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. 1970 Mar;5(3):388–397. doi: 10.1128/jvi.5.3.388-397.1970

Cell-Free Transmission and In Vivo Replication of Marek's Disease Virus 1

K Nazerian 1, R L Witter 1
PMCID: PMC376016  PMID: 4191324

Abstract

Marek's disease virus recovered from the feather follicle of infected chickens was found to be infectious for chickens in cell-free preparations. The virus replicated in epithelial cells of the germinative layer of the feather follicle epidermis, producing both intranuclear and round or diffuse cytoplasmic inclusion bodies in the infected cells. It was found at this site 2 weeks postinoculation and prior to the development of tumor or other gross lesions. In the nucleus, many naked and a few enveloped herpesvirions were found, whereas the cytoplasm contained predominantly enveloped herpesvirions, which were usually within the cytoplasmic inclusion bodies. Approximately 80% of the extracellular virions were enveloped. Studies with both virulent and avirulent strains of the virus revealed a relationship between virulence, contagiousness, and replication of the virus in the feather follicle.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ahmed M., Schidlovsky G. Electron microscopic localization of herpesvirus-type particles in Marek's disease. J Virol. 1968 Dec;2(12):1443–1457. doi: 10.1128/jvi.2.12.1443-1457.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Calnek B. W., Hitchner S. B. Localization of viral antigen in chickens infected with Marek's disease herpesvirus. J Natl Cancer Inst. 1969 Oct;43(4):935–949. [PubMed] [Google Scholar]
  3. Churchill A. E., Biggs P. M. Agent of Marek's disease in tissue culture. Nature. 1967 Jul 29;215(5100):528–530. doi: 10.1038/215528a0. [DOI] [PubMed] [Google Scholar]
  4. Churchill A. E., Biggs P. M. Herpes-type virus isolated in cell culture from tumors of chickens with Marek's disease. II. Studies in vivo. J Natl Cancer Inst. 1968 Oct;41(4):951–956. [PubMed] [Google Scholar]
  5. Churchill A. E. Herpes-type virus isolated in cell culture from tumors of chickens with Marek's disease. I. Studies in cell culture. J Natl Cancer Inst. 1968 Oct;41(4):939–950. [PubMed] [Google Scholar]
  6. Churchill A. E., Payne L. N., Chubb R. C. Immunization against Marek's disease using a live attenuated virus. Nature. 1969 Feb 22;221(5182):744–747. doi: 10.1038/221744a0. [DOI] [PubMed] [Google Scholar]
  7. HUXLEY H. E., ZUBAY G. Preferential staining of nucleic acid-containing structures for electron microscopy. J Biophys Biochem Cytol. 1961 Nov;11:273–296. doi: 10.1083/jcb.11.2.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. KARNOVSKY M. J. Simple methods for "staining with lead" at high pH in electron microscopy. J Biophys Biochem Cytol. 1961 Dec;11:729–732. doi: 10.1083/jcb.11.3.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LUFT J. H. Improvements in epoxy resin embedding methods. J Biophys Biochem Cytol. 1961 Feb;9:409–414. doi: 10.1083/jcb.9.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nazerian K., Burmester B. R. Electron microscopy of a herpes virus associated with the agent of Marek's disease in cell culture. Cancer Res. 1968 Dec;28(12):2454–2462. [PubMed] [Google Scholar]
  11. Nazerian K., Solomon J. J., Witter R. L., Burmester B. R. Studies on the etiology of Marek's disease. II. Finding of a herpesvirus in cell culture. Proc Soc Exp Biol Med. 1968 Jan;127(1):177–182. doi: 10.3181/00379727-127-32650. [DOI] [PubMed] [Google Scholar]
  12. Schidlovsky G., Ahmed M., Jensen K. E. Herpesvirus in Marek's disease tumors. Science. 1969 May 23;164(3882):959–961. doi: 10.1126/science.164.3882.959. [DOI] [PubMed] [Google Scholar]
  13. Solomon J. J., Witter R. L., Nazerian K., Burmester B. R. Studies on the etiology of Marek's disease. I. Propagation of the agent in cell culture. Proc Soc Exp Biol Med. 1968 Jan;127(1):173–177. doi: 10.3181/00379727-127-32649. [DOI] [PubMed] [Google Scholar]
  14. Witter R. L., Burgoyne G. H., Solomon J. J. Evidence for a herpesvirus as an etiologic agent of marek's disease. Avian Dis. 1969 Feb;13(1):171–184. [PubMed] [Google Scholar]
  15. Witter R. L., Burgoyne G. H., Solomon J. J. Preliminary studies on cell cultures infected with Marek's disease agent. Avian Dis. 1968 Feb;12(1):169–185. [PubMed] [Google Scholar]
  16. Witter R. L., Solomon J. J., Burgoyne G. H. Cell culture techniques for primary isolation of Marek's disease-associated herpesvirus. Avian Dis. 1969 Feb;13(1):101–118. [PubMed] [Google Scholar]

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