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. 1973 Sep;26(3):381–385. doi: 10.1128/am.26.3.381-385.1973

In Vitro Production of Clostridium perfringens Enterotoxin and Its Detection by Reversed Passive Hemagglutination

T Uemura 1,2, G Sakaguchi 1,2, H P Riemann 1,2
PMCID: PMC379795  PMID: 4356461

Abstract

The reversed passive hemagglutination (RPHA) test yielded a positive reaction in 2 h with as little as 0.5 ng of purified Clostridium perfringens enterotoxin (CPE) per ml as well as with cultures of some C. perfringens grown in Duncan-Strong (DS) medium. This method is the most sensitive, the simplest, and the fastest among all reported. The time course of CPE production of Clostridium perfringens NCTC 8798 in DS was investigated by RPHA. CPE in culture was detectable at 4 h, increased gradually, reached a maximum at 12 to 14 h, and remained at a high level of 20 μg/ml through 48 h of incubation. CPE synthesized within cells is released easily by sonic disruption of young cultures and by aging the cultures 20 h or more. Heat shock of the cell inoculum was essential for CPE production by C. perfringens in DS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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