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. 1967 Apr;1(2):302–307. doi: 10.1128/jvi.1.2.302-307.1967

Purification and Properties of Reovirus Ribonucleic Acid

W J Iglewski 1, R M Franklin 1
PMCID: PMC375228  PMID: 4318949

Abstract

NaClO4 was employed in a technique for the rapid extraction of reovirus ribonucleic acid (RNA). The extracted RNA, which was purified in a Cs2SO4 equilibrium density gradient, had a buoyant density of 1.61 g/cm3 and a sedimentation coefficient of 15S in a 7 to 20% sucrose gradient. It was 90% resistant to ribonuclease in a solution of high ionic strength (0.1 m NaCl). The sensitivity of reovirus RNA to ribonuclease increased with decreasing ionic strength. The thermal denaturation transition of the RNA began at 78 C and was complete at 85 C. The Tm of the transition was 81 C in 0.01 m tris(hydroxymethyl)aminomethane buffer (pH 7.2) containing 0.001 m ethylenediaminetetraacetate. Thermal denaturation of reovirus RNA resulted in the formation of three ribonuclease-sensitive fractions. Denaturation at 25 C in the presence of dimethyl sulfoxide resulted in the formation of two ribonuclease-sensitive fractions.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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