cisA cistron of Bacillus subtilis sporulation gene spoIVC encodes a protein homologous to a site-specific recombinase. J Bacteriol. 1990 Feb;172(2):1092–1098. doi: 10.1128/jb.172.2.1092-1098.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  196. Sato T., Theeragool G., Yamamoto T., Okamoto M., Kobayashi Y. Revised nucleotide sequence of the sporulation gene spoVE from Bacillus subtilis. Nucleic Acids Res. 1990 Jul 11;18(13):4021–4021. doi: 10.1093/nar/18.13.4021. [DOI] [PMC free article] [PubMed] [Google Scholar]
  197. Satola S. W., Baldus J. M., Moran C. P., Jr Binding of Spo0A stimulates spoIIG promoter activity in Bacillus subtilis. J Bacteriol. 1992 Mar;174(5):1448–1453. doi: 10.1128/jb.174.5.1448-1453.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  198. Satola S., Kirchman P. A., Moran C. P., Jr Spo0A binds to a promoter used by sigma A RNA polymerase during sporulation in Bacillus subtilis. Proc Natl Acad Sci U S A. 1991 May 15;88(10):4533–4537. doi: 10.1073/pnas.88.10.4533. [DOI] [PMC free article] [PubMed] [Google Scholar]
  199. Savva D., Mandelstam J. Synthesis of spoIIA and spoVA mRNA in Bacillus subtilis. J Gen Microbiol. 1986 Nov;132(11):3005–3011. doi: 10.1099/00221287-132-11-3005. [DOI] [PubMed] [Google Scholar]
  200. Schmidt R., Margolis P., Duncan L., Coppolecchia R., Moran C. P., Jr, Losick R. Control of developmental transcription factor sigma F by sporulation regulatory proteins SpoIIAA and SpoIIAB in Bacillus subtilis. Proc Natl Acad Sci U S A. 1990 Dec;87(23):9221–9225. doi: 10.1073/pnas.87.23.9221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  201. Sekiguchi J., Ezaki B., Kodama K., Akamatsu T. Molecular cloning of a gene affecting the autolysin level and flagellation in Bacillus subtilis. J Gen Microbiol. 1988 Jun;134(6):1611–1621. doi: 10.1099/00221287-134-6-1611. [DOI] [PubMed] [Google Scholar]
  202. Setlow B., Hand A. R., Setlow P. Synthesis of a Bacillus subtilis small, acid-soluble spore protein in Escherichia coli causes cell DNA to assume some characteristics of spore DNA. J Bacteriol. 1991 Mar;173(5):1642–1653. doi: 10.1128/jb.173.5.1642-1653.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  203. Setlow B., Magill N., Febbroriello P., Nakhimovsky L., Koppel D. E., Setlow P. Condensation of the forespore nucleoid early in sporulation of Bacillus species. J Bacteriol. 1991 Oct;173(19):6270–6278. doi: 10.1128/jb.173.19.6270-6278.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  204. Setlow B., Setlow P. Thymine-containing dimers as well as spore photoproducts are found in ultraviolet-irradiated Bacillus subtilis spores that lack small acid-soluble proteins. Proc Natl Acad Sci U S A. 1987 Jan;84(2):421–423. doi: 10.1073/pnas.84.2.421. [DOI] [PMC free article] [PubMed] [Google Scholar]
  205. Setlow P. I will survive: protecting and repairing spore DNA. J Bacteriol. 1992 May;174(9):2737–2741. doi: 10.1128/jb.174.9.2737-2741.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  206. Setlow P. Small, acid-soluble spore proteins of Bacillus species: structure, synthesis, genetics, function, and degradation. Annu Rev Microbiol. 1988;42:319–338. doi: 10.1146/annurev.mi.42.100188.001535. [DOI] [PubMed] [Google Scholar]
  207. Siegele D. A., Hu J. C., Walter W. A., Gross C. A. Altered promoter recognition by mutant forms of the sigma 70 subunit of Escherichia coli RNA polymerase. J Mol Biol. 1989 Apr 20;206(4):591–603. doi: 10.1016/0022-2836(89)90568-8. [DOI] [PubMed] [Google Scholar]
  208. Silver-Mysliwiec T. H., Bramucci M. G. Bacteriophage-enhanced sporulation: comparison of spore-converting bacteriophages PMB12 and SP10. J Bacteriol. 1990 Apr;172(4):1948–1953. doi: 10.1128/jb.172.4.1948-1953.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  209. Singh R. P., Setlow B., Setlow P. Levels of small molecules and enzymes in the mother cell compartment and the forespore of sporulating Bacillus megaterium. J Bacteriol. 1977 Jun;130(3):1130–1138. doi: 10.1128/jb.130.3.1130-1138.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  210. Slack F. J., Mueller J. P., Strauch M. A., Mathiopoulos C., Sonenshein A. L. Transcriptional regulation of a Bacillus subtilis dipeptide transport operon. Mol Microbiol. 1991 Aug;5(8):1915–1925. doi: 10.1111/j.1365-2958.1991.tb00815.x. [DOI] [PubMed] [Google Scholar]
  211. Sloma A., Rufo G. A., Jr, Rudolph C. F., Sullivan B. J., Theriault K. A., Pero J. Bacillopeptidase F of Bacillus subtilis: purification of the protein and cloning of the gene. J Bacteriol. 1990 Sep;172(9):5520–5521. doi: 10.1128/jb.172.9.5520-5521.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  212. Sowell M. O., Buchanan C. E. Changes in penicillin-binding proteins during sporulation of Bacillus subtilis. J Bacteriol. 1983 Mar;153(3):1331–1337. doi: 10.1128/jb.153.3.1331-1337.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  213. Spiegelman G., Van Hoy B., Perego M., Day J., Trach K., Hoch J. A. Structural alterations in the Bacillus subtilis Spo0A regulatory protein which suppress mutations at several spo0 loci. J Bacteriol. 1990 Sep;172(9):5011–5019. doi: 10.1128/jb.172.9.5011-5019.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  214. Stephens M. A., Lang N., Sandman K., Losick R. A promoter whose utilization is temporally regulated during sporulation in Bacillus subtilis. J Mol Biol. 1984 Jul 5;176(3):333–348. doi: 10.1016/0022-2836(84)90493-5. [DOI] [PubMed] [Google Scholar]
  215. Sterlini J. M., Mandelstam J. Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochem J. 1969 Jun;113(1):29–37. doi: 10.1042/bj1130029. [DOI] [PMC free article] [PubMed] [Google Scholar]
  216. Stevens C. M., Daniel R., Illing N., Errington J. Characterization of a sporulation gene, spoIVA, involved in spore coat morphogenesis in Bacillus subtilis. J Bacteriol. 1992 Jan;174(2):586–594. doi: 10.1128/jb.174.2.586-594.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  217. Stevens C. M., Errington J. Differential gene expression during sporulation in Bacillus subtilis: structure and regulation of the spoIIID gene. Mol Microbiol. 1990 Apr;4(4):543–551. doi: 10.1111/j.1365-2958.1990.tb00622.x. [DOI] [PubMed] [Google Scholar]
  218. Stock J. B., Ninfa A. J., Stock A. M. Protein phosphorylation and regulation of adaptive responses in bacteria. Microbiol Rev. 1989 Dec;53(4):450–490. doi: 10.1128/mr.53.4.450-490.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  219. Stragier P., Bonamy C., Karmazyn-Campelli C. Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression. Cell. 1988 Mar 11;52(5):697–704. doi: 10.1016/0092-8674(88)90407-2. [DOI] [PubMed] [Google Scholar]
  220. Stragier P., Bouvier J., Bonamy C., Szulmajster J. A developmental gene product of Bacillus subtilis homologous to the sigma factor of Escherichia coli. Nature. 1984 Nov 22;312(5992):376–378. doi: 10.1038/312376a0. [DOI] [PubMed] [Google Scholar]
  221. Stragier P. Comment on 'Duplicated sporulation genes in bacteria' by J. Errington, P. Fort and J. Mandelstam. FEBS Lett. 1986 Jan 20;195(1-2):9–11. doi: 10.1016/0014-5793(86)80119-3. [DOI] [PubMed] [Google Scholar]
  222. Stragier P., Kunkel B., Kroos L., Losick R. Chromosomal rearrangement generating a composite gene for a developmental transcription factor. Science. 1989 Jan 27;243(4890):507–512. doi: 10.1126/science.2536191. [DOI] [PubMed] [Google Scholar]
  223. Stragier P., Losick R. Cascades of sigma factors revisited. Mol Microbiol. 1990 Nov;4(11):1801–1806. doi: 10.1111/j.1365-2958.1990.tb02028.x. [DOI] [PubMed] [Google Scholar]
  224. Strauch M. A., Perego M., Burbulys D., Hoch J. A. The transition state transcription regulator AbrB of Bacillus subtilis is autoregulated during vegetative growth. Mol Microbiol. 1989 Sep;3(9):1203–1209. doi: 10.1111/j.1365-2958.1989.tb00270.x. [DOI] [PubMed] [Google Scholar]
  225. Strauch M. A., Spiegelman G. B., Perego M., Johnson W. C., Burbulys D., Hoch J. A. The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein. EMBO J. 1989 May;8(5):1615–1621. doi: 10.1002/j.1460-2075.1989.tb03546.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  226. Strauch M., Webb V., Spiegelman G., Hoch J. A. The SpoOA protein of Bacillus subtilis is a repressor of the abrB gene. Proc Natl Acad Sci U S A. 1990 Mar;87(5):1801–1805. doi: 10.1073/pnas.87.5.1801. [DOI] [PMC free article] [PubMed] [Google Scholar]
  227. Sun D. X., Cabrera-Martinez R. M., Setlow P. Control of transcription of the Bacillus subtilis spoIIIG gene, which codes for the forespore-specific transcription factor sigma G. J Bacteriol. 1991 May;173(9):2977–2984. doi: 10.1128/jb.173.9.2977-2984.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  228. Sun D. X., Setlow P. Cloning, nucleotide sequence, and expression of the Bacillus subtilis ans operon, which codes for L-asparaginase and L-aspartase. J Bacteriol. 1991 Jun;173(12):3831–3845. doi: 10.1128/jb.173.12.3831-3845.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  229. Sun D. X., Stragier P., Setlow P. Identification of a new sigma-factor involved in compartmentalized gene expression during sporulation of Bacillus subtilis. Genes Dev. 1989 Feb;3(2):141–149. doi: 10.1101/gad.3.2.141. [DOI] [PubMed] [Google Scholar]
  230. Sun D., Fajardo-Cavazos P., Sussman M. D., Tovar-Rojo F., Cabrera-Martinez R. M., Setlow P. Effect of chromosome location of Bacillus subtilis forespore genes on their spo gene dependence and transcription by E sigma F: identification of features of good E sigma F-dependent promoters. J Bacteriol. 1991 Dec;173(24):7867–7874. doi: 10.1128/jb.173.24.7867-7874.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  231. Sussman M. D., Setlow P. Cloning, nucleotide sequence, and regulation of the Bacillus subtilis gpr gene, which codes for the protease that initiates degradation of small, acid-soluble proteins during spore germination. J Bacteriol. 1991 Jan;173(1):291–300. doi: 10.1128/jb.173.1.291-300.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  232. Tapscott S. J., Davis R. L., Thayer M. J., Cheng P. F., Weintraub H., Lassar A. B. MyoD1: a nuclear phosphoprotein requiring a Myc homology region to convert fibroblasts to myoblasts. Science. 1988 Oct 21;242(4877):405–411. doi: 10.1126/science.3175662. [DOI] [PubMed] [Google Scholar]
  233. Tatti K. M., Jones C. H., Moran C. P., Jr Genetic evidence for interaction of sigma E with the spoIIID promoter in Bacillus subtilis. J Bacteriol. 1991 Dec;173(24):7828–7833. doi: 10.1128/jb.173.24.7828-7833.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  234. Tipper D. J., Linnett P. E. Distribution of peptidoglycan synthetase activities between sporangia and forespores in sporulating cells of Bacillus sphaericus. J Bacteriol. 1976 Apr;126(1):213–221. doi: 10.1128/jb.126.1.213-221.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  235. Todd J. A., Bone E. J., Ellar D. J. The sporulation-specific penicillin-binding protein 5a from Bacillus subtilis is a DD-carboxypeptidase in vitro. Biochem J. 1985 Sep 15;230(3):825–828. doi: 10.1042/bj2300825. [DOI] [PMC free article] [PubMed] [Google Scholar]
  236. Todd J. A., Roberts A. N., Johnstone K., Piggot P. J., Winter G., Ellar D. J. Reduced heat resistance of mutant spores after cloning and mutagenesis of the Bacillus subtilis gene encoding penicillin-binding protein 5. J Bacteriol. 1986 Jul;167(1):257–264. doi: 10.1128/jb.167.1.257-264.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  237. Trach K., Chapman J. W., Piggot P., LeCoq D., Hoch J. A. Complete sequence and transcriptional analysis of the spo0F region of the Bacillus subtilis chromosome. J Bacteriol. 1988 Sep;170(9):4194–4208. doi: 10.1128/jb.170.9.4194-4208.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  238. Trach K., Hoch J. A. The Bacillus subtilis spo0B stage 0 sporulation operon encodes an essential GTP-binding protein. J Bacteriol. 1989 Mar;171(3):1362–1371. doi: 10.1128/jb.171.3.1362-1371.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  239. Trempy J. E., Bonamy C., Szulmajster J., Haldenwang W. G. Bacillus subtilis sigma factor sigma 29 is the product of the sporulation-essential gene spoIIG. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4189–4192. doi: 10.1073/pnas.82.12.4189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  240. Trempy J. E., Morrison-Plummer J., Haldenwang W. G. Synthesis of sigma 29, an RNA polymerase specificity determinant, is a developmentally regulated event in Bacillus subtilis. J Bacteriol. 1985 Jan;161(1):340–346. doi: 10.1128/jb.161.1.340-346.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  241. Trowsdale J., Chen S. M., Hoch J. A. Genetic analysis of a class of polymyxin resistant partial revertants of stage O sporulation mutants of Bacillus subtilis: map of the chromosome region near the origin of replication. Mol Gen Genet. 1979 May 23;173(1):61–70. doi: 10.1007/BF00267691. [DOI] [PubMed] [Google Scholar]
  242. Turner S. M., Errington J., Mandelstam J. Use of a lacZ gene fusion to determine the dependence pattern of sporulation operon spoIIIC in spo mutants of Bacillus subtilis: a branched pathway of expression of sporulation operons. J Gen Microbiol. 1986 Nov;132(11):2995–3003. doi: 10.1099/00221287-132-11-2995. [DOI] [PubMed] [Google Scholar]
  243. Van Hoy B. E., Hoch J. A. Characterization of the spoIVB and recN loci of Bacillus subtilis. J Bacteriol. 1990 Mar;172(3):1306–1311. doi: 10.1128/jb.172.3.1306-1311.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  244. Waites W. M., Kay D., Dawes I. W., Wood D. A., Warren S. C., Mandelstam J. Sporulation in Bacillus subtilis. Correlation of biochemical events with morphological changes in asporogenous mutants. Biochem J. 1970 Jul;118(4):667–676. doi: 10.1042/bj1180667. [DOI] [PMC free article] [PubMed] [Google Scholar]
  245. Warburg R. J., Buchanan C. E., Parent K., Halvorson H. O. A detailed study of gerJ mutants of Bacillus subtilis. J Gen Microbiol. 1986 Aug;132(8):2309–2319. doi: 10.1099/00221287-132-8-2309. [DOI] [PubMed] [Google Scholar]
  246. Ward J. E., Jr, Lutkenhaus J. Overproduction of FtsZ induces minicell formation in E. coli. Cell. 1985 Oct;42(3):941–949. doi: 10.1016/0092-8674(85)90290-9. [DOI] [PubMed] [Google Scholar]
  247. Warren S. C. Sporulation in Bacillus subtilis. Biochemical changes. Biochem J. 1968 Oct;109(5):811–818. doi: 10.1042/bj1090811. [DOI] [PMC free article] [PubMed] [Google Scholar]
  248. Warth A. D., Strominger J. L. Structure of the peptidoglycan from spores of Bacillus subtilis. Biochemistry. 1972 Apr 11;11(8):1389–1396. doi: 10.1021/bi00758a010. [DOI] [PubMed] [Google Scholar]
  249. Waxman D. J., Strominger J. L. Penicillin-binding proteins and the mechanism of action of beta-lactam antibiotics. Annu Rev Biochem. 1983;52:825–869. doi: 10.1146/annurev.bi.52.070183.004141. [DOI] [PubMed] [Google Scholar]
  250. Weir J., Predich M., Dubnau E., Nair G., Smith I. Regulation of spo0H, a gene coding for the Bacillus subtilis sigma H factor. J Bacteriol. 1991 Jan;173(2):521–529. doi: 10.1128/jb.173.2.521-529.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  251. Wu J. J., Howard M. G., Piggot P. J. Regulation of transcription of the Bacillus subtilis spoIIA locus. J Bacteriol. 1989 Feb;171(2):692–698. doi: 10.1128/jb.171.2.692-698.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  252. Wu J. J., Piggot P. J., Tatti K. M., Moran C. P., Jr Transcription of the Bacillus subtilis spoIIA locus. Gene. 1991 May 15;101(1):113–116. doi: 10.1016/0378-1119(91)90231-y. [DOI] [PubMed] [Google Scholar]
  253. Wu J. J., Schuch R., Piggot P. J. Characterization of a Bacillus subtilis sporulation operon that includes genes for an RNA polymerase sigma factor and for a putative DD-carboxypeptidase. J Bacteriol. 1992 Aug;174(15):4885–4892. doi: 10.1128/jb.174.15.4885-4892.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  254. Yamashita S., Kawamura F., Yoshikawa H., Takahashi H., Kobayashi Y., Saito H. Dissection of the expression signals of the spoA gene of Bacillus subtilis: glucose represses sporulation-specific expression. J Gen Microbiol. 1989 May;135(5):1335–1345. doi: 10.1099/00221287-135-5-1335. [DOI] [PubMed] [Google Scholar]
  255. Yamashita S., Yoshikawa H., Kawamura F., Takahashi H., Yamamoto T., Kobayashi Y., Saito H. The effect of spo0 mutations on the expression of spo0A- and spo0F-lacZ fusions. Mol Gen Genet. 1986 Oct;205(1):28–33. doi: 10.1007/BF02428029. [DOI] [PubMed] [Google Scholar]
  256. York K., Kenney T. J., Satola S., Moran C. P., Jr, Poth H., Youngman P. Spo0A controls the sigma A-dependent activation of Bacillus subtilis sporulation-specific transcription unit spoIIE. J Bacteriol. 1992 Apr;174(8):2648–2658. doi: 10.1128/jb.174.8.2648-2658.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  257. Young M., Mandelstam J. Early events during bacterial endospore formation. Adv Microb Physiol. 1979;20:103-62, 321-3. doi: 10.1016/s0065-2911(08)60207-6. [DOI] [PubMed] [Google Scholar]
  258. Young M. Use of temperature-sensitive mutants to study gene expression during sporulation in Bacillus subtilis. J Bacteriol. 1976 May;126(2):928–936. doi: 10.1128/jb.126.2.928-936.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  259. Yudkin M. D., Harrison D. Effect of precisely identified mutations in the spoIIAC gene of Bacillus subtilis on the toxicity of the sigma-like gene product to Escherichia coli. Mol Gen Genet. 1987 Sep;209(2):333–334. doi: 10.1007/BF00329662. [DOI] [PubMed] [Google Scholar]
  260. Yudkin M. D. The sigma-like product of sporulation gene spoIIAC of Bacillus subtilis is toxic to Escherichia coli. Mol Gen Genet. 1986 Jan;202(1):55–57. doi: 10.1007/BF00330516. [DOI] [PubMed] [Google Scholar]
  261. Yura T., Mori H., Nagai H., Nagata T., Ishihama A., Fujita N., Isono K., Mizobuchi K., Nakata A. Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region. Nucleic Acids Res. 1992 Jul 11;20(13):3305–3308. doi: 10.1093/nar/20.13.3305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  262. Zheng L. B., Donovan W. P., Fitz-James P. C., Losick R. Gene encoding a morphogenic protein required in the assembly of the outer coat of the Bacillus subtilis endospore. Genes Dev. 1988 Aug;2(8):1047–1054. doi: 10.1101/gad.2.8.1047. [DOI] [PubMed] [Google Scholar]
  263. Zheng L. B., Losick R. Cascade regulation of spore coat gene expression in Bacillus subtilis. J Mol Biol. 1990 Apr 20;212(4):645–660. doi: 10.1016/0022-2836(90)90227-d. [DOI] [PubMed] [Google Scholar]
  264. Zheng L., Halberg R., Roels S., Ichikawa H., Kroos L., Losick R. Sporulation regulatory protein GerE from Bacillus subtilis binds to and can activate or repress transcription from promoters for mother-cell-specific genes. J Mol Biol. 1992 Aug 20;226(4):1037–1050. doi: 10.1016/0022-2836(92)91051-p. [DOI] [PubMed] [Google Scholar]
  265. Zuber P., Healy J., Carter H. L., 3rd, Cutting S., Moran C. P., Jr, Losick R. Mutation changing the specificity of an RNA polymerase sigma factor. J Mol Biol. 1989 Apr 20;206(4):605–614. doi: 10.1016/0022-2836(89)90569-x. [DOI] [PubMed] [Google Scholar]
  266. Zuber P., Losick R. Role of AbrB in Spo0A- and Spo0B-dependent utilization of a sporulation promoter in Bacillus subtilis. J Bacteriol. 1987 May;169(5):2223–2230. doi: 10.1128/jb.169.5.2223-2230.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  267. Zuber P., Losick R. Use of a lacZ fusion to study the role of the spoO genes of Bacillus subtilis in developmental regulation. Cell. 1983 Nov;35(1):275–283. doi: 10.1016/0092-8674(83)90230-1. [DOI] [PubMed] [Google Scholar]
  268. Zuberi A. R., Moir A., Feavers I. M. The nucleotide sequence and gene organization of the gerA spore germination operon of Bacillus subtilis 168. Gene. 1987;51(1):1–11. doi: 10.1016/0378-1119(87)90468-9. [DOI] [PubMed] [Google Scholar]
  269. de Lencastre H., Piggot P. J. Identification of different sites of expression for spo loci by transformation of Bacillus subtilis. J Gen Microbiol. 1979 Oct;114(2):377–389. doi: 10.1099/00221287-114-2-377. [DOI] [PubMed] [Google Scholar]

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