Abstract
MM virus propagated in mouse brain replicates to low titers in L cells without production of cytopathic effect (CPE). After growing the virus in BHK-21 cells, however, the virus replicates to high titers in L cells with complete CPE. It was found that suspensions of MM virus propagated in L cells directly from the mouse brain contained much more interferon than did suspensions of virus which had first been grown in BHK-21 cells. Mouse brain suspensions of the virus were also found to contain high interferon titers. Treatment of L cells with actinomycin D before infection with mouse brain-grown virus resulted in full virus replication with CPE. BHK-21 cell-grown virus diluted in L cell interferon behaved like mouse brain-grown virus in L cells. It is concluded that the presence of interferon in the inoculum is largely responsible for the suppression of MM virus replication in L cells.
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Selected References
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- Giron D. J., Pindak F. F. Propagation of MM virus in L cells. Appl Microbiol. 1969 Jun;17(6):811–814. doi: 10.1128/am.17.6.811-814.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HO M. IDENTIFICATION AND "INDUCTION" OF INTERFERON. Bacteriol Rev. 1964 Dec;28:367–381. doi: 10.1128/br.28.4.367-381.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JUNGEBLUT C. W., KODZA H. Studies on propagation of the Col SK group of viruses in various tissue culture media. Proc Soc Exp Biol Med. 1957 Oct;96(1):133–139. doi: 10.3181/00379727-96-23413. [DOI] [PubMed] [Google Scholar]
- Jungeblut C. W., Dalldorf G. Epidemiological and Experimental Observations on the Possible Significance of Rodents in a Suburban Epidemic of Poliomyelitis. Am J Public Health Nations Health. 1943 Feb;33(2):169–172. doi: 10.2105/ajph.33.2.169. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LEVINE S. EFFECT OF ACTINOMYCIN D AND PUROMYCIN DIHYDROCHLORIDE ON ACTION OF INTERFERON. Virology. 1964 Dec;24:586–588. doi: 10.1016/0042-6822(64)90211-9. [DOI] [PubMed] [Google Scholar]
- LEVY H. B., AXELROD D., BARON S. MESSENGER RNA FOR INTERFERON PRODUCTION. Proc Soc Exp Biol Med. 1965 Feb;118:384–385. doi: 10.3181/00379727-118-29850. [DOI] [PubMed] [Google Scholar]
- Pindak F. F., Schmidt J. P. Propagation of MM virus in continuous cell lines. Appl Microbiol. 1969 Jun;17(6):815–818. doi: 10.1128/am.17.6.815-818.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Taylor J. Inhibition of interferon action by actinomycin. Biochem Biophys Res Commun. 1964;14:447–451. doi: 10.1016/0006-291x(64)90084-1. [DOI] [PubMed] [Google Scholar]
- WAGNER R. R. INHIBITION OF INTERFERON BIOSYNTHESIS BY ACTINOMYCIN D. Nature. 1964 Oct 3;204:49–51. doi: 10.1038/204049a0. [DOI] [PubMed] [Google Scholar]