Abstract
To improve efficiency of isolation of tubercle bacilli from clinical specimens, the following recommendations are presented. (i) Employ multiple specimens consisting of a combination of morning sputums for the early detection of positives, along with 24-hr sputum pools for the greatest total yield of positives. (ii) When timing is rigorously controlled, Zephiran-trisodium phosphate and sodium hydroxide-acetylcysteine are comparable, but if timing cannot rigidly be controlled, employ the Zephiran-trisodium phosphate digestion procedure to allow the greatest freedom in exposure time with the lowest kill rate to tubercle bacilli. (iii) Employ both an agar medium incubated in 5% CO2, for the early detection of positives as well as positives in the presence of contaminants, and an egg medium, preferably with CO2, to increase the yield of positives.
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Selected References
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- KUBICA G. P., DYE W. E., COHN M. L., MIDDLEBROOK G. Sputum digestion and decontamination with N-acetyl-L-cysteine-sodium hydroxide for culture of mycobacteria. Am Rev Respir Dis. 1963 May;87:775–779. doi: 10.1164/arrd.1963.87.5.775. [DOI] [PubMed] [Google Scholar]
- SCHAEFER W. B., COHN M. L., MIDDLEBROOK G. The role of biotin and carbon dioxide in the cultivation of Mycobacterium tuberculosis. J Bacteriol. 1955 Jun;69(6):706–712. doi: 10.1128/jb.69.6.706-712.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wayne L. G., Krasnow I. Preparation of tuberculosis susceptibility testing mediums by means of impregnated disks. Am J Clin Pathol. 1966 Jun;45(6):769–771. doi: 10.1093/ajcp/45.6_ts.769. [DOI] [PubMed] [Google Scholar]