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. 1968 Apr;2(4):365–375. doi: 10.1128/jvi.2.4.365-375.1968

Structure and Leukemogenic Activity of a Murine Leukemia Virus

L de Tkaczevski 1,2, E de Harven 1,2, C Friend 1,2
PMCID: PMC375622  PMID: 5742040

Abstract

Purified Friend viruses obtained from chronically infected tissue cultures were studied under the electron microscope in an effort to correlate the fine structure of the particles to their leukemogenic activity under varied experimental conditions, i.e., temperature treatments and exposure to Tween 80, amyl acetate, or ether. It was observed that an intact viral envelope was a prerequisite to leukemogenic activity as tested by intraperitoneal inoculation of newborn mice. It was also noted that the percentage of C particles was not increased after heating for 1 hr at 45 C (treatment which, however, completely inactivated the viruses). Digestion with ribonuclease indicated the presence of ribonucleic acid within the nucleoids of “enveloped A particles,” which shows that these are not immature particles. The significance of the simultaneous presence of “enveloped A” and C particles is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNHARD W., GUERIN M. Presence de particules d'aspect virusal dans les tissus tumoraux de souris atteintes de leucémie spontanée. C R Hebd Seances Acad Sci. 1958 Nov 17;247(20):1802–1805. [PubMed] [Google Scholar]
  2. Barski G., Youn J. K. Protective effect of specific immunization in Rauscher leukemia. Natl Cancer Inst Monogr. 1966 Sep;22:659–669. [PubMed] [Google Scholar]
  3. DALTON A. J., LAW L. W., MOLONEY J. B., MANAKER R. A. An electron microscopic study of a series of murine lymphoid neoplasms. J Natl Cancer Inst. 1961 Oct;27:747–791. [PubMed] [Google Scholar]
  4. DE HARVEN E. A new technique for carbon films. J Biophys Biochem Cytol. 1958 Jan 25;4(1):133–134. doi: 10.1083/jcb.4.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DE HARVEN E., FRIEND C. Electron microscope study of a cell-free induced leukemia of the mouse: a preliminary report. J Biophys Biochem Cytol. 1958 Mar 25;4(2):151–156. doi: 10.1083/jcb.4.2.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. DE HARVEN E., FRIEND C. Further electron microscope studies of a mouse leukemia induced by cell-free filtrates. J Biophys Biochem Cytol. 1960 Jul;7:747–752. doi: 10.1083/jcb.7.4.747. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DE HARVEN E. VIREMIA IN FRIEND MURINE LEUKEMIA: THE ELECTRON MICROSCOPE APPROACH TO THE PROBLEM. Pathol Biol. 1965 Feb;13:125–134. [PubMed] [Google Scholar]
  8. De Harven E., Friend C. Structure of virus particles, partially purified from the blood of leukemic mice. Virology. 1964 May;23(1):119–124. doi: 10.1016/s0042-6822(64)80019-2. [DOI] [PubMed] [Google Scholar]
  9. De-Thé G., O'Connor T. E. Structure of a murine leukemia virus after disruption with tween-ether and comparison with two myxoviruses. Virology. 1966 Apr;28(4):713–728. doi: 10.1016/0042-6822(66)90256-x. [DOI] [PubMed] [Google Scholar]
  10. Dolowy W. C. Morphology of the development of type B virus particles in a transplantable mammary tumor of ICR-Ha mice. J Natl Cancer Inst. 1965 Jun;34(6):815–825. [PubMed] [Google Scholar]
  11. FRIEND C. Cell-free transmission in adult Swiss mice of a disease having the character of a leukemia. J Exp Med. 1957 Apr 1;105(4):307–318. doi: 10.1084/jem.105.4.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Farquhar M. G., Palade G. E. Cell junctions in amphibian skin. J Cell Biol. 1965 Jul;26(1):263–291. doi: 10.1083/jcb.26.1.263. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Friend C., Patuleia M. C., De Harven E. Erythrocytic maturation in vitro of murine (Friend) virus-induced leukemic cells. Natl Cancer Inst Monogr. 1966 Sep;22:505–522. [PubMed] [Google Scholar]
  14. GRAFFI A. Chloroleukemia of mice. Ann N Y Acad Sci. 1957 Oct 21;68(2):540–558. doi: 10.1111/j.1749-6632.1957.tb56107.x. [DOI] [PubMed] [Google Scholar]
  15. GROSS L. Is leukemia caused by a transmissible virus? A working hypothesis. Blood. 1954 Jun;9(6):557–573. [PubMed] [Google Scholar]
  16. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Levy J. P., Oppenheim S., Chenaille P., Silvestre D., Tavitian A., Boiron M. Quantitative study of Rauscher virus inactivation by various physical and chemical agents. J Natl Cancer Inst. 1967 Apr;38(4):553–565. [PubMed] [Google Scholar]
  18. MOLONEY J. B. Properties of a leukemia virus. Natl Cancer Inst Monogr. 1960 Sep;4:7–37. [PubMed] [Google Scholar]
  19. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. PLUZNIK D. H., SACHS L. QUANTITATION OF A MURINE LEUKEMIA VIRUS WITH A SPLEEN COLONY ASSAY. J Natl Cancer Inst. 1964 Sep;33:535–546. [PubMed] [Google Scholar]
  21. RAUSCHER F. J. A virus-induced disease of mice characterized by erythrocytopoiesis and lymphoid leukemia. J Natl Cancer Inst. 1962 Sep;29:515–543. [PubMed] [Google Scholar]
  22. REYNOLDS E. S. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol. 1963 Apr;17:208–212. doi: 10.1083/jcb.17.1.208. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. SABATINI D. D., BENSCH K., BARRNETT R. J. Cytochemistry and electron microscopy. The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation. J Cell Biol. 1963 Apr;17:19–58. doi: 10.1083/jcb.17.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shibley G. P., Durr F. E., Schidlovsky G., Wright B. S., Schmitter R. Leukemogenic activity of ether-extracted Rauscher leukemia virus. Science. 1967 Jun 23;156(3782):1610–1613. doi: 10.1126/science.156.3782.1610. [DOI] [PubMed] [Google Scholar]
  25. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Wright B. S., Lasfargues J. C. Long-term propagation of the Rauscher murine leukemia virus in tissue culture. J Natl Cancer Inst. 1965 Aug;35(2):319–327. [PubMed] [Google Scholar]

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