Abstract
A detection procedure was developed in which a newly devised lysine-iron medium was used as a one-step selective and enrichment medium for detection of salmonellae by the fluorescent-antibody technique. Incubation was conducted in two steps: initially at 30 C for 5 hr to resuscitate sublethally stressed cells, followed by incubation at 39 C for 17 hr. Twenty-seven strains of salmonellae from groups A-I were utilized in the development of this procedure which was sensitive enough to detect one Salmonella bacterium in 100 g of nonfat dry milk.
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Selected References
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- BACHRACH U. An improved method for the determination of lysine decarboxylase activity of Salmonellae. Am J Clin Pathol. 1959 Dec;32:580–581. doi: 10.1093/ajcp/32.6_ts.580. [DOI] [PubMed] [Google Scholar]
- Banwart G. J. Glassware apparatus for determining motile bacteria. 1. Salmonella. Poult Sci. 1968 Jul;47(4):1209–1212. doi: 10.3382/ps.0471209. [DOI] [PubMed] [Google Scholar]
- EDWARDS P. R., FIFE M. A. Lysine-iron agar in the detection of Arizona cultures. Appl Microbiol. 1961 Nov;9:478–480. doi: 10.1128/am.9.6.478-480.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FALKOW S. Activity of lysine decarboxlase as an aid in the identification of Salmonellae and Shigellae. Am J Clin Pathol. 1958 Jun;29(6):598–600. doi: 10.1093/ajcp/29.6_ts.598. [DOI] [PubMed] [Google Scholar]
- GEORGALA D. L., BOOTHROYD M. A RAPID IMMUNOFLUORESCENCE TECHNIQUE FOR DETECTING SALMONELLAE IN RAW MEAT. J Hyg (Lond) 1964 Sep;62:319–327. doi: 10.1017/s0022172400040031. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Insalata N. F., Schulte S. J., Berman J. H. Immunofluorescence technique for the detection of salmonellae in various foods. Appl Microbiol. 1967 Sep;15(5):1145–1149. doi: 10.1128/am.15.5.1145-1149.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reamer R. H., Hargrove R. E., McDonough F. E. Increased sensitivity of immunofluorescent assay for Salmonella in nonfat dry milk. Appl Microbiol. 1969 Sep;18(3):328–331. doi: 10.1128/am.18.3.328-331.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sperber W. H., Deibel R. H. Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions. Appl Microbiol. 1969 Apr;17(4):533–539. doi: 10.1128/am.17.4.533-539.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tittsler R. P. The Effect of Temperature upon the Production of Hydrogen Sulphide by Salmonella pullorum. J Bacteriol. 1931 Feb;21(2):111–118. doi: 10.1128/jb.21.2.111-118.1931. [DOI] [PMC free article] [PubMed] [Google Scholar]