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. 1969 May;3(5):451–457. doi: 10.1128/jvi.3.5.451-457.1969

Replication of Poliovirus in Phytohemagglutinin-stimulated Human Lymphocytes

Frank T C Willems 1,1, Joseph L Melnick 1, William E Rawls 1
PMCID: PMC375793  PMID: 4306718

Abstract

Poliovirus replication has been studied in human lymphocytes during the course of blastogenesis under phytohemagglutinin (PHA) stimulation. Enhancement of virus replication in PHA-stimulated leukocyte cultures was due to an increase in number of virus-producing cells. Virus yield was approximately 10 plaque-forming units (PFU) per producing cell, both in stimulated and in nonstimulated cultures. Adsorption and penetration studies showed that freshly drawn lymphocytes (unlike other leukocytes) were resistant to virus infection, but they became susceptible to the virus during PHA stimulation. Also, the eclipse of the virus after penetration was enhanced during blastogenesis of the lymphocytes. Our findings suggested that the monocytes in the leukocyte cultures were infected initially. In PHA-stimulated cultures, the virus then spread to lymphocytes which became susceptible to virus infection during blastogenesis. Polymorphonuclear cells died within 24 to 48 hr after initiation of the cultures and apparently could not support poliovirus replication.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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