Abstract
Titers of vaccinia virus consistently increased in cultures of washed phytohemagglutinin-treated, peripheral blood leukocytes of a vaccinated adult. Concomitantly, a gradual rise occurred in the numbers of infected leukocytes, as determined by the infective center assay. Increase in viral titer was accompanied by cell injury, decline in cell numbers, and decreased acid production. Leukocytes not pretreated with phytohemagglutinin appeared to form infective centers after exposure to the vaccinia agent, but they did not replicate infectious virus. For viral replication, the continuous presence of phytohemagglutinin was required.
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- Duc-Nguyen H., Henle W. Replication of mumps virus in human leukocyte cultures. J Bacteriol. 1966 Jul;92(1):258–265. doi: 10.1128/jb.92.1.258-265.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edelman R., Wheelock E. F. Vesicular stomatitis virus replication in human leukocyte cultures: enhancement by phytohemagglutinin. Science. 1966 Nov 25;154(3752):1053–1055. doi: 10.1126/science.154.3752.1053. [DOI] [PubMed] [Google Scholar]
- FURNESS G., YOUNGNER J. S. One-step growth curves for vaccinia virus in cultures of monkey kidney cells. Virology. 1959 Nov;9:386–395. doi: 10.1016/0042-6822(59)90130-8. [DOI] [PubMed] [Google Scholar]
- JAHMIAS A. J., KIBRICK S., ROSAN R. C. VIRAL LEUKOCYTE INTERRELATIONSHIPS. I. MULTIPLICATION OF A DNA VIRUS--HERPES SIMPLEX--IN HUMAN LEUKOCYTE CULTURES. J Immunol. 1964 Jul;93:69–74. [PubMed] [Google Scholar]
- MOORHEAD P. S., NOWELL P. C., MELLMAN W. J., BATTIPS D. M., HUNGERFORD D. A. Chromosome preparations of leukocytes cultured from human peripheral blood. Exp Cell Res. 1960 Sep;20:613–616. doi: 10.1016/0014-4827(60)90138-5. [DOI] [PubMed] [Google Scholar]
- NISHMI M., BERNKOPF H. The toxic effect of vaccinia virus on leucocytes in vitro. J Immunol. 1958 Dec;81(6):460–466. [PubMed] [Google Scholar]
- RABINOWITZ Y. SEPARATION OF LYMPHOCYTES, POLYMORPHONUCLEAR LEUKOCYTES AND MONOCYTES ON GLASS COLUMNS, INCLUDING TISSUE CULTURE OBSERVATIONS. Blood. 1964 Jun;23:811–828. [PubMed] [Google Scholar]
- Wheelock E. F. Interferon-like virus-inhibitor induced in human leukocytes by phytohemagglutinin. Science. 1965 Jul 16;149(3681):310–311. [PubMed] [Google Scholar]