Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1967 Dec;1(6):1193–1202. doi: 10.1128/jvi.1.6.1193-1202.1967

Molecular Recombination in T4 Bacteriophage Deoxyribonucleic Acid II. Single-Strand Breaks and Exposure of Uncomplemented Areas as a Prerequisite for Recombination

Andrzej W Kozinski 1, Zoya Z Felgenhauer 1
PMCID: PMC375409  PMID: 4912244

Abstract

Deoxyribonucleic acid (DNA) from several “DNA-deficient” amber mutants was observed to be either nicked (amber 22, 82, 122, and wild type) or cut (amber 453) after injection into a nonpermissive host. This effect was inhibited by chloramphenicol (CM), indicating that it is due to phage-induced enzymes. Although most of the mutants tested for replication in a density-label system were in fact DNA-deficient (amber 22, 82, 122), one (amber 81) was found to replicate almost identically to the wild type, and another (amber 453) was found to assume a hybrid density only. The hybrid moiety was less than, or equal to, one phage equivalent length, and was more efficiently extracted from infected bacteria than was similarly replicated DNA from wild-type phage. Interparental recombination between heavy and light parental DNA was observed for amber 82, 122, and wild type, but was not observed for amber 453; it was inhibited by CM. In contrast to amber 82 and wild type, the amber 453 intracellular DNA does not have single-strand regions, Because amber 453, unlike amber 82 and wild type T4, does not recombine, nicking and exposure of single-strand regions is postulated to be a prerequisite for recombination.

Full text

PDF
1198

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anraku N., Tomizawa J. Molecular mechanisms of genetic recombination of bacteriophage. V. Two kinds of joining of parental DNA molecules. J Mol Biol. 1965 Jul;12(3):805–815. doi: 10.1016/s0022-2836(65)80329-1. [DOI] [PubMed] [Google Scholar]
  2. BERNS K. I., THOMAS C. A., Jr ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE. J Mol Biol. 1965 Mar;11:476–490. doi: 10.1016/s0022-2836(65)80004-3. [DOI] [PubMed] [Google Scholar]
  3. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  4. KOZINSKI A. W., KOZINSKI P. B. Fragmentary transfer of P32-labeled parental DNA to progeny phage. II. The average size of the transferred parental fragment. Two-cycletransfer. Repair of the polynucleotide chain after fragmentation. Virology. 1963 Jun;20:213–229. doi: 10.1016/0042-6822(63)90109-0. [DOI] [PubMed] [Google Scholar]
  5. KOZINSKI A. W., KOZINSKI P. B. REPLICATIVE FRAGMENTATION IN T4 BACTERIOPHAGE DNA. II. BIPARENTAL MOLECULAR RECOMBINATION. Proc Natl Acad Sci U S A. 1964 Aug;52:211–218. doi: 10.1073/pnas.52.2.211. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. KOZINSKI A. W., SZYBALSKI W. Dispersive transfer of the parental DNA molecule to the progeny of phage phiX-174. Virology. 1959 Oct;9:260–274. doi: 10.1016/0042-6822(59)90119-9. [DOI] [PubMed] [Google Scholar]
  7. Kozinski A. W., Kozinski P. B. Early intracellular events in the replication T4 phage DNA. II. Partially replicated DNA. Proc Natl Acad Sci U S A. 1965 Aug;54(2):634–640. doi: 10.1073/pnas.54.2.634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kozinski A. W., Kozinski P. B. Early intracellular events in the replication of T4 phage DNA. 3. Random utilization of phage-coded enzymes by simultaneously infecting phage. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1705–1711. doi: 10.1073/pnas.57.6.1705. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kozinski A. W., Kozinski P. B., James R. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. I. Tertiary structure of early replicative and recombining deoxyribonucleic acid. J Virol. 1967 Aug;1(4):758–770. doi: 10.1128/jvi.1.4.758-770.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kozinski A. W., Lin T. H. Early intracellular events in the replication of T4 phage DNA. I. Complex formation of replicative DNA. Proc Natl Acad Sci U S A. 1965 Jul;54(1):273–278. doi: 10.1073/pnas.54.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kozinski A. W., Shahn E. A new ultracentrifuge rotor for cesium chloride gradient equilibrium runs. Virology. 1966 Feb;28(2):346–348. doi: 10.1016/0042-6822(66)90159-0. [DOI] [PubMed] [Google Scholar]
  12. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  13. Tomizawa J. I., Anraku N., Iwama Y. Molecular mechanisms of genetic recombination in bacteriophage. VI. A mutant defective in the joining of DNA molecules. J Mol Biol. 1966 Nov 14;21(2):247–253. doi: 10.1016/0022-2836(66)90095-7. [DOI] [PubMed] [Google Scholar]
  14. WIBERG J. S., DIRKSEN M. L., EPSTEIN R. H., LURIA S. E., BUCHANAN J. M. Early enzyme synthesis and its control in E. coli infected with some amber mutants of bacteriophage T4. Proc Natl Acad Sci U S A. 1962 Feb;48:293–302. doi: 10.1073/pnas.48.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weiss B., Richardson C. C. Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage. Proc Natl Acad Sci U S A. 1967 Apr;57(4):1021–1028. doi: 10.1073/pnas.57.4.1021. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES