Abstract
Morphogenesis of Venezuelan equine encephalomyelitis virus was studied by means of electron microscopy. Virus-specific structures (factories, viroplasts) were found at early stages of infection; these structures were composed of fibrillar and cylindrical formations, aggregates of ribosomes, and viral nucleoids. The latter emerged from fibrillar and cylindrical structures. Aggregates of viral nucleoids were found in the cytoplasm and occasionally in the nuclei of virus-infected cells. Viral envelopes and mature virions were formed on the cell membranes and on the membranes of intracellular vacuoles. Anomalous forms of virions (both polygenomic and oligogenomic) were observed.
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- Grimley P. M., Berezesky I. K., Friedman R. M. Cytoplasmic structures associated with an arbovirus infection: loci of viral ribonucleic acid synthesis. J Virol. 1968 Nov;2(11):1326–1338. doi: 10.1128/jvi.2.11.1326-1338.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HENDERSON J. R., TAYLOR R. M. Propagation of certain arthropod-borne viruses in avian and primate cell cultures. J Immunol. 1960 Jun;84:590–598. [PubMed] [Google Scholar]
- KARNOVSKY M. J. Simple methods for "staining with lead" at high pH in electron microscopy. J Biophys Biochem Cytol. 1961 Dec;11:729–732. doi: 10.1083/jcb.11.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Klimenko S. M., Yershov F. I., Gofman Y. P., Nabatnikov A. P., Zhdanov V. M. Architecture of Venezuelan equine encephalomyelitis virus. Virology. 1965 Oct;27(2):125–128. doi: 10.1016/0042-6822(65)90152-2. [DOI] [PubMed] [Google Scholar]
- MORGAN C., HOWE C., ROSE H. M. Structure and development of viruses as observed in the electron microscope. V. Western equine encephalomyelitis virus. J Exp Med. 1961 Jan 1;113:219–234. doi: 10.1084/jem.113.1.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MUSSGAY M. STUDIES ON THE STRUCTURE OF A HEMAGGLUTINATING COMPONENT OF A GROUP A ARBO VIRUS (SINDBIS). Virology. 1964 Aug;23:573–581. doi: 10.1016/0042-6822(64)90241-7. [DOI] [PubMed] [Google Scholar]
- Stevens T. M., Schlesinger R. W. Studies on the nature of dengue viruses. I. Correlation of particle density, infectivity, and RNA content of type 2 virus. Virology. 1965 Sep;27(1):103–112. doi: 10.1016/0042-6822(65)90147-9. [DOI] [PubMed] [Google Scholar]
- Yershov F. I., Zhdanov V. M. Relationship between group A arbovirus reproduction and cytochemical changes in infected cells. Acta Virol. 1968 Mar;12(2):136–143. [PubMed] [Google Scholar]
- Zhdanov V. M., Yershov F. I., Uryvayev L. V. Ribonucleic acids synthesized in Venezuelan equine encephalomyelitis virus-infected cells. Virology. 1969 Jun;38(2):355–358. doi: 10.1016/0042-6822(69)90380-8. [DOI] [PubMed] [Google Scholar]