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. 1971 Jun;21(6):1040–1045. doi: 10.1128/am.21.6.1040-1045.1971

Microorganisms in Unamended Soil as Observed by Various Forms of Microscopy and Staining 1

L E Casida Jr 1
PMCID: PMC377339  PMID: 4105130

Abstract

A light-diffraction microscope was modified to allow sequential viewing of the microorganisms in a soil smear by transmitted, reflected, and reflected-polarized incandescent light and by reflected ultraviolet light. Observations were also made by conventional incandescent and ultraviolet transmitted-light microscopy. All results for the various forms of bright-field microscopy with stained and unstained soils were in agreement, but they differed from the results obtained for two types of ultraviolet-fluorescence microscopy. The latter proved to be nonspecific for in situ soil microorganisms. Capsule-like areas were noted surrounding many of the resident microbial cells of soil when viewed by the various forms of bright-field microscopy. These areas could not be stained or removed by a variety of treatments, but they apparently often did take up stain after in situ soil growth had been initiated. It was concluded that these areas are not capsules but may represent a structural component of nonmultiplying microbial cells in soil.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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