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. 1968 Feb;2(2):149–154. doi: 10.1128/jvi.2.2.149-154.1968

Biophysical Studies on Rhinovirus and Poliovirus

I. Morphology of Viral Ribonucleoprotein

Sandy McGregor 1, Heather D Mayor 1
PMCID: PMC375591  PMID: 4316016

Abstract

An explanation has been sought for the high buoyant density of rhinoviruses, which are classified as acid-sensitive picornaviruses. Heat degradation of purified preparations of rhinovirus type 1B and poliovirus type LSc leads to the extrusion of ribonucleoprotein strands. Contour lengths of these strands were measured by electron microscopy, and the molecular weights of rhinovirus and poliovirus ribonucleic acid (RNA) were determined. Values of 2 × 106 and 4 × 106 daltons were obtained for the molecular weight of poliovirus and rhinovirus RNA, respectively. This additional nucleic acid in the rhinovirion probably accounts for the increased density and may be related to the acid sensitivity of the rhinovirus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERER F. A., RESTLE H. UNTERSUCHUNGEN UEBER EIN ATTENUIERTES POLIOMYELITIS-VIRUS TYP II, REINDARSTELLUNG UND PHYSIKALISCH-CHEMISCHE EIGENSCHAFTEN DES VIRUS. Z Naturforsch B. 1964 Nov;19:1026–1031. [PubMed] [Google Scholar]
  2. BACHRACH H. L. FOOT-AND-MOUTH DISEASE VIRUS: STRUCTURE AND MECHANISM OF DEGRADATION AS DEDUCED FROM ABSORBANCE-TEMPERATURE RELATIONSHIPS. J Mol Biol. 1964 Mar;8:348–358. doi: 10.1016/s0022-2836(64)80198-4. [DOI] [PubMed] [Google Scholar]
  3. BRENNER S., HORNE R. W. A negative staining method for high resolution electron microscopy of viruses. Biochim Biophys Acta. 1959 Jul;34:103–110. doi: 10.1016/0006-3002(59)90237-9. [DOI] [PubMed] [Google Scholar]
  4. Bresnick E., Sage J., Lanclos K. Ribonuclease activity in hepatic nuclei during development. Biochim Biophys Acta. 1966 Mar 21;114(3):631–633. doi: 10.1016/0005-2787(66)90112-2. [DOI] [PubMed] [Google Scholar]
  5. Chapple P. J., Harris W. J. Biophysical studies of a rhinovirus. Ultracentrifugation and electron microscopy. Nature. 1966 Feb 19;209(5025):790–792. doi: 10.1038/209790a0. [DOI] [PubMed] [Google Scholar]
  6. Dans P. E., Forsyth B. R., Chanock R. M. Density of infectious virus and complement-fixing antigens of two rhinovirus strains. J Bacteriol. 1966 Apr;91(4):1605–1611. doi: 10.1128/jb.91.4.1605-1611.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HOYER B. H., BOLTON E. T., RITTER D. B., RIBI E. Simple method for preparation of purified radioactive poliovirus particles: electron micrograph. Virology. 1959 Apr;7(4):462–464. doi: 10.1016/0042-6822(59)90074-1. [DOI] [PubMed] [Google Scholar]
  8. HUMMELER K., ANDERSON T. F., BROWN R. A. Identification of poliovirus particles of different antigenicity by specific agglutination as seen in the electron microscope. Virology. 1962 Jan;16:84–90. doi: 10.1016/0042-6822(62)90205-2. [DOI] [PubMed] [Google Scholar]
  9. Jamison R. M., Mayor H. D. Comparative study of seven picornaviruses of man. J Bacteriol. 1966 May;91(5):1971–1976. doi: 10.1128/jb.91.5.1971-1976.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KOCH G. Influence of assay conditions on infectivity of heated poliovirus. Virology. 1960 Dec;12:601–603. doi: 10.1016/0042-6822(60)90183-5. [DOI] [PubMed] [Google Scholar]
  11. Lang D., Bujard H., Wolff B., Russell D. Electron microscopy of size and shape of viral DNA in solutions of different ionic strengths. J Mol Biol. 1967 Jan 28;23(2):163–181. doi: 10.1016/s0022-2836(67)80024-x. [DOI] [PubMed] [Google Scholar]
  12. MACLEAN E. C., HALL C. E. Studies on bacteriophage phi-X174 and its DNA by electron microscopy. J Mol Biol. 1962 Mar;4:173–178. doi: 10.1016/s0022-2836(62)80049-7. [DOI] [PubMed] [Google Scholar]
  13. McGregor S., Phillips C. A., Mayor H. D. Purification and biophysical properties of rhinoviruses. Proc Soc Exp Biol Med. 1966 May;122(1):118–121. doi: 10.3181/00379727-122-31068. [DOI] [PubMed] [Google Scholar]
  14. Mitra S., Enger M. D., Kaesberg P. PHYSICAL AND CHEMICAL PROPERTIES OF RNA FROM THE BACTERIAL VIRUS R17. Proc Natl Acad Sci U S A. 1963 Jul;50(1):68–75. doi: 10.1073/pnas.50.1.68. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. NORMAN A., VEOMETT R. C. Heatinactivation of poliovirus ribonucleic acid. Virology. 1960 Sep;12:136–139. doi: 10.1016/0042-6822(60)90158-6. [DOI] [PubMed] [Google Scholar]
  16. ROLFE U., SINSHEIMER R. L. ANTIGENS OF BACTERIOPHAGE PHI-X174. J Immunol. 1965 Jan;94:18–21. [PubMed] [Google Scholar]
  17. SCHAFFER F. L., FROMMHAGEN L. H. SIMILARITIES OF BIOPHYSICAL PROPERTIES OF SEVERAL HUMAN ENTEROVIRUSES AS SHOWN BY DENSITY GRADIENT ULTRACENTRIFUGATION OF MIXTURES OF THE VIRUSES. Virology. 1965 Apr;25:662–664. doi: 10.1016/0042-6822(65)90095-4. [DOI] [PubMed] [Google Scholar]
  18. SCHAFFER F. L., SCHWERDT C. E. Purification of poliomyelitis viruses propagated in tissue culture. Virology. 1956 Oct;2(5):665–678. doi: 10.1016/0042-6822(56)90046-0. [DOI] [PubMed] [Google Scholar]
  19. SCHARFF M. D., MAIZEL J. V., Jr, LEVINTOW L. PHYSICAL AND IMMUNOLOGICAL PROPERTIES OF A SOLUBLE PRECURSOR OF THE POLIOVIRUS CAPSID. Proc Natl Acad Sci U S A. 1964 Feb;51:329–337. doi: 10.1073/pnas.51.2.329. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. SCHWERDT C. E., SCHAFFER F. L. Some physical and chemical properties of purified poliomyelitis virus preparations. Ann N Y Acad Sci. 1955 Sep 27;61(4):740-50; discussion, 750-3. doi: 10.1111/j.1749-6632.1955.tb42530.x. [DOI] [PubMed] [Google Scholar]
  21. SINHA N. K., FUJIMURA R. K., KAESBERG P. RIBONUCLEASE DIGESTION OF R17 VIRAL RNA. J Mol Biol. 1965 Jan;11:84–89. doi: 10.1016/s0022-2836(65)80173-5. [DOI] [PubMed] [Google Scholar]
  22. SMITH K. O., MELNICK J. L. A method for staining virus particles and identifying their nucleic acid type in the electron microscope. Virology. 1962 Jul;17:480–490. doi: 10.1016/0042-6822(62)90143-5. [DOI] [PubMed] [Google Scholar]
  23. TRAUTMAN R., BREESE S. S., Jr Isodensity ultracentrifugation of foot-and-mouth disease virus in caesium chloride. J Gen Microbiol. 1962 Feb;27:231–239. doi: 10.1099/00221287-27-2-231. [DOI] [PubMed] [Google Scholar]
  24. Watanabe Y., Watanabe K., Katagiri S., Hinuma Y. Virus-specific proteins produced in HeLa cells infected with poliovirus: characterization of subunit-like protein. J Biochem. 1965 Jun;57(6):733–741. doi: 10.1093/oxfordjournals.jbchem.a128139. [DOI] [PubMed] [Google Scholar]

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