Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1971 Jul;22(1):17–22. doi: 10.1128/am.22.1.17-22.1971

Comparison of Media for Isolation of Salmonellae and Shigellae from Fecal Specimens 1

Corwin Dunn 1,2, William J Martin 1,3
PMCID: PMC377369  PMID: 4999974

Abstract

Five transport media, eight plating media, and three enrichment broth media for the isolation of salmonellae and shigellae were evaluated. Eight laboratories in widely separated regions of the United States participated in this evaluation by submitting 490 fecal specimens in the transport media provided. The results suggest that the newer transport media may not offer any advantage over the use of buffered glycerol-saline in the isolation of these enteric pathogens. Shigellae were best isolated by direct inoculation, whereas salmonellae were isolated in greater numbers after tetrathionate (without Brilliant Green) enrichment with subsequent culturing on the plating medium. The use of a variety of plating media is recommended for the recovery of a larger number of these enteric pathogens.

Full text

PDF
18

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ARMSTRONG E. C. The relative efficacy of culture media in the isolation of Shigella sonnei. Mon Bull Minist Health Public Health Lab Serv. 1954 Apr;13:70–73. [PubMed] [Google Scholar]
  2. Amies C. R. A modified formula for the preparation of Stuart's Transport Medium. Can J Public Health. 1967 Jul;58(7):296–300. [PubMed] [Google Scholar]
  3. Borman E. K., Stuart C. A., Wheeler K. M. Taxonomy of the Family Enterobacteriaceae. J Bacteriol. 1944 Sep;48(3):351–367. doi: 10.1128/jb.48.3.351-367.1944. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CARY S. G., BLAIR E. B. NEW TRANSPORT MEDIUM FOR SHIPMENT OF CLINICAL SPECIMENS. I. FECAL SPECIMENS. J Bacteriol. 1964 Jul;88:96–98. doi: 10.1128/jb.88.1.96-98.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CROFT C. C., MILLER M. J. Isolation of Shigella from rectal swabs with Hajna GN broth. Am J Clin Pathol. 1956 Apr;26(4):411–417. doi: 10.1093/ajcp/26.4_ts.411. [DOI] [PubMed] [Google Scholar]
  6. GALTON M. M., HARDY A. V., MITCHELL R. B. The public health laboratory diagnosis of enteric infections. Am J Trop Med Hyg. 1950 Jan;30(1):77–90. doi: 10.4269/ajtmh.1950.s1-30.77. [DOI] [PubMed] [Google Scholar]
  7. Isenberg H. D., Kominos S., Siegel M. Isolation of Salmonellae and Shigellae from an artificial mixture of fecal bacteria. Appl Microbiol. 1969 Oct;18(4):656–659. doi: 10.1128/am.18.4.656-659.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. King S., Metzger W. I. A new plating medium for the isolation of enteric pathogens. I. hektoen enteric agar. Appl Microbiol. 1968 Apr;16(4):577–578. doi: 10.1128/am.16.4.577-578.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Morris G. K., Koehler J. A., Gangarosa E. J., Sharrar R. G. Comparison of media for direct isolation and transport of Shigellae from Fecal specimens. Appl Microbiol. 1970 Mar;19(3):434–437. doi: 10.1128/am.19.3.434-437.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rollender W., Beckford O., Belsky R. D., Kostroff B. Comparison of xylose lysine deoxycholate agar and MacConkey agar for the isolation of salmonella and shigella from clinical specimens. Tech Bull Regist Med Technol. 1969 Jan;39(1):8–10. [PubMed] [Google Scholar]
  11. STUART R. D. Transport medium for specimens in public health bacteriology. Public Health Rep. 1959 May;74(5):431–438. [PMC free article] [PubMed] [Google Scholar]
  12. THOMAS M. E. Disadvantages of the rectal swab in diagnosis of diarrhoea. Br Med J. 1954 Aug 14;2(4884):394–396. doi: 10.1136/bmj.2.4884.394. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Taylor W. I., Harris B. Isolation of shigellae. II. Comparison of plating media and enrichment broths. Am J Clin Pathol. 1965 Oct;44(4):476–479. [PubMed] [Google Scholar]
  14. Taylor W. I. Isolation of shigellae. I. Xylose lysine agars; new media for isolation of enteric pathogens. Am J Clin Pathol. 1965 Oct;44(4):471–475. [PubMed] [Google Scholar]
  15. Taylor W. I., Schelhart D. Isolation of Shigellae. VII. Comparison of gram-negative broth with Rappaport's enrichment broth. Appl Microbiol. 1969 Sep;18(3):393–395. doi: 10.1128/am.18.3.393-395.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Taylor W. I., Schelhart D. Isolation of shigellae. VI. Performance of media with stool speciments. Appl Microbiol. 1968 Sep;16(9):1387–1393. doi: 10.1128/am.16.9.1387-1393.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES