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. 1972 Sep;24(3):299–306. doi: 10.1128/am.24.3.299-306.1972

Isolation and Characterization of an Isolate (HN) of Marek's Disease Virus with Low Pathogenicity

B R Cho 1,2, S G Kenzy 1,2
PMCID: PMC376514  PMID: 4627960

Abstract

A cytopathic agent was isolated and characterized as an isolate of Marek's disease herpesvirus (MDHV) with low pathogenicity, and referred to as the HN isolate. This isolate of MDHV did not cause clinical Marek's disease (MD) or death in a highly susceptible line of chickens within 5 weeks after exposure. Gross lesions of limited extent were noted in a few of the inoculated birds. Microscopic nerve lesions in the inoculated and contact-infected birds were invariably minimal, closely resembling C-type MD lesions.

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Selected References

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  1. Anderson D. P., Eidson C. S., Richey D. J. Age susceptibility of chickens to Marek's disease. Am J Vet Res. 1971 Jun;32(6):935–938. [PubMed] [Google Scholar]
  2. Biggs P. M., Purchase H. G., Bee B. R., Dalton P. J. Preliminary report on acute Marek's disease (fowl paralysis) in Great Britain. Vet Rec. 1965 Nov 6;77(45):1339–1340. doi: 10.1136/vr.77.45.1339. [DOI] [PubMed] [Google Scholar]
  3. Biggs P. M., Thorpe R. J., Payne L. N. Studies on genetic resistance to Marek's disease in the domestic chicken. Br Poult Sci. 1968 Jan;9(1):37–52. doi: 10.1080/00071666808415692. [DOI] [PubMed] [Google Scholar]
  4. Cho B. R. Experimental dual infections of chickens with infectious bursal and Marek's disease agents. I. Preliminary observation on the effect of infectious bursal agent on Marek's disease. Avian Dis. 1970 Nov;14(4):665–675. [PubMed] [Google Scholar]
  5. Cho B. R., Kenzy S. G., Kim U. H. Atypical cells in the peripheral blood of chickens exposed to Marek's disease agent. Can J Comp Med. 1968 Oct;32(4):562–567. [PMC free article] [PubMed] [Google Scholar]
  6. Cho B. R., Kenzy S. G., Mathey W. J. Histologic and microbiologic studies of chickens with transient paralysis. Avian Dis. 1970 Nov;14(4):587–598. [PubMed] [Google Scholar]
  7. Churchill A. E. Herpes-type virus isolated in cell culture from tumors of chickens with Marek's disease. I. Studies in cell culture. J Natl Cancer Inst. 1968 Oct;41(4):939–950. [PubMed] [Google Scholar]
  8. Eidson C. S., Schmittle S. C. Studies on acute Marek's disease. I. Characteristics of isolate GA in chickens. Avian Dis. 1968 Aug;12(3):467–476. [PubMed] [Google Scholar]
  9. Hutt F. B., Cole R. K. Genetic Control of Lymphomatosis in the Fowl. Science. 1947 Oct 24;106(2756):379–384. doi: 10.1126/science.106.2756.379. [DOI] [PubMed] [Google Scholar]
  10. Okazaki W., Purchase H. G., Noll L. Effect of different conditions on precipitation in agar between Marek's disease antigen and antibody. Avian Dis. 1970 Aug;14(3):532–537. [PubMed] [Google Scholar]
  11. Purchase H. G., Biggs P. M. Characterization of five isolates of Marek's disease. Res Vet Sci. 1967 Oct;8(4):440–449. [PubMed] [Google Scholar]
  12. Purchase H. G., Burgoyne G. H. Immunofluorescence in the study of Marek's disease: detection of antibody. Am J Vet Res. 1970 Jan;31(1):117–123. [PubMed] [Google Scholar]
  13. Purchase H. G. Immunofluorescence in the study of Marek's disease. I. Detection of antigen in cell culture and an antigenic comparison of eight isolates. J Virol. 1969 Jun;3(6):557–565. doi: 10.1128/jvi.3.6.557-565.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rispens B. H., Van Vloten J., Maas H. J. Some virological and serological observations on Marek's disease: a preliminary report. Br Vet J. 1969 Sep;125(9):445–453. doi: 10.1016/s0007-1935(17)48760-4. [DOI] [PubMed] [Google Scholar]
  15. Sevoian M., Chamberlain D. M. Avian lymphomatosis. 3. Incidence and manifestations in experimentally infected chickens of various ages. Avian Dis. 1963 Feb;7(1):97–102. [PubMed] [Google Scholar]
  16. Sharma J. M., Davis W. C., Kenzy S. G. Etiologic relationship of skin tumors (skin leukosis) of chickens to Marek's disease. J Natl Cancer Inst. 1970 Apr;44(4):901–911. [PubMed] [Google Scholar]
  17. Witter R. L., Solomon J. J., Burgoyne G. H. Cell culture techniques for primary isolation of Marek's disease-associated herpesvirus. Avian Dis. 1969 Feb;13(1):101–118. [PubMed] [Google Scholar]

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