Abstract
A general procedure is described for isolation of T-even phage-tolerant mutants of Escherichia coli. Two such mutants of E. coli B have been examined in some detail. These mutants adsorb T-even phages but are unable to release viable progeny. Under certain conditions, viability of the cells is completely unaffected by phage infection in one mutant, and there is but a slight decrease in colony-forming ability in the other. Phage deoxyribonucleic acid (DNA) is injected into these cells, as shown by the formation of phage-specific enzymes, but it is not degraded to acid-soluble material. Some phage DNA replication occurs in both strains. The mutants are both more resistant to ultraviolet light than is the parent strain.
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Selected References
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- Calendar R., Lindqvist B., Sironi G., Clark A. J. Characterization of REP- mutants and their interaction with P2 phage. Virology. 1970 Jan;40(1):72–83. doi: 10.1016/0042-6822(70)90380-6. [DOI] [PubMed] [Google Scholar]
- Denhardt D. T., Dressler D. H., Hathaway A. THE ABORTIVE REPLICATION OF PhiX174 DNA IN A RECOMBINATION-DEFICIENT MUTANT OF Escherichia coli. Proc Natl Acad Sci U S A. 1967 Mar;57(3):813–820. doi: 10.1073/pnas.57.3.813. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FRASER D., JERREL E. A. The amino acid composition of T3 bacteriophage. J Biol Chem. 1953 Nov;205(1):291–295. [PubMed] [Google Scholar]
- Frankel F. R. Evidence for long DNA strands in the replicating pool after T4 infection. Proc Natl Acad Sci U S A. 1968 Jan;59(1):131–138. doi: 10.1073/pnas.59.1.131. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KOZINSKI A. W. Fragmentary transfer of P32-labeled parental DNA to progeny phage. Virology. 1961 Jan;13:124–134. doi: 10.1016/0042-6822(61)90039-3. [DOI] [PubMed] [Google Scholar]
- Kozinski A. W., Felgenhauer Z. Z. Molecular recombination in T4 bacteriophage deoxyribonucleic acid. II. Single-strand breaks and exposure of uncomplemented areas as a prerequisite for recombination. J Virol. 1967 Dec;1(6):1193–1202. doi: 10.1128/jvi.1.6.1193-1202.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matthews C. K. Deoxyribonucleic acid metabolism and virus-induced enzyme synthesis in a thymine-requiring bacterium infected by a thymine-requiring bacteriophage. Biochemistry. 1966 Jun;5(6):2092–2100. doi: 10.1021/bi00870a042. [DOI] [PubMed] [Google Scholar]
- Murray R. E., Mathews C. K. Addition of nucleotides to parental DNA early in infection by bacteriophage T4. J Mol Biol. 1969 Sep 14;44(2):233–248. doi: 10.1016/0022-2836(69)90172-7. [DOI] [PubMed] [Google Scholar]
- Naha P. M. Bacterial control of lambda replication. I. Initial characterization of a rep mutation of Escherichia coli K12. Virology. 1968 Nov;36(3):434–441. doi: 10.1016/0042-6822(68)90168-2. [DOI] [PubMed] [Google Scholar]
- Smith H. S., Miovic M., Pizer L. I. Correlation between degradation of bacteriophage T2 deoxyribonucleic acid and the resistance of Escherichia coli to infection. J Virol. 1969 Aug;4(2):195–196. doi: 10.1128/jvi.4.2.195-196.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WEIDEL W. Bacterial viruses; with particular reference to adsorption/penetration. Annu Rev Microbiol. 1958;12:27–48. doi: 10.1146/annurev.mi.12.100158.000331. [DOI] [PubMed] [Google Scholar]