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. 1970 Oct;6(4):559–562. doi: 10.1128/jvi.6.4.559-562.1970

Incorporation of Deoxyribonucleic Acid Precursors by T4 Deoxyribonucleic Acid-Protein Complexes Retained on Glass Fiber Filters

Robert C Miller Jr 1,1, Andrzej W Kozinski 1
PMCID: PMC376155  PMID: 5497903

Abstract

Bacteriophage T4 deoxyribonucleic acid (DNA)-protein complexes were retained preferentially on glass fiber filters. DNA polymerase activity in the complex was detected through the incorporation of 3H-labeled DNA precursors. The primer-product DNA hybridized with both phage and Escherichia coli DNA. Density labeling experiments showed that about 30% of incorporated 3H-deoxyadenosine triphosphate was found in DNA which hybridized with phage DNA; this DNA was found to be covalently attached to the primer DNA.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. APOSHIAN H. V., KORNBERG A. Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme. J Biol Chem. 1962 Feb;237:519–525. [PubMed] [Google Scholar]
  2. De Waard A., Paul A. V., Lehman I. R. The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1241–1248. doi: 10.1073/pnas.54.4.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Denhardt D. T. A membrane-filter technique for the detection of complementary DNA. Biochem Biophys Res Commun. 1966 Jun 13;23(5):641–646. doi: 10.1016/0006-291x(66)90447-5. [DOI] [PubMed] [Google Scholar]
  4. Guha A., Szybalski W. Fractionation of the complementary strands of coliphage T4 DNA based on the asymmetric distribution of the poly U and poly U,G binding sites. Virology. 1968 Apr;34(4):608–616. doi: 10.1016/0042-6822(68)90082-2. [DOI] [PubMed] [Google Scholar]
  5. KOZINSKI A. W., SZYBALSKI W. Dispersive transfer of the parental DNA molecule to the progeny of phage phiX-174. Virology. 1959 Oct;9:260–274. doi: 10.1016/0042-6822(59)90119-9. [DOI] [PubMed] [Google Scholar]
  6. Kozinski A. W., Kozinski P. B. Early intracellular events in the replication T4 phage DNA. II. Partially replicated DNA. Proc Natl Acad Sci U S A. 1965 Aug;54(2):634–640. doi: 10.1073/pnas.54.2.634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kozinski A. W., Lin T. H. Early intracellular events in the replication of T4 phage DNA. I. Complex formation of replicative DNA. Proc Natl Acad Sci U S A. 1965 Jul;54(1):273–278. doi: 10.1073/pnas.54.1.273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kozinski A. W. Unbiased participation of T4 phage DNA strands in replication. Biochem Biophys Res Commun. 1969 Apr 29;35(2):294–299. doi: 10.1016/0006-291x(69)90281-2. [DOI] [PubMed] [Google Scholar]
  9. Miller R. C., Jr, Buckley P. Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. VI. Newly synthesized proteins in the T4 protein-deoxyribonucleic acid complex. J Virol. 1970 Apr;5(4):502–506. doi: 10.1128/jvi.5.4.502-506.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Miller R. C., Jr, Kozinski A. W. Early intracellular events in the replication of bacteriophage T4 deoxyribonucleic acid. V. Further studies on the T4 protein-deoxyribonucleic acid complex. J Virol. 1970 Apr;5(4):490–501. doi: 10.1128/jvi.5.4.490-501.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]

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