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. 1967 Oct;1(5):868–875. doi: 10.1128/jvi.1.5.868-875.1967

Attachment and Eclipse of Adenovirus

Lennart Philipson 1
PMCID: PMC375363  PMID: 5621483

Abstract

The attachment and eclipse of adenovirus have been studied with the aid of highly purified 14C-threonine and 32P-labeled adenovirus type 2 in KB cells in suspension cultures. Adenovirus particles and infectivity appear to attach at the same rate. The attachment rate appears to be highly dependent on the cell concentration and less dependent on virus concentration within the multiplicity range from 0.15 to 3 plaque-forming units per cell, probably corresponding to 4.5 to 90 particles per cell. Subsequent to attachment, 5 to 8% of the 14C label is eluted from the cell at a structure level, corresponding to free hexon. The 32P activity is rapidly associated with the cells and is converted within 20 to 30 min to 65 to 85% deoxyribonuclease-susceptible material. This process is unaffected by actinomycin and puromycin. The deoxyribonuclease-sensitive material is, however, associated with 14C label for an extended period after infection, and does not sediment as free deoxyribonucleic acid in sucrose gradients. The implications of these findings on the penetration mechanism of animal viruses are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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