Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1967 Oct;1(5):898–904. doi: 10.1128/jvi.1.5.898-904.1967

Two Loci Controlling Genetic Cellular Resistance to Avian Leukosis-Sarcoma Viruses

Lyman B Crittenden 1, Howard A Stone 1, Richard H Reamer 1, William Okazaki 1
PMCID: PMC375367  PMID: 4316238

Abstract

Female chickens known to be heterozygous for resistance to subgroups A and B of the avian leukosis-sarcoma viruses were mated to males known to be homozygously resistant to both. The progeny were assayed both on the chorioallantoic membrane (CAM) and in tissue culture for resistance to representative viruses of the A, B, and tentatively defined C subgroups. Segregation ratios of resistance to A and B subgroup viruses agreed with the previously suggested hypothesis of single-autosomal-recessive genes controlling resistance to each subgroup. Mixed infection on the CAM and replicate plate infection in tissue culture with subgroup A and B viruses showed that resistance to the A and B subgroups was inherited independently. Assays with viruses tentatively classified as subgroup C indicated that they were largely composed of a mixture of subgroup A and B viruses or of particles possessing the host range specificity of both. However, virus stocks of the subgroup C category, as well as some stocks classified as subgroup B, produced small numbers of pocks or foci on individuals known to be resistant to subgroup A and B viruses. It is suggested that these Rous sarcoma virus stocks carry between 1 and 10% of a true subgroup C virus.

Full text

PDF
901

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Crittenden L. B., Okazaki W. Genetic influence of the Rs locus on susceptibility to avian tumor viruses. I. Neoplasms induced by RPL-12 and three strains of Rous sarcoma virus. J Natl Cancer Inst. 1965 Nov;35(5):857–863. [PubMed] [Google Scholar]
  2. Crittenden L. B., Okazaki W. Genetic influence of the Rs locus on susceptibility to avian tumor viruses. II. Rous sarcoma virus antibody production after strain RPL12 virus inoculation. J Natl Cancer Inst. 1966 Feb;36(2):299–303. [PubMed] [Google Scholar]
  3. GROUPE V., DUNKEL V. C., MANAKER R. A. Improved pock counting method for the titration of Rous sarcoma virus in embryonated eggs. J Bacteriol. 1957 Sep;74(3):409–410. doi: 10.1128/jb.74.3.409-410.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hanafusa H., Hanafusa T. Determining factor in the capacity of Rous sarcoma virus to induce tumors in mammals. Proc Natl Acad Sci U S A. 1966 Mar;55(3):532–538. doi: 10.1073/pnas.55.3.532. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ishizaki R., Vogt P. K. Immunological relationships among envelope antigens of avian tumor viruses. Virology. 1966 Nov;30(3):375–387. doi: 10.1016/0042-6822(66)90116-4. [DOI] [PubMed] [Google Scholar]
  6. PAYNE L. N., BIGGS P. M. DIFFERENCES BETWEEN HIGHLY INBRED LINES OF CHICKENS IN THE RESPONSE TO ROUS SARCOMA VIRUS OF THE CHORIOALLANTOIC MEMBRANE AND OF EMBRYONIC CELLS IN TISSUE CULTURE. Virology. 1964 Dec;24:610–616. doi: 10.1016/0042-6822(64)90215-6. [DOI] [PubMed] [Google Scholar]
  7. PURCHASE H. G., OKAZAKI W. MORPHOLOGY OF FOCI PRODUCED BY STANDARD PREPARATION OF ROUS SARCOMA VIRUS. J Natl Cancer Inst. 1964 Mar;32:579–586. [PubMed] [Google Scholar]
  8. Payne L. N., Biggs P. M. Genetic basis of cellular susceptibility to the Schmidt-Ruppin and Harris strains of Rous sarcoma virus. Virology. 1966 Jun;29(2):190–198. doi: 10.1016/0042-6822(66)90025-0. [DOI] [PubMed] [Google Scholar]
  9. RUBIN H. GENETIC CONTROL OF CELLULAR SUSCEPTIBILITY TO PSEUDOTYPES OF ROUS SARCOMA VIRUS. Virology. 1965 Jun;26:270–276. doi: 10.1016/0042-6822(65)90274-6. [DOI] [PubMed] [Google Scholar]
  10. SARMA P. S., HUEBNER R. J., ARMSTRONG D. A SIMPLIFIED TISSUE CULTURE TUBE NEUTRALIZATION TEST FOR ROUS SARCOMA VIRUS ANTIBODIES. Proc Soc Exp Biol Med. 1964 Feb;115:481–486. doi: 10.3181/00379727-115-28947. [DOI] [PubMed] [Google Scholar]
  11. TEMIN H. M., RUBIN H. Characteristics of an assay for Rous sarcoma virus and Rous sarcoma cells in tissue culture. Virology. 1958 Dec;6(3):669–688. doi: 10.1016/0042-6822(58)90114-4. [DOI] [PubMed] [Google Scholar]
  12. Vogt P. K., Ishizaki R. Patterns of viral interference in the avian leukosis and sarcoma complex. Virology. 1966 Nov;30(3):368–374. doi: 10.1016/0042-6822(66)90115-2. [DOI] [PubMed] [Google Scholar]
  13. Vogt P. K., Ishizaki R. Reciprocal patterns of genetic resistance to avian tumor viruses in two lines of chickens. Virology. 1965 Aug;26(4):664–672. doi: 10.1016/0042-6822(65)90329-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES