Abstract
Temperature-sensitive mutants of phage α were subjected to short pulses of permissive temperature at various times during the lytic cycle. All the mutants showed an optimal response to the permissive pulse at a specific time after infection. The optimal responses of the mutants belonging to the same complementation group fell close together in the same time interval; the optimal responses of mutants contained in 20 different complementation groups were more or less uniformly scattered throughout the lytic cycle. Temperature sensitivity, therefore, seems to afford, at least in the case of phage α, an independent way of grouping the genes in an ordered sequence with respect to the steps they control.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Dove W. F. Action of the lambda chromosome. I. Control of functions late in bacteriophage development. J Mol Biol. 1966 Aug;19(1):187–201. doi: 10.1016/s0022-2836(66)80060-8. [DOI] [PubMed] [Google Scholar]
- EDLIN G. GENE REGULATION DURING BACTERIOPHAGE T4 DEVLOPMENT. I. PHENOTYPIC REVERSION OF T4 AMBER MUTANTS BY 5-FLUOROURACIL. J Mol Biol. 1965 Jun;12:363–374. doi: 10.1016/s0022-2836(65)80260-1. [DOI] [PubMed] [Google Scholar]
- Eisen H. A., Fuerst C. R., Siminovitch L., Thomas R., Lambert L., Pereira da Silva L., Jacob F. Genetics and physiology of defective lysogeny in K12 (lambda): studies of early mutants. Virology. 1966 Oct;30(2):224–241. doi: 10.1016/0042-6822(66)90098-5. [DOI] [PubMed] [Google Scholar]
- Ephrussi B, Herold J L. Studies of Eye Pigments of Drosophila II. Effect of Temperature on the Red and Brown Pigments in the Mutant Blood (w). Genetics. 1945 Jan;30(1):62–70. doi: 10.1093/genetics/30.1.62. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GEIDUSCHEK E. P., TOCCHINI-VALENTINI G. P., SARNAT M. T. ASYMMETRIC SYNTHESIS OF RNA IN VITRO: DEPENDENCE OF DNA CONTINUITY AND CONFORMATION. Proc Natl Acad Sci U S A. 1964 Aug;52:486–493. doi: 10.1073/pnas.52.2.486. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joyner A., Isaacs L. N., Echols H., Sly W. S. DNA replication and messenger RNA production after induction of wild-type lambda bacteriophage and lambda mutants. J Mol Biol. 1966 Aug;19(1):174–186. doi: 10.1016/s0022-2836(66)80059-1. [DOI] [PubMed] [Google Scholar]
- Kornberg A., Zimmerman S. B., Kornberg S. R., Josse J. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. INFLUENCE OF BACTERIOPHAGE T2 ON THE SYNTHETIC PATHWAY IN HOST CELLS. Proc Natl Acad Sci U S A. 1959 Jun;45(6):772–785. doi: 10.1073/pnas.45.6.772. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TOCCHINI-VALENTINI G. P., STODOLSKY M., AURISICCHIO A., SARNAT M., GRAZIOSI F., WEISS S. B., GEIDUSCHEK E. P. ON THE ASYMMETRY OF RNA SYNTHESIS IN VIVO. Proc Natl Acad Sci U S A. 1963 Nov;50:935–942. doi: 10.1073/pnas.50.5.935. [DOI] [PMC free article] [PubMed] [Google Scholar]