Abstract
Cycloheximide, at a concentration of 10 μg/ml, rapidly blocked protein synthesis in L cells infected with reovirus. When the drug was added before 5 hr postinfection, synthesis of both single- and double-stranded varieties of virus-specific ribonucleic acid (RNA), which normally commences between 6 and 7 hr after infection, was blocked. When the cycloheximide was added at 9 hr after infection, uptake of uridine-H3 into RNA, for the succeeding 6 hr at least, was similar to that of an infected culture without the drug. This latter uptake of uridine-H3 in the presence of cycloheximide was largely into single-stranded RNA, since double-stranded RNA synthesis was rapidly and markedly inhibited by the cycloheximide. Single-stranded RNA formed in the presence of cycloheximide was found not to be a precursor of viral progeny, double-stranded RNA. Synthesis of double-stranded RNA in the infected cell probably requires prior synthesis of a new protein, which has a rapid rate of turnover. The possibility that formation of single-stranded RNA is preceded by synthesis of a second new protein is discussed.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ENNIS H. L., LUBIN M. CYCLOHEXIMIDE: ASPECTS OF INHIBITION OF PROTEIN SYNTHESIS IN MAMMALIAN CELLS. Science. 1964 Dec 11;146(3650):1474–1476. doi: 10.1126/science.146.3650.1474. [DOI] [PubMed] [Google Scholar]
- Kudo H., Graham A. F. Selective inhibition of reovirus induced RNA in L cells. Biochem Biophys Res Commun. 1966 Jul 20;24(2):150–155. doi: 10.1016/0006-291x(66)90711-x. [DOI] [PubMed] [Google Scholar]
- Kudo H., Graham A. F. Synthesis of reovirus ribonucleic acid in L cells. J Bacteriol. 1965 Oct;90(4):936–945. doi: 10.1128/jb.90.4.936-945.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Loh P. C., Soergel M. Growth characteristics of reovirus type 2: actinomycin D and the synthesis of viral RNA. Proc Natl Acad Sci U S A. 1965 Sep;54(3):857–863. doi: 10.1073/pnas.54.3.857. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NATHANS D. INHIBITION OF PROTEIN SYNTHESIS BY PUROMYCIN. Fed Proc. 1964 Sep-Oct;23:984–989. [PubMed] [Google Scholar]
- Prevec L., Graham A. F. Reovirus-specific polyribosomes in infected L-cells. Science. 1966 Oct 28;154(3748):522–523. [PubMed] [Google Scholar]
- Stanners C. P. The effect of cycloheximide on polyribosomes from hamster cells. Biochem Biophys Res Commun. 1966 Sep 8;24(5):758–764. doi: 10.1016/0006-291x(66)90390-1. [DOI] [PubMed] [Google Scholar]
- WETTSTEIN F. O., NOLL H., PENMAN S. EFFECT OF CYCLOHEXIMIDE ON RIBOSOMAL AGGREGATES ENGAGED IN PROTEIN SYNTHESIS IN VITRO. Biochim Biophys Acta. 1964 Jul 22;87:525–528. doi: 10.1016/0926-6550(64)90131-8. [DOI] [PubMed] [Google Scholar]