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. 1970 Feb;5(2):145–152. doi: 10.1128/jvi.5.2.145-152.1970

Purification, Characterization, and Attempts at Isotopic Labeling of Mouse Interferon

K Paucker 1, B J Berman 1, R R Golgher 1,1, D Stanček 1,2
PMCID: PMC375980  PMID: 4317345

Abstract

Under optimal conditions which minimized the accumulation of extraneous proteins, interferon preparations were obtained in L cells containing from 2 × 104 to 5 × 104 units/mg of protein. The radiolabeled proteins were liberated simultaneously with interferon from cultures exposed to tritiated amino acids after viral stimulation and from corresponding controls, and were subsequently purified with the following results. Chromatography of interferon on carboxymethyl-Sephadex C-25 eliminated selectively unlabeled or poorly labeled proteins, resulting in a greater than sixfold increase in counts per minute per milligram of protein. Similarly purified control material harbored at least 12 times less tritium per milligram of protein than interferon, and the label was more diversely distributed among proteins of different molecular weights. On electrophoresis of interferon in polyacrylamide gels, labeled proteins were reduced further by a factor of at least 10 without loss in titer. Final purification was estimated at greater than 280-fold, representing a calculated specific activity of at least 1.4 × 107 units of interferon per milligram of protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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