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. 1971 Oct;22(4):604–607. doi: 10.1128/am.22.4.604-607.1971

Comparison of Two Commercially Available Media for Detection of Bacteremia

John A Washington II 1
PMCID: PMC376371  PMID: 4943274

Abstract

An analysis of 3,795 positive blood cultures obtained from 1,718 patients in a 2.5-year evaluation of Tryptic Soy Broth (TSB) and Thiol Broth is reported. Isolation rates of Actinobacillus and Pseudomonas were significantly greater in TSB, whereas isolation rates of Streptococcus and Corynebacterium (aerobic and anaerobic) were significantly greater in Thiol. Otherwise, the two media were similar. Disregarding contaminants, anaerobic bacteria represented 11% of positive cultures and 20% of patients with bacteremia. Eleven per cent of the patients had polymicrobial bacteremia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. COCHRAN W. G. The comparison of percentages in matched samples. Biometrika. 1950 Dec;37(3-4):256–266. [PubMed] [Google Scholar]
  2. Ellner P. D., Stoessel C. J. The role of temperature and anticoagulant on the in vitro survival of bacterial in blood. J Infect Dis. 1966 Apr;116(2):238–242. doi: 10.1093/infdis/116.2.238. [DOI] [PubMed] [Google Scholar]
  3. Ellner P. D. System for inoculation of blood in the laboratory. Appl Microbiol. 1968 Dec;16(12):1892–1894. doi: 10.1128/am.16.12.1892-1894.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Finegold S. M., White M. L., Ziment I., Winn W. R. Rapid diagnosis of bacteremia. Appl Microbiol. 1969 Sep;18(3):458–463. doi: 10.1128/am.18.3.458-463.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Finegold S. M., Ziment I., White M. L., Winn W. R., Carter W. T. Evaluation of polyanethol sulfonate (liquoid) in blood cultures. Antimicrob Agents Chemother (Bethesda) 1967;7:692–696. [PubMed] [Google Scholar]
  6. Hermans P. E., Washington J. A., 2nd Polymicrobial bacteremia. Ann Intern Med. 1970 Sep;73(3):387–392. doi: 10.7326/0003-4819-73-3-387. [DOI] [PubMed] [Google Scholar]
  7. Morello J. A., Ellner P. D. New medium for blood cultures. Appl Microbiol. 1969 Jan;17(1):68–70. doi: 10.1128/am.17.1.68-70.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Rosner R. Comparison of a blood culture system containing liquoid and sucrose with systems containing either reagent alone. Appl Microbiol. 1970 Feb;19(2):281–282. doi: 10.1128/am.19.2.281-282.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rosner R. Effect of various anticoagulants and no anticoagulant on ability to isolate bacteria directly from parallel clinical blood specimens. Am J Clin Pathol. 1968 Feb;49(2):216–219. doi: 10.1093/ajcp/49.2.216. [DOI] [PubMed] [Google Scholar]
  10. SCOTT E. G. A practical blood culture procedure. Am J Clin Pathol. 1951 Mar;21(3):290–294. doi: 10.1093/ajcp/21.3_ts.290. [DOI] [PubMed] [Google Scholar]
  11. SWAN A. The use of a bile-aesculin medium and of Maxted's technique of Lancefield grouping in the identification of enterococci (group D streptococci). J Clin Pathol. 1954 May;7(2):160–163. doi: 10.1136/jcp.7.2.160. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Washington J. A., Martin W. J., Spiekerman R. E. Brain abscess with Corynebacterium hemolyticum: report of a case. Am J Clin Pathol. 1971 Aug;56(2):212–215. doi: 10.1093/ajcp/56.2.212. [DOI] [PubMed] [Google Scholar]
  13. Werner A. S., Cobbs C. G., Kaye D., Hook E. W. Studies on the bacteremia of bacterial endocarditis. JAMA. 1967 Oct 16;202(3):199–203. [PubMed] [Google Scholar]

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