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. 1974 May;27(5):985–987. doi: 10.1128/am.27.5.985-987.1974

A Serum Bottle Modification of the Hungate Technique for Cultivating Obligate Anaerobes

Terry L Miller 1,2, M J Wolin 1,2
PMCID: PMC380188  PMID: 4598231

Abstract

The modification described utilizes a serum bottle closed with a butyl rubber stopper with a crimped metal seal for the growth of obligately anaerobic bacteria.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bryant M. P. Commentary on the Hungate technique for culture of anaerobic bacteria. Am J Clin Nutr. 1972 Dec;25(12):1324–1328. doi: 10.1093/ajcn/25.12.1324. [DOI] [PubMed] [Google Scholar]
  2. Macy J. M., Snellen J. E., Hungate R. E. Use of syringe methods for anaerobiosis. Am J Clin Nutr. 1972 Dec;25(12):1318–1323. doi: 10.1093/ajcn/25.12.1318. [DOI] [PubMed] [Google Scholar]
  3. Miller T. L., Wolin M. J. Formation of hydrogen and formate by Ruminococcus albus. J Bacteriol. 1973 Nov;116(2):836–846. doi: 10.1128/jb.116.2.836-846.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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