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. 1967 Feb;1(1):86–91. doi: 10.1128/jvi.1.1.86-91.1967

Deoxyribonucleic Acid Replication and Expression of Early and Late Bacteriophage Functions in Bacillus subtilis

Jacques J Pène 1, Julius Marmur 1
PMCID: PMC375507  PMID: 4990039

Abstract

The role of deoxyribonucleic acid (DNA) replication in the control of the synthesis of deoxycytidylate (dCMP) deaminase and lysozyme in Bacillus subtilis infected with bacteriophage 2C has been studied. These phage-induced enzymes are synthesized at different times during the latent period. It was shown by actinomycin inhibition that the formation of the late enzyme (lysozyme) required messenger ribonucleic acid (mRNA) synthesized de novo after the initiation of translation of mRNA which specifies the early function (dCMP deaminase). The inhibition of phage DNA synthesis by mitomycin C prevented the synthesis of lysozyme only when added before the onset of phage DNA replication, but it did not affect the synthesis or action of dCMP deaminase when added at any time during the latent period. Treatment of infected cells with mitomycin C after phage DNA synthesis had reached 8 to 10% of its maximal rate resulted in the production of normal amounts of lysozyme. These observations suggest that mRNA specifying early enzymes can be transcribed from parental (and probably also from progeny) DNA, whereas late functional messengers can be transcribed only after the formation of progeny DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bautz E. K., Kasai T., Reilly E., Bautz F. A. Gene-specific mRNA. II. Regulation of mRNA synthesis in E. coli after infection with bacteriophage T4. Proc Natl Acad Sci U S A. 1966 May;55(5):1081–1088. doi: 10.1073/pnas.55.5.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CHAMPE S. P. BACTERIOPHAGE REPRODUCTION. Annu Rev Microbiol. 1963;17:87–114. doi: 10.1146/annurev.mi.17.100163.000511. [DOI] [PubMed] [Google Scholar]
  3. Dove W. F. Action of the lambda chromosome. I. Control of functions late in bacteriophage development. J Mol Biol. 1966 Aug;19(1):187–201. doi: 10.1016/s0022-2836(66)80060-8. [DOI] [PubMed] [Google Scholar]
  4. EDLIN G. GENE REGULATION DURING BACTERIOPHAGE T4 DEVLOPMENT. I. PHENOTYPIC REVERSION OF T4 AMBER MUTANTS BY 5-FLUOROURACIL. J Mol Biol. 1965 Jun;12:363–374. doi: 10.1016/s0022-2836(65)80260-1. [DOI] [PubMed] [Google Scholar]
  5. Frankel F. R. Studies on the nature of replicating DNA in T4-infected Escherichia coli. J Mol Biol. 1966 Jun;18(1):127–143. doi: 10.1016/s0022-2836(66)80081-5. [DOI] [PubMed] [Google Scholar]
  6. Geiduschek E. P., Snyder L., Colvill A. J., Sarnat M. Selective synthesis of T-even bacteriophage early messenger in vitro. J Mol Biol. 1966 Aug;19(2):541–547. doi: 10.1016/s0022-2836(66)80021-9. [DOI] [PubMed] [Google Scholar]
  7. IYER V. N., SZYBALSKI W. MITOMYCINS AND PORFIROMYCIN: CHEMICAL MECHANISM OF ACTIVATION AND CROSS-LINKING OF DNA. Science. 1964 Jul 3;145(3627):55–58. doi: 10.1126/science.145.3627.55. [DOI] [PubMed] [Google Scholar]
  8. Kornberg A., Zimmerman S. B., Kornberg S. R., Josse J. ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEIC ACID. INFLUENCE OF BACTERIOPHAGE T2 ON THE SYNTHETIC PATHWAY IN HOST CELLS. Proc Natl Acad Sci U S A. 1959 Jun;45(6):772–785. doi: 10.1073/pnas.45.6.772. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LURIA S. E. Genetics of bacteriophage. Annu Rev Microbiol. 1962;16:205–240. doi: 10.1146/annurev.mi.16.100162.001225. [DOI] [PubMed] [Google Scholar]
  10. MALEY F., MALEY G. F. Nucleotide interconversions. II. Elevation of deoxycytidylate deaminase and thymidylate synthetase in regenerating rat liver. J Biol Chem. 1960 Oct;235:2968–2970. [PubMed] [Google Scholar]
  11. Protass J. J., Korn D. Inhibition of lysozyme synthesis by actinomycin D in bacteriophage T4-infected cells of Escherichia coli. Proc Natl Acad Sci U S A. 1966 Apr;55(4):832–835. doi: 10.1073/pnas.55.4.832. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Vinograd J., Lebowitz J. Physical and topological properties of circular DNA. J Gen Physiol. 1966 Jul;49(6):103–125. doi: 10.1085/jgp.49.6.103. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WIBERG J. S., DIRKSEN M. L., EPSTEIN R. H., LURIA S. E., BUCHANAN J. M. Early enzyme synthesis and its control in E. coli infected with some amber mutants of bacteriophage T4. Proc Natl Acad Sci U S A. 1962 Feb;48:293–302. doi: 10.1073/pnas.48.2.293. [DOI] [PMC free article] [PubMed] [Google Scholar]

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