Skip to main content
Applied Microbiology logoLink to Applied Microbiology
. 1969 Jul;18(1):88–94. doi: 10.1128/am.18.1.88-94.1969

Coconut as a Medium for the Experimental Production of Aflatoxin

S N Arseculeratne 1,2, L M De Silva 1,2, S Wijesundera 1,2, C H S R Bandunatha 1,2
PMCID: PMC377895  PMID: 5803632

Abstract

Fresh, grated coconut has been found to be an excellent medium for aflatoxin production by Aspergillus flavus. Under optimal conditions, yields of 8 mg of total aflatoxin per g of substrate were obtained. Continuous agitation of the growth medium under moist conditions at 24 C produced highest yields. Aflatoxin was assayed both biologically and chromatographically. The aflatoxin content of cultures varied biphasically with the duration of incubation. It is suggested that this pattern could result from the sequential operation of factors promoting aflatoxin formation on the one hand and a detoxifying mechanism on the other.

Full text

PDF
93

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CHANG S. B., ABDEL-KADER M. M., WICK E. L., WOGAN G. N. AFLATOXIN B2: CHEMICAL IDENTITY AND BIOLOGICAL ACTIVITY. Science. 1963 Nov 29;142(3596):1191–1192. doi: 10.1126/science.142.3596.1191. [DOI] [PubMed] [Google Scholar]
  2. Ciegler A., Peterson R. E., Lagoda A. A., Hall H. H. Aflatoxin production and degradation by Aspergillus flavus in 20-liter fermentors. Appl Microbiol. 1966 Sep;14(5):826–833. doi: 10.1128/am.14.5.826-833.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Davis N. D., Diener U. L., Eldridge D. W. Production of aflatoxins B1 and G1 by Aspergillus flavus in a semisynthetic medium. Appl Microbiol. 1966 May;14(3):378–380. doi: 10.1128/am.14.3.378-380.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Davis N. D., Diener U. L. Growth and aflatoxin production by Aspergillus parasiticus from various carbon sources. Appl Microbiol. 1968 Jan;16(1):158–159. doi: 10.1128/am.16.1.158-159.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hesseltine C. W., Shotwell O. L., Ellis J. J., Stubblefield R. D. Aflatoxin formation by Aspergillus flavus. Bacteriol Rev. 1966 Dec;30(4):795–805. doi: 10.1128/br.30.4.795-805.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MATELES R. I., ADYE J. C. PRODUCTION OF AFLATOXINS IN SUBMERGED CULTURE. Appl Microbiol. 1965 Mar;13:208–211. doi: 10.1128/am.13.2.208-211.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Madhavan T. V., Gopalan C. The effect of dietary protein on carcinogenesis of aflatoxin. Arch Pathol. 1968 Feb;85(2):133–137. [PubMed] [Google Scholar]
  8. RICHARDSON L. R., WILKES S., GODWIN J., PIERCE K. R. Effect of moldy diet and moldy soybean meal on the growth of chicks and poults. J Nutr. 1962 Nov;78:301–306. doi: 10.1093/jn/78.3.301. [DOI] [PubMed] [Google Scholar]
  9. Schindler A. F., Palmer J. G., Eisenberg W. V. Aflatoxin Production by Aspergillus flavus as Related to Various Temperatures. Appl Microbiol. 1967 Sep;15(5):1006–1009. doi: 10.1128/am.15.5.1006-1009.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Schroeder H. W. Effect of corn steep liquor on mycelial growth and aflatoxin production in Aspergillus parasiticus. Appl Microbiol. 1966 May;14(3):381–385. doi: 10.1128/am.14.3.381-385.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Schroeder H. W., Hein H., Jr Aflatoxins: production of the toxins in vitro in relation to temperature. Appl Microbiol. 1967 Mar;15(2):441–445. doi: 10.1128/am.15.2.441-445.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Shotwell O. L., Hesseltine C. W., Stubblefield R. D., Sorenson W. G. Production of aflatoxin on rice. Appl Microbiol. 1966 May;14(3):425–428. doi: 10.1128/am.14.3.425-428.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Stubblefield R. D., Shotwell O. L., Hesseltine C. W., Smith M. L., Hall H. H. Production of aflatoxin on wheat and oats: measurement with a recording densitometer. Appl Microbiol. 1967 Jan;15(1):186–190. doi: 10.1128/am.15.1.186-190.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES