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. 1971 Jun;21(6):993–998. doi: 10.1128/am.21.6.993-998.1971

Effects of Citrate on the Composition and Metabolism of Lactobacillus casei

A L Branen 1,1, T W Keenan 1
PMCID: PMC377330  PMID: 4327613

Abstract

Lactobacillus casei ATCC 393 converted small amounts of citrate to diacetyl, other volatile compounds, and lipids. Citrate was accumulated passively by the organism. The presence of citrate in the growth medium decreased the uptake of acetate and its conversion to cellular lipids. Cells grown in citrate media contained more protein per cell than did controls. This increased protein content was reflected mainly in the soluble fraction when cells were subjected to sonic lysis. Soluble fractions from cells cultured in the presence of citrate contained more total protein as well as more individual proteins than these fractions from control cells. The presence of citrate caused extensive flocculation and increased the susceptibility of cells to lysis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BRAGDON J. H. Colorimetric determination of blood lipides. J Biol Chem. 1951 Jun;190(2):513–517. [PubMed] [Google Scholar]
  2. Branen A. L., Keenan T. W. Diacetyl reductase of Lactobacillus casei. Can J Microbiol. 1970 Oct;16(10):947–951. doi: 10.1139/m70-162. [DOI] [PubMed] [Google Scholar]
  3. Branen A. L., Keenan T. W. Growth stimulation of Lactobacillus casei by sodium citrate. J Dairy Sci. 1970 May;53(5):593–597. doi: 10.3168/jds.S0022-0302(70)86259-2. [DOI] [PubMed] [Google Scholar]
  4. CERIOTTI G. A microchemical determination of desoxyribonucleic acid. J Biol Chem. 1952 Sep;198(1):297–303. [PubMed] [Google Scholar]
  5. CHRISTENSEN M. D., PEDERSON C. S. Factors affecting diacetyl production by lactic acid bacteria. Appl Microbiol. 1958 Sep;6(5):319–322. doi: 10.1128/am.6.5.319-322.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Campbell J. J., Gunsalus I. C. Citric Acid Fermentation by Streptococci and Lactobacilli. J Bacteriol. 1944 Jul;48(1):71–76. doi: 10.1128/jb.48.1.71-76.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. DAVIS B. J. DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS. Ann N Y Acad Sci. 1964 Dec 28;121:404–427. doi: 10.1111/j.1749-6632.1964.tb14213.x. [DOI] [PubMed] [Google Scholar]
  8. FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
  9. HARVEY R. J., COLLINS E. B. Citrate transport system of Streptococcus diacetilactis. J Bacteriol. 1962 May;83:1005–1009. doi: 10.1128/jb.83.5.1005-1009.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HARVEY R. J., COLLINS E. B. ROLES OF CITRATE AND ACETOIN IN THE METABOLISM OF STREPTOCOCCUS DIACETILACTIS. J Bacteriol. 1963 Dec;86:1301–1307. doi: 10.1128/jb.86.6.1301-1307.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. HARVEY R. J., COLLINS E. B. Role of citritase in acetoin formation by Streptococcus diacetilactis and Leuconostoc citrovorum. J Bacteriol. 1961 Dec;82:954–959. doi: 10.1128/jb.82.6.954-959.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HIRSCH J., AHRENS E. H., Jr The separation of complex lipide mixtures by the use of silicic acid chromatography. J Biol Chem. 1958 Aug;233(2):311–20. [PubMed] [Google Scholar]
  13. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  14. SEIFTER S., DAYTON S. The estimation of glycogen with the anthrone reagent. Arch Biochem. 1950 Jan;25(1):191–200. [PubMed] [Google Scholar]
  15. Speckman R. A., Collins E. B. Diacetyl biosynthesis in Streptococcus diacetilactis and Leuconostoc citrovorum. J Bacteriol. 1968 Jan;95(1):174–180. doi: 10.1128/jb.95.1.174-180.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. THORNE K. J., KODICEK E. The metabolism of acetate and mevalonic acid by lactobacilli. I. The effect of acetate and mevalonic acid on growth. Biochim Biophys Acta. 1962 May 21;59:273–279. doi: 10.1016/0006-3002(62)90175-0. [DOI] [PubMed] [Google Scholar]
  17. WHITE D., KLEIN H. P. FACTORS AFFECTING FATTY ACID SYNTHESIS IN CELL-FREE PREPARATIONS FROM SACCHAROMYCES CEREVISIAE. Biochem Biophys Res Commun. 1965 Jun 18;20:78–84. doi: 10.1016/0006-291x(65)90953-8. [DOI] [PubMed] [Google Scholar]

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