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. 1970 Sep;6(3):340–343. doi: 10.1128/jvi.6.3.340-343.1970

Genetic Mapping of a Defective Bacteriophage on the Chromosome of Bacillus subtilis 168

Anthony J Garro 1,1, Hyam Leffert 1, Julius Marmur 1
PMCID: PMC376128  PMID: 4991424

Abstract

A genetic marker responsible for the killing activity of PBSX, a defective phage carried by Bacillus subtilis 168, has been located on the bacterial chromosome. Two mutant strains of B. subtilis 168, which produced tailless phage particles upon mitomycin C induction, were shown to carry lesions, designated xtl-1 and xtl-2, which were linked by transformation and PBS1-mediated transduction to metC. The link-age relationship between xtl and adjacent auxotrophic markers was determined by three-factor PBS1 transduction, the suggested order of markers being argO 1 metA metC xtl.

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Selected References

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