Abstract
A new zonal centrifuge rotor (B-IX) which combines continuous sample flow centrifugation with isopycnic banding has been used to isolate and concentrate respiratory syncytial virus from liter volumes of culture fluid. This isolation technique utilizes a sucrose density gradient to trap and isopycnically band the virus particles, and permits recovery of the particles from the rotor in an unaggregated condition.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson N. G., Barringer H. P., Amburgey J. W., Jr, Cline G. B., Nunley C. E., Berman A. S. Continuous-flow centrifugation combined with isopycnic banding: rotors B-8 and B-IX. Natl Cancer Inst Monogr. 1966 Jun;21:199–216. [PubMed] [Google Scholar]
- Anderson N. G., Barringer H. P., Babelay E. F., Nunley C. E., Bartkus M. J., Fisher W. D., Rankin C. T., Jr The design and operation of the B-IV zonal centrifuge system. Natl Cancer Inst Monogr. 1966 Jun;21:137–164. [PubMed] [Google Scholar]
- Barringer H. P., Anderson N. G., Nunley C. E. Design of the B-V continuous-flow centrifuge system. Natl Cancer Inst Monogr. 1966 Jun;21:191–198. [PubMed] [Google Scholar]
- CHANOCK R. M., PARROTT R. H., COOK K., ANDREWS B. E., BELL J. A., REICHELDERFER T., KAPIKIAN A. Z., MASTROTA F. M., HUEBNER R. J. Newly recognized myxoviruses from children with respiratory disease. N Engl J Med. 1958 Jan 30;258(5):207–213. doi: 10.1056/NEJM195801302580502. [DOI] [PubMed] [Google Scholar]
- CHANOCK R., ROIZMAN B., MYERS R. Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). I. Isolation, properties and characterization. Am J Hyg. 1957 Nov;66(3):281–290. doi: 10.1093/oxfordjournals.aje.a119901. [DOI] [PubMed] [Google Scholar]
- COATES H. V., CHANOCK R. M. CLINICAL SIGNIFICANCE OF RESPIRATORY SYNCYTIAL VIRUS. Postgrad Med. 1964 May;35:460–465. doi: 10.1080/00325481.1964.11695112. [DOI] [PubMed] [Google Scholar]
- Coates H. V., Alling D. W., Chanock R. M. An antigenic analysis of respiratory syncytial virus isolates by a plaque reduction neutralization test. Am J Epidemiol. 1966 Mar;83(2):299–313. doi: 10.1093/oxfordjournals.aje.a120586. [DOI] [PubMed] [Google Scholar]
- JOHNSON K. M., CHANOCK R. M., RIFKIND D., KRAVETZ H. M., KNIGHT V. Respiratory syncytial virus. IV. Correlation of virus shedding, serologic response, and illness in adult volunteers. JAMA. 1961 May 27;176:663–667. [PubMed] [Google Scholar]