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. 1967 Oct;1(5):1019–1027. doi: 10.1128/jvi.1.5.1019-1027.1967

Morphogenesis of Avian Infectious Bronchitis Virus and a Related Human Virus (Strain 229E)

Walter B Becker a,1, Kenneth McIntosh a, Jane H Dees a, Robert M Chanock a
PMCID: PMC375381  PMID: 5630226

Abstract

Avian infectious bronchitis virus (IBV) and strain 229E, a virus recently recovered from patients with colds, have been shown to possess a similar distinctive morphology in negatively stained preparations. An electron microscopic study of the morphogenesis of IBV in the chorioallantoic membrane and of strain 229E in WI-38 cells was performed. In infected cells, round electron-dense particles 82 mμ in diameter were observed to form by a process of budding from membranes of the endoplasmic reticulum and cytoplasmic vesicles. The particles in IBV-infected cells were similar in size and shape to those in strain 229E-infected cells but showed certain differences in internal structure. The evidence that the particles represent virions and the implications of these findings in the classification of this virus group are discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Almeida J. D., Tyrrell D. A. The morphology of three previously uncharacterized human respiratory viruses that grow in organ culture. J Gen Virol. 1967 Apr;1(2):175–178. doi: 10.1099/0022-1317-1-2-175. [DOI] [PubMed] [Google Scholar]
  2. BERRY D. M., CRUICKSHANK J. G., CHU H. P., WELLS R. J. THE STRUCTURE OF INFECTIOUS BRONCHITIS VIRUS. Virology. 1964 Jul;23:403–407. doi: 10.1016/0042-6822(64)90263-6. [DOI] [PubMed] [Google Scholar]
  3. BONAR R. A., HEINE U., BEARD D., BEARD J. W. Virus of avian myeloblastosis (BAI strain A). 23. Morphology of virus and comparison with strain R (erythroblastosis). J Natl Cancer Inst. 1963 May;30:949–997. [PubMed] [Google Scholar]
  4. DAVID-FERREIRA J. F., MANAKER R. A. AN ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF A MOUSE HEPATITIS VIRUS IN TISSUE CULTURE CELLS. J Cell Biol. 1965 Jan;24:57–78. doi: 10.1083/jcb.24.1.57. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DOMERMUTH C. H., EDWARDS O. F. An electron microscope study of chorioallantoic membrane infected with the virus of avian infectious bronchitis. J Infect Dis. 1957 Jan-Feb;100(1):74–81. doi: 10.1093/infdis/100.1.74. [DOI] [PubMed] [Google Scholar]
  6. GLEDHILL A. W., ANDREWES C. H. A hepatitis virus of mice. Br J Exp Pathol. 1951 Dec;32(6):559–568. [PMC free article] [PubMed] [Google Scholar]
  7. Hamre D., Kindig D. A., Mann J. Growth and intracellular development of a new respiratory virus. J Virol. 1967 Aug;1(4):810–816. doi: 10.1128/jvi.1.4.810-816.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hamre D., Procknow J. J. A new virus isolated from the human respiratory tract. Proc Soc Exp Biol Med. 1966 Jan;121(1):190–193. doi: 10.3181/00379727-121-30734. [DOI] [PubMed] [Google Scholar]
  9. MALLUCCI L. OBSERVATIONS ON THE GROWTH OF MOUSE HEPATITIS VIRUS (MHV-3) IN MOUSE MACROPHAGES. Virology. 1965 Jan;25:30–37. doi: 10.1016/0042-6822(65)90248-5. [DOI] [PubMed] [Google Scholar]
  10. McIntosh K., Dees J. H., Becker W. B., Kapikian A. Z., Chanock R. M. Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc Natl Acad Sci U S A. 1967 Apr;57(4):933–940. doi: 10.1073/pnas.57.4.933. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. SARMA P. S., TURNER H. C., HUEBNER R. J. AN AVIAN LEUCOSIS GROUP-SPECIFIC COMPLEMENT FIXATION REACTION. APPLICATION FOR THE DETECTION AND ASSAY OF NON-CYTOPATHOGENIC LEUCOSIS VIRUSES. Virology. 1964 Jul;23:313–321. doi: 10.1016/0042-6822(64)90253-3. [DOI] [PubMed] [Google Scholar]
  15. TYRRELL D. A., BYNOE M. L. CULTIVATION OF A NOVEL TYPE OF COMMON-COLD VIRUS IN ORGAN CULTURES. Br Med J. 1965 Jun 5;1(5448):1467–1470. doi: 10.1136/bmj.1.5448.1467. [DOI] [PMC free article] [PubMed] [Google Scholar]

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