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. 1970 Jul;20(1):78–84. doi: 10.1128/am.20.1.78-84.1970

Characterization of Clostridia by Gas Chromatography: Differentiation of Species by Trimethylsilyl Derivatives of Whole-Cell Hydrolysates

David C Farshy 1, C Wayne Moss 1
PMCID: PMC376871  PMID: 4318575

Abstract

Trimethylsilyl (TMS) derivatives prepared from whole-cell hydrolysates of 36 strains, representing 10 species of Clostridium were examined by gas-liquid chromatography (GLC). The TMS profile of each species contained a group of peaks which characterized the species. Variation among strains within a species was much lower than variation between species. Some of the closely related clostridia could be differentiated by comparing their TMS profiles. Strains of Clostridium botulinum were distinguished from C. sporogenes on the basis of the ratio of two GLC peaks which corresponded to arabinose and glucose. A peak with a retention time identical to that of mannose was present in all C. bifermentans strains but was absent in those of C. sordellii.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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