Skip to main content
Journal of Virology logoLink to Journal of Virology
. 1967 Dec;1(6):1224–1226. doi: 10.1128/jvi.1.6.1224-1226.1967

Reproducible Plaquing System for Rabies, Lymphocytic Choriomeningitis, and Other Ribonucleic Acid Viruses in BHK-21 / 13S Agarose Suspensions

W D Sedwick 1, T J Wiktor 1
PMCID: PMC375413  PMID: 4987176

Abstract

BHK21/13S-agarose suspension cultures provide an excellent plaquing system for rabies and lymphocytic choriomeningitis viruses. The system has facilitated accurate and reproducible assay of serum neutralization and cloning of these viruses. Rubella, reo, and Kern Canyon viruses may also be assayed by this technique.

Full text

PDF
1226

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Diamond L. Two spontaneously transformed cell lines derived from the same hamster embryo culture. Int J Cancer. 1967 Mar 15;2(2):143–152. doi: 10.1002/ijc.2910020209. [DOI] [PubMed] [Google Scholar]
  2. FERNANDES M. V., WIKTOR T. J., KOPROWSKI H. MECHANISM OF THE CYTOPATHIC EFFECT OF RABIES VIRUS IN TISSUE CULTURE. Virology. 1963 Sep;21:128–131. doi: 10.1016/0042-6822(63)90312-x. [DOI] [PubMed] [Google Scholar]
  3. KANDA Y., MELNICK J. L. In vitro differentiation of virulent and attenuated polioviruses by their growth characteristics on MS cells. J Exp Med. 1959 Jan 1;109(1):9–24. doi: 10.1084/jem.109.1.9. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. MACPHERSON I., STOKER M. Polyoma transformation of hamster cell clones--an investigation of genetic factors affecting cell competence. Virology. 1962 Feb;16:147–151. doi: 10.1016/0042-6822(62)90290-8. [DOI] [PubMed] [Google Scholar]
  5. Vaheri A., Sedwick W. D., Plotkin S. A., Maes R. Cytopathic effect of rubella virus in RHK21 cells and growth to high titers in suspension culture. Virology. 1965 Oct;27(2):239–241. doi: 10.1016/0042-6822(65)90170-4. [DOI] [PubMed] [Google Scholar]
  6. WIKTOR T. J., FERNANDES M. V., KOPROWSKI H. CULTIVATION OF RABIES VIRUS IN HUMAN DIPLOID CELL STRAIN WI-38. J Immunol. 1964 Sep;93:353–366. [PubMed] [Google Scholar]
  7. Wiktor T. J., Kaplan M. M., Koprowski H. Rabies and lymphocytic choriomeningitis virus (LCMV) infection of tissue culture; enhancing effect of LCMV. Ann Med Exp Biol Fenn. 1966;44(2):290–296. [PubMed] [Google Scholar]
  8. Zgorniak-Nowosielska I., Sedwick W. D., Hummeler K., Koprowski H. New assay procedure for separation of mycoplasmas from virus pools and tissue culture systems. J Virol. 1967 Dec;1(6):1227–1237. doi: 10.1128/jvi.1.6.1227-1237.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES