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. 1968 Oct;2(10):1096–1101. doi: 10.1128/jvi.2.10.1096-1101.1968

Evidence for a New Endonuclease Synthesized by λ Bacteriophage

Robert C Shuster a,1, Arthur Weissbach a,2
PMCID: PMC375441  PMID: 5723713

Abstract

Infection of nonlysogenic Escherichia coli CR34(S) (Thy−) with bacteriophage λ CI857 resulted in the formation of twisted circular double-stranded phage deoxyribonucleic acid (DNA; species I). When such infected bacteria were incubated in the absence of thymine, there was a significant decrease in the amount of species I DNA after 60 min of incubation. A similar loss of species I λ DNA during incubation in a thymine-deficient medium was also observed after infection of the endonuclease I-deficient strain, E. coli 1100(S) (Thy−). This destruction of twisted, circular λ DNA in thymine-deprived cells did not occur in the presence of chloramphenicol nor in lysogenic E. coli CR34 carrying a noninducible λ prophage. It is therefore concluded that the endonuclease which attacks this circular configuration of λ DNA is newly synthesized after infection and is directed by the phage chromosome.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BROOKS K. STUDIES IN THE PHYSIOLOGICAL GENETICS OF SOME SUPPRESSOR-SENSITIVE MUTANTS OF BACTERIOPHAGE LAMBDA. Virology. 1965 Jul;26:489–499. doi: 10.1016/0042-6822(65)90011-5. [DOI] [PubMed] [Google Scholar]
  2. Bode V. C., Kaiser A. D. Changes in the structure and activity of lambda DNA in a superinfected immune bacterium. J Mol Biol. 1965 Dec;14(2):399–417. doi: 10.1016/s0022-2836(65)80190-5. [DOI] [PubMed] [Google Scholar]
  3. Eisen H. A., Fuerst C. R., Siminovitch L., Thomas R., Lambert L., Pereira da Silva L., Jacob F. Genetics and physiology of defective lysogeny in K12 (lambda): studies of early mutants. Virology. 1966 Oct;30(2):224–241. doi: 10.1016/0042-6822(66)90098-5. [DOI] [PubMed] [Google Scholar]
  4. Gingery R., Echols H. Mutants of bacteriophage lambda unable to integrate into the host chromosome. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1507–1514. doi: 10.1073/pnas.58.4.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gottesman M. E., Yarmolinsky M. B. Integration-negative mutants of bacteriophage lambda. J Mol Biol. 1968 Feb 14;31(3):487–505. doi: 10.1016/0022-2836(68)90423-3. [DOI] [PubMed] [Google Scholar]
  6. Joyner A., Isaacs L. N., Echols H., Sly W. S. DNA replication and messenger RNA production after induction of wild-type lambda bacteriophage and lambda mutants. J Mol Biol. 1966 Aug;19(1):174–186. doi: 10.1016/s0022-2836(66)80059-1. [DOI] [PubMed] [Google Scholar]
  7. KORN D. STUDY OF THE DEVELOPMENT OF RESISTANCE TO LAMBDAIND-REPRESSION DURING THYMINELESS INDUCTION OF ESCHERICHIA COLI K12(LAMBDA)THY-. Virology. 1964 Dec;24:570–577. doi: 10.1016/0042-6822(64)90209-0. [DOI] [PubMed] [Google Scholar]
  8. Makover S. A preferred origin for the replication of lambda DNA. Proc Natl Acad Sci U S A. 1968 Apr;59(4):1345–1348. doi: 10.1073/pnas.59.4.1345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. STACEY K. A., SIMSON E. IMPROVED METHOD FOR THE ISOLATION OF THYMINE-REQUIRING MUTANTS OF ESCHERICHIA COLI. J Bacteriol. 1965 Aug;90:554–555. doi: 10.1128/jb.90.2.554-555.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. SUSSMAN R., JACOB F. [On a thermosensitive repression system in the Escherichia coli lambda bacteriophage]. C R Hebd Seances Acad Sci. 1962 Feb 19;254:1517–1519. [PubMed] [Google Scholar]
  11. Salzman L. A., Weissbach A. Formation of intermediates in the replication of phage lambda DNA. J Mol Biol. 1967 Aug 28;28(1):53–70. doi: 10.1016/s0022-2836(67)80077-9. [DOI] [PubMed] [Google Scholar]
  12. Salzman L. A., Weissbach A. Studies on the formation of circular lambda DNA. Virology. 1967 Jan;31(1):70–77. doi: 10.1016/0042-6822(67)90009-8. [DOI] [PubMed] [Google Scholar]
  13. Weissbach A., Lipton A., Lisio A. Intracellular forms of lambda deoxyribonucleic acid in Escherichia coli infected with clear or virulent mutants of bacteriophage lambda. J Bacteriol. 1966 Apr;91(4):1489–1493. doi: 10.1128/jb.91.4.1489-1493.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. YOUNG E. T., 2nd, SINSHEIMER R. L. NOVEL INTRA-CELLULAR FORMS OF LAMBDA DNA. J Mol Biol. 1964 Dec;10:562–564. doi: 10.1016/s0022-2836(64)80080-2. [DOI] [PubMed] [Google Scholar]
  15. Young E. T., 2nd, Sinsheimer R. L. Vegetative bacteriophage lambda-DNA. II. Physical characterization and replication. J Mol Biol. 1967 Nov 28;30(1):165–200. doi: 10.1016/0022-2836(67)90251-3. [DOI] [PubMed] [Google Scholar]

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