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. 1969 Feb;3(2):164–170. doi: 10.1128/jvi.3.2.164-170.1969

Biological Activity of 5-Hydroxymethyluracil and Its Deoxynucleoside in Noninfected and Phageinfected Bacillus subtilis

Mutsuko Nishihara 1,1, Norman Friedman 1, H Vasken Aposhian 1
PMCID: PMC375747  PMID: 4975365

Abstract

14C-hydroxymethyldeoxyuridine (dHMU) is specifically incorporated into the deoxyribonucleic acid (DNA) of bacteriophage SP8. Incorporation experiments demonstrate that the initiation of phage SP8 DNA synthesis occurs between 12.5 to 15 min after infection. Incorporation into host DNA does not occur. 14C-dHMU can be used as an analytical tool for screening conditionally lethal phage mutants containing hydroxymethyluracil in their DNA to select those that are defective in DNA synthesis under restrictive conditions. The pyrimidine, 14C-hydroxymethyluracil (HMU), is not incorporated into bacterial or phage DNA. Neither HMU nor dHMU can replace thymine as a growth requirement for Bacillus subtilis 168 Ind Thy. HMU does not inhibit the utilization of thymine. Although dHMU inhibits deoxythymidine utilization, the inhibition is not competitive.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aposhian H. V., Tremblay G. Y. Deoxythymidylate 5'-nucleotidase. Purification and properties of an enzyme found after infection of Bacillus subtilis with phage SP5C. J Biol Chem. 1966 Nov 10;241(21):5095–5101. [PubMed] [Google Scholar]
  2. DUNN D. B., SMITH J. D. Effects of 5-halogenated uracils on the growth of Escherichia coli and their incorporation into deoxyribonucleic acids. Biochem J. 1957 Nov;67(3):494–506. doi: 10.1042/bj0670494. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DUNN D. B., SMITH J. D., ZAMENHOF S., GRIBOFF G. Incorporation of halogenated pyrimidines into the deoxyribonucleic acids of Bacterium coli and its bacteriophages. Nature. 1954 Aug 14;174(4424):305–307. [PubMed] [Google Scholar]
  4. FARMER J. L., ROTHMAN F. TRANSFORMABLE THYMINE-REQUIRING MUTANT OF BACILLUS SUBTILS. J Bacteriol. 1965 Jan;89:262–263. doi: 10.1128/jb.89.1.262-263.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GREEN D. M. INFECTIVITY OF DNA ISOLATED FROM BACILLUS SUBTILIS BACTERIOPHAGE, SP82. J Mol Biol. 1964 Dec;10:438–451. doi: 10.1016/s0022-2836(64)80065-6. [DOI] [PubMed] [Google Scholar]
  6. Kahan E. A genetic study of temperature-sensitive mutants of the subtilis phage SP82. Virology. 1966 Dec;30(4):650–660. doi: 10.1016/0042-6822(66)90170-x. [DOI] [PubMed] [Google Scholar]
  7. MAALOE O., HANAWALT P. C. Thymine deficiency and the normal DNA replication cycle. I. J Mol Biol. 1961 Apr;3:144–155. doi: 10.1016/s0022-2836(61)80041-7. [DOI] [PubMed] [Google Scholar]
  8. Nishihara M., Chrambach A., Aposhian H. V. The deoxycytidylate deaminase found in Bacillus subtilis infected with phage SP8. Biochemistry. 1967 Jul;6(7):1877–1886. doi: 10.1021/bi00859a001. [DOI] [PubMed] [Google Scholar]
  9. OKUBO S., STRAUSS B., STODOLSKY M. THE POSSIBLE ROLE OF RECOMBINATION IN THE INFECTION OF COMPETENT BACILLUS SUBTILIS BY BACTERIOPHAGE DEOXYRIBONUCLEIC ACID. Virology. 1964 Dec;24:552–562. doi: 10.1016/0042-6822(64)90207-7. [DOI] [PubMed] [Google Scholar]
  10. Roscoe D. H., Tucker R. G. The biosynthesis of 5-hydroxymethyldeoxyuridylic acid in bacteriophage-infected Bacillus subtilis. Virology. 1966 May;29(1):157–166. doi: 10.1016/0042-6822(66)90205-4. [DOI] [PubMed] [Google Scholar]
  11. Roscoe D. H., Tucker R. G. The biosynthesis of a pyrimidine replacing thymine in bacteriophage DNA. Biochem Biophys Res Commun. 1964 Jun 1;16(2):106–110. doi: 10.1016/0006-291x(64)90344-4. [DOI] [PubMed] [Google Scholar]
  12. Spizizen J. TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE. Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072–1078. doi: 10.1073/pnas.44.10.1072. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Trilling D. M., Aposhian H. V. A deoxyribonuclease found after infection of Bacillus subtilis with phage SP3. Proc Natl Acad Sci U S A. 1965 Aug;54(2):622–628. doi: 10.1073/pnas.54.2.622. [DOI] [PMC free article] [PubMed] [Google Scholar]

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