Skip to main content
NCBI home page
Applied Microbiology logoLink to Applied Microbiology
. 1973 Dec;26(6):904–913. doi: 10.1128/am.26.6.904-913.1973

Recovery and Identification of Anaerobes: a System Suitable for the Routine Clinical Laboratory

Paul D Ellner 1,2, Paul A Granato 1,2, Carolyn B May 1,2
PMCID: PMC379932  PMID: 4588198

Abstract

A system for the isolation of anaerobes based upon the use of reducible solid media is described. Plates of reducible media prepared and stored aerobically were reduced before use by incubation in a GasPak jar for 24 h. Clinical specimens for culture were carefully selected. The value of Amies transport medium was confirmed. Selective and nonselective formulations of reducible media were used for primary isolation. Abbreviated identification schemes based in part on gas-liquid chromatography are presented. The suitability of this system for improving the recovery and identification of anaerobes in a routine clinical laboratory is documented.

Full text

PDF
904

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arank A., Syed S. A., Kenney E. B., Freter R. Isolation of anaerobic bacteria from human gingiva and mouse cecum by means of a simplified glove box procedure. Appl Microbiol. 1969 Apr;17(4):568–576. doi: 10.1128/am.17.4.568-576.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barry A. L., Fay G. D., Sauer R. L. Efficiency of a transport medium for the recovery of aerobic and anaerobic bacteria from applicator swabs. Appl Microbiol. 1972 Jul;24(1):31–33. doi: 10.1128/am.24.1.31-33.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Ellner P. D., O'Donnell D. A selective differential medium for histotoxic Clostridia. Am J Clin Pathol. 1971 Aug;56(2):197–200. doi: 10.1093/ajcp/56.2.197. [DOI] [PubMed] [Google Scholar]
  4. Ellner P. D. System for inoculation of blood in the laboratory. Appl Microbiol. 1968 Dec;16(12):1892–1894. doi: 10.1128/am.16.12.1892-1894.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Martin W. J. Practical method for isolation of anerobic bacteria in the clinical laboratory. Appl Microbiol. 1971 Dec;22(6):1168–1171. doi: 10.1128/am.22.6.1168-1171.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. McMinn M. T., Crawford J. J. Recovery of anaerobic microorganisms from clinical specimens in prereduced media versus recovery by routine clinical laboratory methods. Appl Microbiol. 1970 Feb;19(2):207–213. doi: 10.1128/am.19.2.207-213.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Moore W. B. Solidified media suitable for the cultivation of Clostridium novyi type B. J Gen Microbiol. 1968 Oct;53(3):415–423. doi: 10.1099/00221287-53-3-415. [DOI] [PubMed] [Google Scholar]
  8. Morello J. A., Ellner P. D. New medium for blood cultures. Appl Microbiol. 1969 Jan;17(1):68–70. doi: 10.1128/am.17.1.68-70.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Rosenblatt J. E., Fallon A., Finegold S. M. Comparison of methods for isolation of anaerobic bacteria from clinical specimens. Appl Microbiol. 1973 Jan;25(1):77–85. doi: 10.1128/am.25.1.77-85.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES