Abstract
A virus isolated from a flying fox (Pteropus poliocephalus) has a morphology similar to that of reoviruses. However, unlike the reoviruses, this virus has a rapid cytopathic effect, causing cell fusion, vacuolization of the cytoplasm, and an unusual nuclear degeneration. Immunofluorescence indicates that viral antigen is distributed through both nucleus and cytoplasm. Viral maturation appears to take place in discrete cytoplasmic factories, and there is no apparent involvement of spindle tubules.
Full text
PDF






Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BAZELEY P. L., THAYER J. R. The growth of poliomyelitis virus in tissue culture. I. The use of a stationary bottle technique. Aust J Exp Biol Med Sci. 1954 Feb;32(1):23–27. doi: 10.1038/icb.1954.2. [DOI] [PubMed] [Google Scholar]
- BILS R. F., HALL C. E. Electron microscopy of wound-tumor virus. Virology. 1962 May;17:123–130. doi: 10.1016/0042-6822(62)90089-2. [DOI] [PubMed] [Google Scholar]
- DALES S. ASSOCIATION BETWEEN THE SPINDLE APPARATUS AND REOVIRUS. Proc Natl Acad Sci U S A. 1963 Aug;50:268–275. doi: 10.1073/pnas.50.2.268. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DALES S., GOMATOS P. J., HSU K. C. THE UPTAKE AND DEVELOPMENT OF REOVIRUS IN STRAIN L CELLS FOLLOWED WITH LABELED VIRAL RIBONUCLEIC ACID AND FERRITIN-ANTIBODY CONJUGATES. Virology. 1965 Feb;25:193–211. doi: 10.1016/0042-6822(65)90199-6. [DOI] [PubMed] [Google Scholar]
- DROUHET V. Sur l'effet cytopathogène du virus ECHO 10. Ann Inst Pasteur (Paris) 1958 Dec;95(6):781–784. [PubMed] [Google Scholar]
- DROUHET V. [Cellular lesions provoked by reoviruses (ECHO virus 10). Fluorescent antibodies and cytochemical study]. Ann Inst Pasteur (Paris) 1960 Apr;98:618–621. [PubMed] [Google Scholar]
- DULBECCO R., VOGT M. Plaque formation and isolation of pure lines with poliomyelitis viruses. J Exp Med. 1954 Feb;99(2):167–182. doi: 10.1084/jem.99.2.167. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GOMATOS P. J., TAMM I., DALES S., FRANKLIN R. M. Reovirus type 3: physical characteristics and interaction with L cells. Virology. 1962 Jul;17:441–454. doi: 10.1016/0042-6822(62)90139-3. [DOI] [PubMed] [Google Scholar]
- HAWKES R. A. ENHANCEMENT OF THE INFECTIVITY OF ARBOVIRUSES BY SPECIFIC ANTISERA PRODUCED IN DOMESTIC FOWLS. Aust J Exp Biol Med Sci. 1964 Aug;42:465–482. doi: 10.1038/icb.1964.44. [DOI] [PubMed] [Google Scholar]
- Petek M., Felluga B., Borghi G., Baroni A. The Crawley agent: an avian reovirus. Arch Gesamte Virusforsch. 1967;21(3):413–424. doi: 10.1007/BF01241740. [DOI] [PubMed] [Google Scholar]
- RHIM J. S., JORDAN L. E., MAYOR H. D. Cytochemical, fluorescent-antibody and electron microscopic studies on the growth of reovirus (ECHO 10) in tissue culture. Virology. 1962 Jun;17:342–355. doi: 10.1016/0042-6822(62)90125-3. [DOI] [PubMed] [Google Scholar]
- RHIM J. S., SMITH K. O., MELNICK J. L. Complete and coreless forms of reovirus (ECHO 10). Ratio of number of virus particles to infective units in the one-step growth cycle. Virology. 1961 Dec;15:428–435. doi: 10.1016/0042-6822(61)90110-6. [DOI] [PubMed] [Google Scholar]
- SALK J. E., YOUNGNER J. S., WARD E. N. Use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue-culture system. Am J Hyg. 1954 Sep;60(2):214–230. doi: 10.1093/oxfordjournals.aje.a119714. [DOI] [PubMed] [Google Scholar]
- SPENDLOVE R. S., LENNETTE E. H., JOHN A. C. THE ROLE OF THE MITOTIC APPARATUS IN THE INTRACELLULAR LOCATION OF REOVIRUS ANTIGEN. J Immunol. 1963 Apr;90:554–560. [PubMed] [Google Scholar]
- Schnagl R. D., Holmes I. H., Doherty R. L. An electron microscopic study of Eubenangee, an Australian arbovirus. Virology. 1969 Jun;38(2):347–349. doi: 10.1016/0042-6822(69)90377-8. [DOI] [PubMed] [Google Scholar]
- Shimizu Y., Furuuchi S., Hayashi S., Kumagai T., Sasahara J. Porcine kidney cell line persistently contaminated with avirulent swine fever virus. J Gen Virol. 1969 Jun;4(4):625–628. doi: 10.1099/0022-1317-4-4-625. [DOI] [PubMed] [Google Scholar]
- TOURNIER P., PLISSIER M. [The intracellular development of the reovirus studied with the electron microscope]. Presse Med. 1960 Apr 9;68:683–688. [PubMed] [Google Scholar]
- Tikasingh E. S., Spence L., Downs W. G. The use of adjuvant and sarcoma 180 cells in the production of mouse hyperimmune ascitic fluids to arboviruses. Am J Trop Med Hyg. 1966 Mar;15(2):219–226. doi: 10.4269/ajtmh.1966.15.219. [DOI] [PubMed] [Google Scholar]
- VASQUEZ C., TOURNIER P. NEW INTERPRETATION OF THE REOVIRUS STRUCTURE. Virology. 1964 Sep;24:128–130. doi: 10.1016/0042-6822(64)90162-x. [DOI] [PubMed] [Google Scholar]
- VASQUEZ C., TOURNIER P. The morphology of reovirus. Virology. 1962 Aug;17:503–510. doi: 10.1016/0042-6822(62)90149-6. [DOI] [PubMed] [Google Scholar]
- Westaway E. G. Assessment and application of a cell line from pig kidney for plaque assay and neutralization tests with twelve group B arboviruses. Am J Epidemiol. 1966 Nov;84(3):439–456. doi: 10.1093/oxfordjournals.aje.a120657. [DOI] [PubMed] [Google Scholar]