Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1971 Dec;22(6):1126–1134. doi: 10.1128/am.22.6.1126-1134.1971

Comparison of Conventional Methods, the R/B System, and Modified R/B System as Guides to the Major Divisions of Enterobacteriaceae

Henry D Isenberg 1, Barbara G Painter 1
PMCID: PMC376497  PMID: 4944807

Abstract

Primary grouping of more than 2,000 members of Enterobacteriaceae, freshly isolated from clinical specimens, was performed on the basis of eight conventional laboratory tests used routinely in this laboratory and by the R/B system. Results indicate that both systems perform well in this first step in the identification of various medically significant species of the family. Difficulties encountered with certain reactions of the R/B system were corrected with a physical modification of the tubes. An additional 100 representatives of Enterobacteriaceae were compared with conventional methods and the modified R/B system. Results indicate improvement in observing and interpreting findings with the R/B system.

Full text

PDF
1129

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ewing W. H., Wilfert J. N., Kunz L. J., Dumoff M., Isenberg H. D. How far to go with Enterobacteriaceae? J Infect Dis. 1969 Feb;119(2):197–213. doi: 10.1093/infdis/119.2.197. [DOI] [PubMed] [Google Scholar]
  2. ISENBERG H. D., SUNDHEIM L. H. Indole reactions in bacteria. J Bacteriol. 1958 Jun;75(6):682–690. doi: 10.1128/jb.75.6.682-690.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Isenberg H. D., Berkman J. I. Prevalence of extrachromosomal drug resistance. The role of drug-resistant and drug-selected bacteria in nosocomial disease. Ann N Y Acad Sci. 1971 Jun 11;182:52–58. doi: 10.1111/j.1749-6632.1971.tb30642.x. [DOI] [PubMed] [Google Scholar]
  4. Martin W. J., Birk R. J., Yu P. K., Washington J. A., 2nd Identification of members of the family Enterobacteriaceae by the R-B system. Appl Microbiol. 1970 Dec;20(6):880–883. doi: 10.1128/am.20.6.880-883.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. O'Donnell E. D., Kaufman F. J., Longo E. D., Ellner P. D. Evaluation of the R-B system for the identification of enterobacteriacease. Am J Clin Pathol. 1970 Feb;53(2):145–148. doi: 10.1093/ajcp/53.2.145. [DOI] [PubMed] [Google Scholar]
  6. Smith P. B., Rhoden D. L., Tomfohrde K. M., Dunn C. R., Balows A., Hermann G. J. R-B enteric differential system for identification of Enterobacteriaceae. Appl Microbiol. 1971 Jun;21(6):1036–1039. doi: 10.1128/am.21.6.1036-1039.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES