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. 1974 Jan;27(1):102–106. doi: 10.1128/am.27.1.102-106.1974

Purification on Renografin Density Gradients of Chlamydia trachomatis Grown in the Yolk Sac of Eggs

Lawrence Howard 1,2, Neil S Orenstein 1,2, Norval W King 1,2
PMCID: PMC379975  PMID: 4855645

Abstract

Chlamydia trachomatis grown in the yolk sac of embryonated eggs was purified by centrifugation on continuous isopycnic Renografin density gradients. A band of chlamydial particles with a buoyant density of 1.20 contained 70% of the starting particles, and electron microscopy revealed the virtual absence of contaminating egg material. Centrifugation on Renografin gradients caused only a moderate decrease in infectivity. For large-scale purification, infected yolk sac was centrifuged through Renografin solutions, resulting in greater than 60% recovery of starting chlamydial particles, but less than 1% recovery of the dry weight and protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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