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. 1971 Apr;21(4):723–725. doi: 10.1128/am.21.4.723-725.1971

Semi-Micro, Dye-Binding Assay for Rabbit Interferon

John A Armstrong 1
PMCID: PMC377263  PMID: 4325022

Abstract

The semi-micro method for interferon assay was based on quantitation of inhibition of cytopathic effects in 6-mm wells. Cultures were exposed to interferon dilutions, challenged with vesicular stomatitis virus, and stained with methylrosanaline chloride. The bound dye was then eluted and measured colorimetrically. The method was more sensitive than the plaque-inhibition method and was extremely economical. Culture age was the most important factor affecting reproductibility.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. LINDENMANN J., GIFFORD G. E. Studies on vaccinia virus plaque formation and its inhibition by interferon. III. A simplified plaque inhibition assay of interferon. Virology. 1963 Mar;19:302–309. doi: 10.1016/0042-6822(63)90068-0. [DOI] [PubMed] [Google Scholar]
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