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. 1971 Nov;22(5):837–841. doi: 10.1128/am.22.5.837-841.1971

Staphylococcal Enterotoxin B: Solid-Phase Radioimmunoassay

Howard M Johnson 1, Joann A Bukovic 1, Peter E Kauffman 1, J T Peeler 1
PMCID: PMC376429  PMID: 5002312

Abstract

An immunoassay employing 125I-labeled enterotoxin B and polystyrene tubes coated with specific antibody was used for assaying purified and crude enterotoxin. Antibody was adsorbed to untreated polystyrene tubes. Unlabeled enterotoxin competed with 125I-labeled enterotoxin for antibody-combining sites. The uptake of 125I-labeled toxin reflected the concentration of unlabeled toxin present. The test is sensitive to 1 to 5 ng of purified and crude enterotoxin B per ml, and cross-reactions with heterologous enterotoxins did not interfere with the specificity. This test possesses the combination of sensitivity and objectivity absent in current methods for assaying enterotoxin and provides a model for investigating other enterotoxin serotypes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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