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. 1967 Dec;1(6):1207–1216. doi: 10.1128/jvi.1.6.1207-1216.1967

Purification of Large Quantities of Influenza Virus by Density Gradient Centrifugation

C B Reimer 1,2,1, R S Baker 1,2, R M vanFrank 1,2, T E Newlin 1,2, G B Cline 1,2,2, N G Anderson 1,2
PMCID: PMC375411  PMID: 5621490

Abstract

New zonal centrifuges can conveniently process as much as five orders of magnitude (105) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding versions may be used in series with ancillary purification procedures. Here we have studied the combined process: absorption and elution of influenza virus with barium sulfate followed by concentration and isopycnic banding of the virus in a buffered sucrose gradient. Kilogram quantities of impurity have been rapidly separated from grams of purified virus, which have been conveniently concentrated several hundred-fold by the purification process. Experimental vaccines made by these procedures are being evaluated.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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