Transferability and fine-mapping of glucose and insulin quantitative trait loci across populations: CARe, the Candidate Gene Association Resource

C-T Liu; M C Y Ng; D Rybin; A Adeyemo; S J Bielinski; E Boerwinkle; I Borecki; B Cade; Y D I Chen; L Djousse; M Fornage; M O Goodarzi; S F A Grant; X Guo; T Harris; E Kabagambe; J R Kizer; Y Liu; K L Lunetta; K Mukamal; J A Nettleton; J S Pankow; S R Patel; E Ramos; L Rasmussen-Torvik; S S Rich; C N Rotimi; D Sarpong; D Shriner; M Sims; J M Zmuda; S Redline; W H Kao; D Siscovick; J C Florez; J I Rotter; J Dupuis; J G Wilson; D W Bowden; J B Meigs

doi:10.1007/s00125-012-2656-4

. Author manuscript; available in PMC: 2013 Nov 1.

Published in final edited form as: Diabetologia. 2012 Aug 16;55(11):2970–2984. doi: 10.1007/s00125-012-2656-4

Transferability and fine-mapping of glucose and insulin quantitative trait loci across populations: CARe, the Candidate Gene Association Resource

C-T Liu ¹, M C Y Ng ², D Rybin ³, A Adeyemo ⁴, S J Bielinski ⁵, E Boerwinkle ⁶, I Borecki ⁷, B Cade ⁸, Y D I Chen ⁹, L Djousse ¹⁰, M Fornage ¹¹, M O Goodarzi ¹², S F A Grant ¹³, X Guo ¹⁴, T Harris ¹⁵, E Kabagambe ¹⁶, J R Kizer ¹⁷, Y Liu ¹⁸, K L Lunetta ¹⁹, K Mukamal ²⁰, J A Nettleton ²¹, J S Pankow ²², S R Patel ²³, E Ramos ²⁴, L Rasmussen-Torvik ²⁵, S S Rich ²⁶, C N Rotimi ²⁷, D Sarpong ²⁸, D Shriner ²⁹, M Sims ³⁰, J M Zmuda ³¹, S Redline ³², W H Kao ³³, D Siscovick ³⁴, J C Florez ³⁵, J I Rotter ³⁶, J Dupuis ³⁷, J G Wilson ³⁸, D W Bowden ³⁹, J B Meigs ⁴⁰

¹Department of Biostatistics, Boston University School of Public Health, Boston, MA, USA

²Center for Genomics and Personalized Medicine Research, Center for Diabetes Research, Wake Forest University School of Medicine, Winston-Salem, NC, USA

³Boston University Data Coordinating Center, Boston, MA, USA

⁴National Human Genome Research Institute, Bethesda, MD, USA

⁵Mayo Clinic, Rochester, MN, USA

⁶University of Texas Health Science Center at Houston, Houston, TX, USA

⁷Washington University, St Louis, MO, USA

⁸Brigham and Women's Hospital, Boston, MA, USA

⁹Cedars Sinai Medical Center, Los Angeles, CA, USA

¹⁰Brigham and Women's Hospital, Boston, MA, USA; Department of Medicine, Harvard Medical School, Boston, MA, USA; Boston VA Healthcare System, Boston, MA, USA;

¹¹University of Texas Health Science Center at Houston, Houston, TX, USA

¹²Cedars Sinai Medical Center, Los Angeles, CA, USA

¹³Children's Hospital of Philadelphia, Philadelphia, PA, USA

¹⁴Cedars Sinai Medical Center, Los Angeles, CA, USA

¹⁵National Institute on Aging, Bethesda, MD, USA

¹⁶University of Alabama, Birmingham, AL, USA

¹⁷Cornell University, New York, NY, USA

¹⁸Center for Genomics and Personalized Medicine Research, Center for Diabetes Research, Wake Forest University School of Medicine, Winston-Salem, NC, USA; Department of Epidemiology and Prevention, Wake Forest University, Winston-Salem, North Carolina, USA

¹⁹Department of Biostatistics, Boston University School of Public Health, Boston, MA, USA; National Heart, Lung, and Blood Institute' Framingham Heart Study, Framingham, MA, USA

²⁰Department of Medicine, Harvard Medical School, Boston, MA, USA

²¹University of Texas Health Science Center at Houston, Houston, TX, USA

²²University of Minnesota, Minneapolis, MN, USA

²³Brigham and Women's Hospital, Boston, MA, USA

²⁴National Human Genome Research Institute, Bethesda, MD, USA

²⁵Northwestern University, Chicago, IL, USA

²⁶University of Virginia, Charlottesville, VA, USA

²⁷National Human Genome Research Institute, Bethesda, MD, USA

²⁸Jackson State University, Jackson, MS, USA

²⁹National Human Genome Research Institute, Bethesda, MD, USA

³⁰University of Mississippi Medical Center, Jackson, MS, USA

³¹University of Pittsburgh, Graduate School of Public Health, Pittsburgh, PA, USA

³²Brigham and Women's Hospital, Boston, MA, USA

³³Johns Hopkins University, Baltimore, MD, USA

³⁴University of Washington, Seattle, WA, USA

³⁵Department of Medicine, Harvard Medical School, Boston, MA, USA; Diabetes Unit and Center for Human Genetic Research, Massachusetts General Hospital, Boston, MA, USA; Program in Medical and Population Genetics, Broad Institute, Cambridge, MA, USA

³⁶Cedars Sinai Medical Center, Los Angeles, CA, USA

³⁷Department of Biostatistics, Boston University School of Public Health, Boston, MA, USA; National Heart, Lung, and Blood Institute's Framingham Heart Study, Framingham, MA, USA

³⁸University of Mississippi Medical Center, Jackson, MS, USA

³⁹Center for Genomics and Personalized Medicine Research, Center for Diabetes Research, Wake Forest University School of Medicine, Winston-Salem, NC, USA; Departments of Biochemistry and Internal Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USA

⁴⁰Department of Medicine, Harvard Medical School, Boston, MA, USA; General Medicine Division, Massachusetts General Hospital, 50 Staniford Street, 9th Flr, Boston, MA, USA

^✉

Corresponding author: J. B. Meigs, jmeigs@partners.org

PMCID: PMC3804308 NIHMSID: NIHMS400966 PMID: 22893027

Abstract

Aims/hypothesis

Hyperglycaemia disproportionately affects African-Americans (AfAs). We tested the transferability of 18 single-nucleotide polymorphisms (SNPs) associated with glycaemic traits identified in European ancestry (EuA) populations in 5,984 non-diabetic AfAs.

Methods

We meta-analysed SNP associations with fasting glucose (FG) or insulin (FI) in AfAs from five cohorts in the Candidate Gene Association Resource. We: (1) calculated allele frequency differences, variations in linkage disequilibrium (LD), fixation indices (F_sts) and integrated haplotype scores (iHSs); (2) tested EuA SNPs in AfAs; and (3) interrogated within ±250 kb around each EuA SNP in AfAs.

Results

Allele frequency differences ranged from 0.6% to 54%. F_st exceeded 0.15 at 6/16 loci, indicating modest population differentiation. All iHSs were <2, suggesting no recent positive selection. For 18 SNPs, all directions of effect were the same and 95% CIs of association overlapped when comparing EuA with AfA. For 17 of 18 loci, at least one SNP was nominally associated with FG in AfAs. Four loci were significantly associated with FG (GCK, p=5.8 × 10^-8; MTNR1B, p=8.5 × 10^-9; and FADS1, p=2.2 × 10^-4) or FI (GCKR, p=5.9 × 10^-4). At GCK and MTNR1B the EuA and AfA SNPs represented the same signal, while at FADS1, and GCKR, the EuA and best AfA SNPs were weakly correlated (r²<0.2), suggesting allelic heterogeneity for association with FG at these loci.

Conclusions/interpretation

Few glycaemic SNPs showed strict evidence of transferability from EuA to AfAs. Four loci were significantly associated in both AfAs and those with EuA after accounting for varying LD across ancestral groups, with new signals emerging to aid fine-mapping.

Keywords: African ancestry, Genetics, Genome-wide association, LD mapping, Minorities, Type 2 diabetes

In the USA, over 23 million people have type 2 diabetes, but minority groups such as African-Americans (AfAs) are disproportionately affected. In 2005, the prevalence of type 2 diabetes was about 28% higher in AfAs than in individuals of European ancestry (EuA), and risk for diabetes (defined by either elevated fasting glucose or HbA_1c) was about twice as high. By 2050, this racial disparity is projected to increase even further [1]. Racial/ethnic variation in health-related behaviours and obesity accounts for a lot of this disparity [2, 3], but genetic factors could also contribute [4-6].

Until recently, the majority of genetic studies of type 2 diabetes and related quantitative traits (e.g. levels of fasting glucose [FG] and fasting insulin [FI]) have been conducted in EuA populations [7, 8]. There are strong scientific reasons to expand diabetes-related genetic analyses to AfA, quite apart from the disparity caused by their exclusion from this important research frontier. No single ancestral population is sufficient to fully uncover variants contributing to disease in humans, and of all continental ancestral groups, those of African ancestry contain the richest range of genetic variation. Trans-ethnic genetic analyses can elucidate ancestral differences in common risk-allele frequencies, linkage disequilibrium (LD) patterns and variant effect size differences, as well as guide locus fine-mapping to uncover functional variants identified in EuA genetic studies [4-6, 9, 10].

Study of AfA populations is likely to be especially important to identify diabetes-related genetic variants [4, 11]. Although one recent study examined whether type 2 diabetes associations found in EuA were also associated in about 2,500 AfA individuals [12], analyses of transferability of type-2-diabetes-associated variants from EuA to AfA populations have not so far been especially instructive, probably because of limited sample sizes and testing of tagging variants rather than functional variants in the setting of reduced LD [13-16]. On the other hand, analyses of continuously distributed quantitative traits such as FG or FI, elevations of which herald type 2 diabetes [17], offer the advantage of increased power and reduced misclassification by disease status [18]. One recent study of 927 unrelated AfAs confirmed the association of six EuA-identified variants with FG [19]. Another study of 3024 adults reported that allele frequencies of 16 novel FG-associated single-nucleotide polymorphisms (SNPs) varied across non-Hispanic whites, non-Hispanic blacks and Mexican Americans [9]. In the present study, we investigated whether testing associations of EuA-identified variants in the large Candidate Gene Association Resource (CARe) study would confirm wide risk-allele frequency variation and LD differences in AfA vs EuA and show limited EuA-associated SNP associations in AfA, but when accounting for LD, would show that most FG- or FI-associated loci are associated with diabetes-related quantitative traits in AfAs at different, generally independent, variants.

Methods

Candidate Gene Association Resource study samples

The Candidate Gene Association Resource (CARe) is a National Heart, Lung, and Blood Institute (NHLBI) shared resource for genotype association analyses comprising five parent studies with AfA participants and traits of interest. All individuals provided written informed consent for DNA studies as part of their parent study. CARe study samples are described in greater detail in Lettre et al [15]. Briefly, the studies were as follows.

Atherosclerosis Risk in Communities

The Atherosclerosis Risk in Communities (ARIC) study is an ongoing prospective cohort study originally designed to investigate risk factors of subclinical and clinical atherosclerosis. It includes measurement of cardiovascular and diabetes risk factors. ARIC enrolled 15,792 participants, aged 45-64 years, from four field centres, and has been described in detail elsewhere [20]. This study includes only self-reported AfA participants, and all participants provide written informed consent.

Coronary Artery Risk Development in young Adults

The Coronary Artery Risk Development in young Adults (CARDIA) study is a prospective multicentre investigation of the development and determinants of clinical and subclinical cardiovascular disease (CVD) and its risk factors. The participants were recruited from four sites and appropriate informed consent was provided [21].

Cleveland Family Study

The Cleveland Family Study (CFS) is a family-based longitudinal study originally designed to investigate genetic and non-genetic risk factors for sleep apnoea [22]. For this analysis, only data from AfAs participating in the most recent examination that included a morning fasting blood draw were analysed. All participants provided written informed consent.

Jackson Heart Study

The Jackson Heart Study (JHS) is a prospective population-based study of CVD among non-institutionalised AfA adults aged 21–95 years and residing in the Jackson, MS metropolitan statistical area (MSA) [23]. The final JHS cohort included 5,301 participants, equivalent to 7% of all AfAs aged 21–95 residing in the Jackson MSA¹⁷. Details of the study design and recruitment protocol have been described elsewhere [23-26]. Appropriate informed consent was provided.

Multi-Ethnic Study of Atherosclerosis

The Multi-Ethnic Study of Atherosclerosis (MESA) is a community-based cohort study designed to investigate characteristics and risk factors for subclinical CVD. Participants were recruited from six centres and appropriate informed consent was provided. From 2000 to 2002, 6,814 participants (38% non-Hispanic white, 28% black, 12% Chinese, 22% Hispanic; aged 45–84 years) without existing clinical CVD were enrolled [27]. Only data from AfA individuals were included in this analysis.

In all studies, FG was measured using hexokinase (in mmol/l) and FI was measured using RIA or ELISA methods in serum or plasma (in μU/ml, converted into pmol/l using a conversion factor of 6).

Genotyping, imputation and principal components analysis

All samples were genotyped at the Broad Institute using the Affymetrix Genome-Wide Human SNP Array 6.0 (Affy6.0, Santa Clara, CA, USA). Quality-control steps were performed centrally by the CARe analytical group at the Broad Institute [15]. Imputation was conducted centrally at the Broad Institute with MACH 1.0.16 using the HapMap 2 reference panel of Utah residents of northern and western European ancestry (CEU) and the Yoruba in Ibadan, Nigeria (YRI). The allelic concordance rate, defined as 1–1/2*|imputed_dosage – genotyped_dosage| based on the CEU+YRI panel, was 95.6%, comparable with rates for individuals of African descent imputed with HapMap 2 YRI individuals [15]. Filters applied to the imputation data were based on the imputation quality measure RSQ_HAT with a threshold of >0.3 and a minor allele frequency threshold of >0.01. Ancestry principal components (PCs) were generated using EIGENSTRAT (http://genepath.med.harvard.edu/∼reich/EIGENSTRAT.htm).

Allele frequency differences and natural selection

We used several approaches to evaluate population differentiation and natural selection at index SNPs. First, we assessed index SNP allele-frequency differences in CARe comparing individuals of EuA vs AfA by calculating the absolute value of (index-risk-allele frequency in EuA – index-risk-allele frequency in AfA), using the trait-raising allele in EuA as the risk allele. Second, we calculated Wright's fixation index (F_st) to assess the degree of population differentiation as reflected by divergent allele frequencies. We calculated F_st using CARe risk-allele frequencies for AfAs, and the Meta-Analyses of Glucose and Insulin-related traits Consortium (MAGIC) [8] risk-allele frequencies for those of EuA, but because the EuA sample size (46,186) was about seven times the AfA sample size, potentially obscuring population differentiation at any locus, we also calculated the F_st using CEU and YRI HapMap 2 data for which the sample sizes are similar. Third, we calculated the integrated haplotype score (iHS) in HapMap 2 data to assess evidence for recent positive selection at the index SNP. F_st and iHS were calculated using Haplotter [28]. We used varLD (A program for quantifying variation in linkage disequilibrium patterns between populations) to compare EuA vs AfA regional LD patterns. The approach quantifies genomic regions carrying dissimilar patterns of LD, regions at which different haplotypes, structural variants and signs of positive selection may be particularly common [29].

Statistical analysis to generate SNP-trait-association results

We tested SNP associations with quantitative traits in each cohort according to a uniform protocol. The primary study traits were FG and FI; in secondary analyses we also tested associations with HOMA-insulin resistance (HOMA-IR) and HOMA-beta cell function (HOMA-B) [30]. FI and HOMA were analysed on the natural log scale. Ancestry PCs were created in each study; as just the first two PCs were associated with FG (p<0.005) these were used as covariates in each study to control population stratification. Genomic control correction based on median χ² value was calculated across all loci in the genome-wide association study (GWAS) data within each study to adjust for inflation of test statistics. Traits were analysed using linear regression under an additive genetic model, adjusted for age, age², sex, study site (if applicable) and the first two PCs; relatedness was accounted for when necessary using linear mixed effect (LME) models. PLINK (http://pngu.mgh.harvard.edu/∼purcell/PLINK/) and R (v2.9.0) were used for data management and statistical analyses. We performed fixed-effect meta-analyses on study-specific genome-wide association results to generate a combined association effect estimate for each SNP using the inverse-variance weighted approach in METAL (a tool for meta-analysis genome-wide association scans) [31]. Nominal significance was defined as a SNP association p value < 0.05. We estimated the power of the sample to detect additive SNP-FG (in mmol/l) associations, using the detectable effect size as the 25th, 50th and 75th percentiles of the distribution of β coefficients seen in Dupuis et al [8]. At n∼6,000 and α= 4.05 × 10^-5, we had low power to detect all but the largest effect sizes (electronic supplementary material [ESM] Fig. 1).

Fig. 1 — 500 kb regional association plots centred at the index SNPs identified from EuA samples at *GCK*, *MTNR1B*, *FADS1* and *G6PC2*. The x-axis denotes genomic position and the y-axis denotes the –log(p value) for each SNP. The two larger-sized data points represent the best SNPs within the region in the AfA CARe sample (black arrows) and the index SNPs (white arrows, locating the centre of the region) previously reported from EuA population samples. The colour of each data point indicates its LD value (r²) with the index SNP based on HapMap 2 YRI: white, r²=0.0–0.2; yellow, r²=0.2–0.5; orange, r²=0.5–0.8; red, r²=0.8–1.0. The blue line represents the recombination rate. The green line shows the varLD score at each SNP and is highlighted with dark brown if the varLD score is ≥95th percentile of the genome-wide varLD score, comparing LD information between YRI and CEU HapMap 2 samples [29]. (a)*GCK* region: the index SNP rs4607517 has a p value of 1.42 × 10^-6 in AfA CARe individuals, while the best SNP rs1799884 has a p value of 5.79 × 10^-8. Their r² values are 0.469 in HapMap 2 YRI and 1.0 in HapMap 2 CEU. (b) *MTNR1B* region: rs10830963 is the index SNP and the best SNP within the region in AfA CARe individuals, with a p value of 8.51 × 10^-9. (c) *G6PC2* region: the index SNP rs560887 has a p value of 4.08 × 10^-3 in AfA CARe individuals and the best SNP rs853789 has a p value of 1.62 × 10^-3. The r² value is not known in HapMap 2 YRI and is 0.692 in HapMap 2 CEU. A known functional SNP in the region, rs13431652 [38], has r²=0.923 with rs560887 and r²=0.595 with rs853789 in HapMap 2 CEU, but in HapMap 2 YRI the r² of rs13431652 with rs853789 is 0.004. (d) *FADS1* region: the index SNP rs174550 has a p value of 0.399 in AfA CARe individuals and the best SNP rs7937198 has a p value of 2.22 × 10^-4. Their r² values are 0.00 in HapMap 2 YRI and 0.24 in HapMap 2 CEU

Interrogation of transferability

We defined SNP transferability as an EuA index SNP having the same direction of association with the quantitative trait and a p value <0.05 in AfA individuals, and locus transferability as a locus containing any SNPs significantly associated with the quantitative trait in AfAs, with significance defined as any SNP with a p value <0.05 after accounting for the effective number of tests at the locus. We took two approaches to investigate transferability of EuA quantitative trait-associated SNPs and loci in AfA. First, we tested index SNPs reported to be associated with FG or FI at genome-wide significance (5 × 10^-8) in EuA individuals in the MAGIC study [8]. We also tested replicated significant type 2 diabetes SNPs [32-36] that had consistent nominal associations with FG or FI in the Diabetes Genetics Replication and Meta-analysis Consortium (DIAGRAM) or MAGIC studies [7, 8]. Second, we searched for the best SNPs in AfAs accounting for differences in regional LD structure in AfAs vs those with EuA by interrogating the flanking 250 kb regions on each side of the index SNP, with the best SNP defined as the SNP with the smallest FG or FI association p value in the region in AfA individuals. Note that we treated each region independently in this follow-up study of regions that had a higher prior probability of association with the trait of interest than the genomic average, based on previously reported associations in Europeans. As the results for any one region had no inferential bearing on the results for any other region, we made region-wide (not genome-wide) corrections to adjust for multiple comparisons. For a locus, the significance of the identified best SNPs was evaluated using a Bonferroni correction adjusting for the effective number of tests within that locus, estimated using the Li and Ji algorithm [37].

Results

We analysed data from 5,984 (FG) and 5,969 (FI) non-diabetic AfA individuals from five CARe participating cohorts (Table 1). About 40% within each cohort were men and cohorts had a similar mean FG levels (range from 4.5 to 5.4 mmol/l). Mean age ranged more widely (from 24.4 to 61.7 years) across cohorts, as did mean FI (from 40.6 to 97.8 pmol/l).

Table 1. Characteristics of five CARe cohorts.

Cohort	ARIC (n=1734)	CARDIA (n=930)	CFS (n=272)	JHS (n=1707)	MESA (n=1341)
Men, n (%)	649 (37.4)	362 (38.9)	104 (38.2)	682 (40.0)	596 (44.4)
Age (years)	53±5.8	24±3.9	36±17.7	49±12.1	62±10.3
FG (mmol/l)	5.4±0.5	4.5±0.48	5.1±0.54	5±0.5	5±0.6
FI (pmol/l)^a	89.1±65.7	75.1±52.7	84.2±69.5	97.8±55.6	40.6±27.8
HOMA-B^b	160.7±110.1	290.6±248.3	187.4±193.1	229.9±129.7	95.8±65.6
HOMA-IR^b	3.6±2.9	2.6±1.9	3.3±2.8	3.7±2.4	1.6±1.2

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Data are mean±SD unless otherwise specified

1 pmol/l=6 μU/ml for insulin

Numbers of missing individuals for HOMA-B and HOMA-IR: ARIC, 9; CARDIA, 17; CFS, 1; JHS, 0; MESA, 4

Allele frequency differences and natural selection

As observed for quantitative glycaemic traits, index SNP allele-frequency differences between those of EuA and AfAs had a wide range (Table 2). F_st exceeded 0.15 at 1/18 loci in CARe but at 6/18 loci in HapMap 2, indicating modest population differentiation based on allele frequency differences at ADRA2A, CRY2, PROX1, FADS1, IGF1 and C2CD4B. The absolute values of all iHSs were <2, suggesting no recent strong positive selection at any locus. For the FADS1 and G6PC2 loci at which the index and best SNPs were weakly correlated, the varLD scores were elevated in AfAs, suggesting allelic heterogeneity of tag SNPs for causal variants or other forms of variation, such as copy number variations (Fig. 1).

Table 2. Allele frequency differences, F_sts and iHSs in AfA and EuA populations.

Nearest gene(s)	SNP	Alleles^a	EUA EAF^b	AfA EAF^b	CARe (EAF EuA– AfA)	p value for EAF difference^c	F_st CARe	EAF HapMap 2 CEU	EAF HapMap 2 YRI	F_st HapMap 2 CEU vs YRI	his HapMap 2 CEU	his HapMap 2 YRI
FG-associated loci
PROX1	rs340874	C/T	0.520	0.169	0.351	< 1 × 10^-384	0.051	0.508	0.092	0.207	−0.06	−0.09
G6PC2	rs560887	C/T	0.700	0.943	0.243	< 1 × 10^-384	0.031	0.700	1.000	−	−0.26	−
GCKR	rs780094	C/T	0.620	0.817	0.197	8.78 × 10^-196	0.017	0.617	0.883	0.095	−0.39	0.14
ADCY5	rs11708067	A/G	0.780	0.843	0.063	3.68 × 10^-29	0.002	0.783	0.917	0.035	−0.36	−0.79
SLC2A2	rs11920090	T/A	0.870	0.645	0.225	< 1 × 10^-384	0.040	0.853	0.667	0.051	1.17	0.60
GCK	rs4607517	A/G	0.160	0.101	0.059	6.49 × 10^-33	0.003	0.200	0.059	0.045	−0.26	−0.20
DGKB-TMEM195	rs2191349	T/G	0.520	0.593	0.073	1.60 × 10^-26	0.002	0.467	0.593	0.016	−0.13	−0.07
SLC30A8	rs13266634	C/T	0.680	0.908	0.228	2.96 × 10^-290	0.026	0.750	0.942	0.070	−1.87	−0.55
GLIS3	rs7034200	A/C	0.490	0.630	0.140	3.79 × 10^-92	0.008	0.525	0.585	0.003	0.47	−0.24
ADRA2A	rs10885122	G/T	0.870	0.334	0.537	< 1 × 10^-384	0.191	0.900	0.217	0.484	−0.23	−0.25
TCF7L2	rs7903146	T/C	0.290	0.284	0.006	3.20 × 10^-1	0.000	0.250	0.292	0.002	0.09	−0.27
MTNR1B	rs10830963	G/C	0.300	0.073	0.227	< 1 × 10^-384	0.027	0.300	0.050	0.108	−0.05	−0.55
MADD	rs7944584	A/T	0.750	0.950	0.200	2.27 × 10 ^-254	0.024	0.692	1.000	−	−0.31	−
FADS1	rs174550	T/C	0.640	0.916	0.276	< 1 × 10^-384	0.036	0.633	0.983	0.198	−0.62	−
CRY2	rs11605924	A/C	0.490	0.867	0.377	< 1 × 10^-384	0.059	0.542	0.941	0.209	−1.50	−1.02
C2CD4B	rs11071657	A/G	0.630	0.868	0.238	4.61 × 10^-292	0.026	0.592	0.942	0.171	1.07	−1.65
FI-associated loci
GCKR	rs780094	C/T	0.620	0.817	0.197	2.70 × 10^-192	0.020	0.617	0.883	0.095	−0.39	0.14
IGF1	rs35767	G/A	0.850	0.570	0.280	< 1 × 10^-384	0.061	0.900	0.525	0.172	−1.290	0.374

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The loci in this table were obtained from Dupuis et al (2010) [8]

Effect (trait-raising)/other allele

EAF, effect allele frequency

P-value for testing whether the effect allele frequency EAF in AfAs is the same as the effect allele frequency in EuA populations

Interrogation of EuA FG- and FI-associated loci in AfAs

Trait-raising allele frequencies of EuA index SNPs in AfAs varied widely, from a minimum of a 0.6% difference (TCF7L2) up to a 54% difference (ADRA2) between ancestry groups (Table 3). Of EuA index SNPs tested directly in AfAs, all 18 were directionally consistent; three of the 18 were nominally (p<0.05) associated with FG and one with FI. In addition, all of the published 95% CIs around point estimates for association of index SNPs in those of EuA overlapped with those tested here in AfAs. We also performed formal heterogeneity tests comparing effects in EuAs with effects in AfAs. Heterogeneity tests yielded only one nominally significant result for rs10830963 (p value = 4.26 × 10^-2 at MTNR1B), indicating that the effect sizes in those of EuA vs AfAs for at least 17 index SNPs were not statistically different.

Table 3. Transferability to AfAs of FG- and FI-related loci previously reported in EuA populations.

Nearest gene(s)	Index SNP	Chr.	Position	Alleles^b	EuA association			AfA association for index SNP				Heterogeneity^a

					EAF	Effect ([mmol/l] per effect allele)	95% CI	EAF	Effect ([mmol/l] per effect allele)	95% CI	p value	p value
FG-associated loci
PROX1	rs340874	1	212225879	C/T	0.520	0.013	0.007, 0.019	0.169	0.006	−0.020, 0.031	6.63 × 10^-1	6.00 × 10 ¹
G6PC2	rs560887	2	169471394	C/T	0.700	0.075	0.069, 0.081	0.943	0.060	0.019, 0.1000	4.08 × 10^-3	4.80 × 10^-1
GCKR	rs780094	2	27594741	C/T	0.620	0.029	0.023, 0.035	0.817	0.022	−0.002, 0.046	7.05 × 10^-2	5.72 × 10^-1
ADCY5	rs11708067	3	124548468	A/G	0.780	0.027	0.021, 0.033	0.843	0.015	−0.010, 0.041	2.31 × 10^-1	3.68 × 10^-1
SLC2A2	rs11920090	3	172200215	T/A	0.870	0.020	0.012, 0.028	0.645	0.005	−0.014, 0.025	5.81 × 10^-1	1.64 × 10^-1
GCK	rs4607517	7	44202193	A/G	0.160	0.062	0.054, 0.070	0.101	0.084	0.050, 0.118	1.42 × 10^-6	2.088 × 10^-1
DGKB-TMEM195	rs2191349	7	15030834	T/G	0.520	0.030	0.024, 0.036	0.593	0.016	−0.003, 0.036	1.02 × 10^-1	1.80 × 10^-1
SLC30A8	rs13266634	8	118253964	C/T	0.680	0.027	0.019, 0.035	0.908	0.004	−0.030, 0.038	8.16 × 10^-1	1.88 × 10^-1
GLIS3	rs7034200	9	4279050	A/C	0.490	0.018	0.012, 0.024	0.630	0.018	−0.001, 0.037	6.15 × 10^-2	1.00 × 10^-0
ADRA2A	rs10885122	10	113032083	G/T	0.870	0.022	0.014, 0.030	0.334	0.006	−0.014, 0.026	5.68 × 10^-1	1.37 × 10^-1
TCF7L2	rs7903146	10	114748339	T/C	0.290	0.023	0.015, 0.031	0.284	0.013	−0.010, 0.035	2.63 × 10^-1	3.93 × 10^-1
MTNR1B	rs10830963	11	92348358	G/C	0.300	0.067	0.061, 0.073	0.073	0.104	0.069, 0.139	8.51 × 10^-9	4.26 × 10^-2
MADD	rs7944584	11	47292896	A/T	0.750	0.021	0.015, 0.027	0.950	0.017	−0.024, 0.059	4.18 × 10^-1	8.50 × 10^-1
FADS1	rs174550	11	61328054	T/C	0.640	0.017	0.011, 0.023	0.916	0.014	−0.019, 0.048	3.99×10^-1	8.62×10^-1
CRY2	rs11605924	11	45829667	A/C	0.490	0.015	0.009, 0.021	0.867	0.016	−0.012, 0.043	2.71×10 ¹	9.44×10 ¹
C2CD4B	rs11071657	15	60221254	A/G	0.630	0.008	0.002, 0.014	0.868	0.006	−0.027, 0.038	7.37×10^-1	9.08×10^-1
FI-associated loci
GCKR	rs780094	2	27594741	C/T	0.620	0.032	0.024, 0.040	0.817	0.025	−0.00, 0.053	7.67 ×10^-2	6.31×10^-1
IGF1	rs35767	12	101399699	G/A	0.850	0.010	−0.002, 0.022	0.570	0.001	−0.022, 0.023	9.49×10^-1	5.02×10^-1

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The loci in this table were all obtained from Dupuis et al (2010) [8]

Heterogeneity test of the β coefficients for EuA populations and AfAs

Effect (trait-raising)/other allele

Chr chromosome; EAF effect allele frequency

Further, in the ±250 kb flanking regions around index SNPs (Table 4), nearly all (17 of 18) had a best SNP nominally significant in AfAs, and four (at GCK, MTNR1B and FADS1 for FG and GCKR for FI) had SNPs that remained significantly associated after adjusting for the effective number of SNPs tested within the regions. At 15 of 18 loci in AfAs, the index SNP and the best SNP were not in substantial LD (all r²< 0.2 in AfAs). At GCK, MTNR1B and CRY2, the index and best SNPs were in strong LD or were the same SNP. Of the best AfA SNPs, five of 18 (at G6PC2, GCKR, GCK, MTNR1B and CRY2) were also at least nominally associated (p<0.05) with FG or FI in those of EuA in the MAGIC data (ESM Table 1).

Table 4. Regional interrogation in AfAs for FG- and FI-related loci previously reported in EuA populations.

Nearest gene(s) FG-associated loci	Index SNP in EuA	Interrogation in AfA within 500 kb region of index SNP

		Best SNP in AfA	Position	LD^a in YRI r² (D′)	LD^b in CEU r² (D′)	Alleles^c	EAF	Effect ([mmol/l] per effect allele)	SE	95% CI	p value	Eff. N^d	Adjusted p value
FG-associated loci
PROX1	rs340874	rs7519597	212261507	0.004 (1.000)	N/A	T/C	0.038	−0.0879	0.0276	−0.142, −0.034	1.42×10^-3	90	1.28×10^-1
G6PC2	rs560887	rs853789	169509734	N/A	0.692 (0.916)	A/G	0.105	−0.0494	0.0157	−0.080, −0.019	1.62×10^-3	105	1.70×10^-1
GCKR	rs780094	rs7586601	27438170	0.169 (0.491)	0.549 (0.956)	A/G	0.239	−0.0303	0.0111	−0.052, −0.009	6.64×10^-3	22	1.46×10^-1
ADCY5	rs11708067	rs10934645	124552510	0.009 (1.000)	0.022 (1.000)	A/G	0.091	0.0573	0.0184	0.021, 0.093	1.86×10^-3	66	1.23 ×10^-1
SLC2A2	rs11920090	rs9827202	172296993	0.005 (0.105)	0.000 (0.118)	T/C	0.443	0.0274	0.0100	0.008, 0.047	5.86×10^-3	65	3.81×10^-1
GCK	rs4607517	rs1799884	44195593	0.469 (1.000)	0.003 (0.196)	T/C	0.177	0.0659	0.0121	0.042, 0.090	5.79×10^-8	34	1.97×10^-6
DGKB-TMEM195	rs2191349	rs17327498	15111235	0.001 (0.184)	0.003 (0.196)	A/G	0.972	0.1132	0.0343	0.046, 0.180	9.66×10^-4	90	8.69×10^-2
SLC30A8	rs13266634	rs2649098	118304453	0.008 (1.000)	0.004 (0.088)	T/C	0.106	0.0477	0.0153	0.018, 0.078	1.83×10^-3	82	1.50×10^-1
GLIS3	rs7034200	rs3895473	4319209	0.003 (0.098)	0.006 (0.158)	A/G	0.800	−0.0417	0.0117	−0.065, −0.019	3.70×10^-4	169	6.25 ×10^-2
ADRA2A	rs10885122	rs7908674	112913361	0.070 (0.426)	0.028 (0.500)	A/G	0.159	0.0345	0.0141	0.007, 0.062	1.42×10^-2	83	1.00×10^-0
TCF7L2	rs7903146	rs7909517	114852926	0.006 (0.255)	0.003 (1.000)	C/G	0.103	0.0606	0.0182	0.025, 0.096	8.42×10^-4	72	6.06 ×10^-2
MTNR1B	rs10830963	rs10830963	92348358	Same SNP	Same SNP	C/G	0.927	−0.1038	0.018	−0.139, −0.069	8.51×10^-9	86	7.32×10^-7
MADD	rs7944584	rs1685404	47200241	N/A	0.258 (1.000)	C/G	0.241	−0.032	0.0109	−0.053, −0.011	3.35×10^-3	27	9.03 ×10^-2
FADS1	rs174550	rs7937198	61525549	0.000 (0.016)	0.003 (0.055)	A/G	0.491	−0.0341	0.0092	−0.052, −0.016	2.22×10^-4	65	1.44×10^-2
CRY2	rs11605924	rs11605924	45829667	Same SNP	Same SNP	A/C	0.867	0.0155	0.0141	−0.012, 0.043	2.71×10^-1	58	1.00×10^-0
C2CD4B	rs11071657	rs893157	60165933	0.019 (0.488)	0.174 (0.527)	T/C	0.584	−0.0264	0.0097	−0.045, −0.007	6.30×10^-3	60	3.78×10^-1
FI-associated loci
GCKR	rs780094	rs7586601	27438170	0.169 (0.491)	0.549 (0.956)	A/G	0.239	−0.0452	0.0131	−0.071, −0.020	5.87×10 ⁴	22	1.29×10 ²
IGF1	rs35767	rs7970320	101508605	0.011 (0.126)	0.001 (0.067)	T/C	0.557	−0.03	0.011	−0.052, −0.008	6.60×10^-3	59	3.89×10^-1

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The loci in this table were all obtained from Dupuis et al (2010) [8]

LD in YRI between the best SNP in AfAs and top SNP in EuA populations

LD in CEU between the best SNP in AfAs and top SNP in EuA populations

Effect (trait-raising)/other allele

Effective number of independent (typed) SNPs interrogated in CARe samples of AfAs

N/A, not available

Associations with HOMA-B (for FG loci) and with HOMA-IR (for FI loci) for both the index SNPs and the best FG or FI SNPs are shown in ESM Tables 2 and 3. Seven SNPs out of 16 EuA FG index SNPs showed nominally significant association with HOMA-B in AfAs, while three best AfA SNPs reached nominal significance. Neither of two EuA FI index SNPs were nominally significant, while the best AfA FI SNPs at both loci were nominally significant (at GCKR and IGF1).

Interrogation of FG or FI-associated type 2 diabetes loci in AfAs

Fourteen of the ∼40 known type 2 diabetes loci have also been nominally associated with FG or FI in individuals of EuA [7, 8]. Of these 14, only TP53INP1 was directionally consistent and nominally associated with FG in AfAs (Table 5). Overall, 8/14 index EuA SNPs had directionally consistent FG associations in AfAs, ten out of 13 best AfA SNPs (one SNP was monomorphic in AfA) showed 95% CIs that overlapped with EuA index SNPs in AfA and eight out of 13 best AfA SNPs did not reach nominal significance level in formal heterogeneity tests comparing those of EuA with AfAs. Note that the loci MTNR1B (p value = 1.38 × 10^-6 for index SNP rs1381753) is again among the loci (THADA, ADAMTS9, HHEX and KCNQ1) with heterogeneous effect (Table 5) and its allelic effect of 0.060 is remarkably larger in the sample of EuA compared with 0.008 in the sample of AfAs. After accounting for ancestral LD by querying SNPs flanking the index SNP, all 14 best SNPs around the 14 index SNPs showed nominally significant associations in AfAs, and four of these best SNPs (at KCNQ1, MTNR1B, ADAMTS9 and CDC123/CAMK1D) remained significantly associated with FG after adjusting for multiple comparisons (Table 6 and ESM Fig. 2). At all 14 loci the best SNP was in low LD with the index SNP (all r² <0.2). The best SNPs at ZBED3, TP53INP1, KCNQ1 and MTNR1B in AfAs were nominally associated with FG in EuA individuals in the MAGIC data (ESM Table 1).

Table 5. Association with FG in AfAs of FG-associated type 2 diabetes risk SNPs previously reported in EuA populations.

Nearest gene(s)	Index SNP^d	Chr.	Position	Alleles^b	EuA FG result for index SNP				AfA FG result for index SNP				Heterogeneity^a

					EAF	Effect ([mmol/l] per effect allele)	95% CI	p value	EAF	Effect ([mmol/l] per effect allele)	95% CI	p value	p value
THADA	rs7578597	2	43586327	C/T	0.9	0.026	0.014, 0.037	2.40×10^-5	0.72	−0.003	−0.023, 0.017	7.81×10^-1	1.29×10^-2
ADAMTS9	rs4607103	3	64686944	T/C	0.76	−0.010	−0.018, −0.001	2.64×10^-2	0.71	0.028	0.008, 0.048	6.85×10^-3	4.18×10^-4
IGF2BP2	rs4402960	3	18699438 1	T/G	0.29	0.011	0.003, 0.018	8.34×10^-3	0.51	−0.003	−0.021, 0.015	7.63×10^-1	1.55×10^-1
ZBED3	rs4457053	5	76460705	G/A	0.26	0.018	0.009, 0.026	9.15×10^-5	N/A	N/A	N/A	N/A	N/A
CDKAL1	rs7754840	6	20769229	C/G	0.31	0.010	0.002, 0.017	1.41×10^-2	0.57	0.008	−0.010, 0.027	3.74×10^-1	8.39×10^-1
TP53INP1	rs896854	8	96029687	T/C	0.48	0.012	0.005, 0.019	8.79×10^-4	0.69	0.022	0.002, 0.042	3.40×10^-2	3.53×10^-1
SLC30A8	rs1326663 4	8	11825396 4	C/T	0.75	0.027	0.018, 0.036	5.46×10^-10	0.91	0.004	−0.030, 0.038	8.16×10^-1	1.88×10^-1
CDKN2A/B	rs1081166 1	9	22124094	T/C	0.79	0.019	0.009, 0.028	9.99×10^-5	0.93	0.026	−0.011, 0.063	1.68×10^-1	7.22×10^-1
CDC123/CAMK1 D	rs1277979 0	10	12368016	A/G	0.18	−0.016	−0.025, −0.006	1.20×10^-3	0.87	−0.014	−0.044, 0.015	3.40×10^-1	8.99×10^-1
HHEX	rs1111875	10	94452862	C/T	0.56	0.009	0.002, 0.016	1.41×10^-2	0.77	−0.017	−0.039, 0.004	1.16×10^-1	2.63×10^-2
KCNQ1	rs231362	11	2648047	G/A	0.52	0.019	0.011, 0.027	5.29×10^-6	0.78	−0.019	−0.044, 0.006	1.46×10^-1	5.21×10^-3
CENTD2^c	rs1552224	11	72110746	A/C	0.88	0.019	0.010, 0.029	1.20×10^-4	0.97	−0.005	−0.065, 0.055	8.73×10^-1	4.45×10^-1
MTNR1B	rs1387153	11	92313476	T/C	0.28	0.060	0.052, 0.069	6.59×10^-45	0.38	0.008	−0.012, 0.028	4.38×10^-1	1.38×10^-6
PRC1	rs8042680	15	89322341	A/C	0.22	0.010	0.002, 0.017	1.30×10^-2	0.84	0.019	−0.007, 0.045	1.50×10^-1	5.08×10^-1

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FG results in EuA are from MAGIC, Dupuis et al (2010) [8], as reported in Voight et al (2010) [7]

Heterogeneity test of the β coefficients for EuA populations and AfAs

Effect/other allele

Also known as ARAP1

Voight et al (2010) [7]; Zeggini et al (2008) [32]; Rung et al (2009) [53]; Altshuler (2000) [54]; Zeggini et al (2007) [33]; Scott et al (2007) [34], Diabetes Genetics Initiative of Broad Institute et al (2007) [55]; Sladek (2007) [36]; Gloyn (2003) [56]; Winckler et al (2007) [37]; Gudmundsson (2007) [58]

Chr., chromosome; N/A, not available

Table 6. Regional interrogation in AfAs for FG-associated type 2 diabetes loci previously reported in EuA populations.

Nearest gene(s)	Index SNP	Interrogation for FG in AfA within 500 kb region of type 2 diabetes index SNP

		Best SNP	Position	LD^a in YRI r² (D′)	LD^b in CEU r² (D′)	Alleles^c	EAF	Effect ([mmol/l] per effect allele)	95% CI	p value	Eff. N^d	Adjusted p value
THADA	rs7578 597	rs6724 325	43529 402	0.066 (0.322)	N/A	T/C	0.1 89	0.036	0.012, 0.059	2.80×103	62	1.74×10^-1
ADAMTS9	rs4607 103	rs4688 504	64659 981	0.020 (0.394)	0.003 (0.080)	T/C	0.319	0.040	0.018, 0.062	3.82×10^-4	125	4.78×10^-2
IGF2BP2	rs4402960	rs7627308	186919528	0.031 (1.000)	N/A	A/G	0.013	0.166	0.052, 0.279	4.19×1^-3	55	2.30×10^-1
ZBED3	rs4457053	rs12517269	76454133	0.001 (0.029)	0.003 (0.080)	A/G	0.883	−0.049	−0.077, −0.021	6.06×10^-4	85	5.15×10^-2
CDKAL1	rs7754840	rs9368255	21008207	0.002 (0.250)	0.019 (1.000)	T/C	0.949	0.057	0.015, 0.099	7.88×10^-3	72	5.67×10^-1
TP53INP1	rs896854	rs509594	96036332	0.069 (1.000)	0.737 (1.000)	C/G	0.795	−0.035	−0.058, −0.012	2.54×10^-3	61	1.55×10^-1
SLC30A8	rs13266634	rs2649098	118304453	0.008 (1.000)	0.004 (0.088)	T/C	0.1 06	0.048	0.018, 0.078	1.83×10^-3	82	1.50×10^-1
CDKN2A/B	rs10811661	rs10965319	22293465	0.092 (1.000)	0.008 (0.216)	A/T	0.8 40	−0.041	−0.068, −0.014	2.86×10^-3	72	2.06×10^-1
CDC123/CA MK1D	rs12779790	rs7074893	12446905	0.000 (1.000)	0.001 (0.068)	T/G	0.9 77	−0.209	−0.312, −0.105	8.29×10^-5	89	7.38×10^-3
HHEX	rs1111875	rs11187186	94576008	0.000 (0.017)	0.000 (0.017)	T/C	0.457	−0.029	−0.048, −0.010	2.24×10^-3	42	9.41×10^-2
KCNQ1	rs231362	rs2011766	2490838	0.000 (0.033)	0.001 (0.085)	T/C	0.3 32	−0.044	−0.068, −0.021	2.02×10^-4	91	1.84×10^-2
CENTD2^e	rs1552224	rs6592481	72067275	N/A	0.001 (0.273)	T/C	0.220	0.038	0.012, 0.064	4.79×10^-3	51	2.44×10^-1
MTNR1B	rs1387153	rs10830963	92348358	0.036 (0.710)	0.705 (0.874)	C/G	0.927	−0.104	−0.139, −0.069	8.51×10^-9	87	7.40×10^-7
PRC1	rs8042680	rs10083635	89195519	0.006 (1.000)	N/A	A/G	0.145	0.060	0.025, 0.094	6.53×10^-4	98	6.40×10^-2

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Index SNP data are from Voight et al (2010) [7]; Zeggini et al (2008) [32]; Rung et al (2009) [53]; Altshuler (2000) [54]; Zeggini et al (2007) [33]; Scott et al (2007) [34]; Diabetes Genetics Initiative of Broad Institute et al (2007) [55]; Sladek (2007) [36]; Gloyn (2003) [56]; Winckler et al (2007) [57]; Gudmundsson (2007) [58]

LD in YRI between the best SNP in AfAs and top SNP in EuA populations

LD in CEU between the best SNP in AfAs and top SNP in EuA populations

Effect/other allele

Effective number of independent (typed) SNPs interrogated in CARe samples of AfA

Also known as ARAP1

N/A, not available

Discussion

We found that among 18 index SNPs robustly associated with glycaemic quantitative traits in the MAGIC EuA populations, just three SNPs (at GCK, MTNR1B and G6PC2 for FG) were directly transferrable to the ∼6,000 AfA individuals from five separate cohorts in CARe, but all 18 published 95% CIs around association-point estimates in MAGIC overlapped with the CIs based on associations in AfAs. However, after accounting for ancestral differences in LD by searching more broadly in regions flanking the EuA index SNP, 17 of 18 loci were nominally associated with FG or FI in AfAs, and SNPs at four of these loci (GCK, MTNR1B and FADS1 for FG and GCKR for FI) remained significantly associated with FG or FI in AfAs after accounting for multiple comparisons. For FG-associated type 2 diabetes SNPs discovered in EuA populations, only one (TP53INP1) was directly transferable to AfAs, but ten of 13 published 95% CIs overlapped with the CIs based on associations in AfA. These overlapping intervals indicate that the trait-raising allelic effects for glycaemic loci are similar in individuals of EuA and AfAs. Four FG-associated type 2 diabetes loci (ADAMTS9, CDC123/CAMK1D, KCNQ1 and MTNR1B) had a best AfA SNP significantly associated with FG in AfAs after adjusting for multiple comparisons. As expected, allele frequencies and regional LD varied widely: there was evidence of modest population differentiation for six index SNPs (including FADS1), but none showed evidence of recent positive selection. These results provide evidence that some genetic loci associated with glycaemic regulation are reproducibly shared across human populations, with a suggestion of potential transferability for most loci, at least for individuals of European or African ancestry. The data also provide evidence for allelic heterogeneity at many loci and evidence for shared haplotypes with proximity of an index SNP with a functional variant at several loci.

These results illustrate the value of examining AfA cohorts for fine-mapping. Where there is strong LD between the index SNP and the best SNP, association with FG in an ancestral group with reduced LD increases confidence that the index signal is on the same haplotype or is otherwise close to a functional mutation (GCK, MTNR1B and CRY2). For instance, at MTNR1B, the FG–type 2 diabetes signal is the same for both those of EuA and AfAs (rs10830963). Where there is weak LD between the index SNP and the best SNP (all other loci in AfAs), there are two alternative explanations: [1] these SNPs represent independent signals where the index and best SNPs were significant in only one population (population-specific allelic heterogeneity); or [2] they share LD with a tagged untyped functional SNP in the region where the differential LD pattern between those of EuA and AfAs can focus fine-mapping of the association signal. For instance, at G6PC2 in EuA the FG index SNP (rs560887) and best SNP (rs853789) are in strong LD with a known functional SNP (rs13431652; r² with rs560887 in CEU = 0.923, r² with rs853789 in CEU = 0.595) [38], but AfA rs853789 is not correlated with rs13431652 (r² in YRI = 0.004). KCNQ1 also demonstrates multiple independent signals identified in different ancestry groups [7, 39, 40]. Variation in LD structure across these ancestral groups has focused regions of interest and identified new signals that may aid future fine-mapping to identify functional variants involved in FG and FI regulation.

Just one paper has investigated the transferability of EuA diabetes-related quantitative trait loci in AfAs. Ramos et al examined the index and correlated SNPs (r² ≥ 0.3 in CEU) for 16 fasting glucose loci reported by MAGIC in 927 AfAs [19], demonstrating associations of index and/or correlated SNPs at TMEM195 (also known as AGMO), SLC30A8, TCF7L2, G6PC2, GCKR and MTNR1B. This is consistent with our index SNP association results at G6PC2 and MTNR1B and regional association at GCKR. In addition, the direction and magnitude of effect of the T allele in rs2191349 at TMEM195 were comparable (β = 0.014 [Ramos] vs 0.016 [this study]), despite not reaching significance in this study. Taken together with the significant association of AfA best SNPs at GCKR, GCK and MTNR1B with FG or FI in EuA populations in MAGIC, the loci GCKR, GCK and MTNR1B appear likely to share the same causal variant(s) in populations with EuA and AfAs. Differences in LD such as those found at GCK and GCKR between those with EuA and AfAs also facilitate the fine-mapping of causal variants in these loci. The associations of independent SNPs at G6PC2 and FADS1 in EuA populations and AfAs probably point to distinct signals requiring further study.

Our data add to the list of glycaemic loci confirmed and informatively fine-mapped by cross-ancestry analyses [41-43]. For instance, at KCNQ1, studies in South and East Asian populations identified the type-2-diabetes-associated SNPs rs2237892, rs2237895 and rs2237897 [39, 40], weakly correlated with the signal seen in EuA populations (rs231362) [7]. Here, we add an FG-associated signal in AfAs (rs2011766), bringing to five the total number of apparently independent signals at KCNQ1 associated with glycaemia in humans. SNP rs2011766 is intronic and is in very weak LD with the other SNPs in the 1000 Genomes Pilot 1 reference panel: rs231362 vs rs2011766, r² = 0.06; rs2011766 vs rs2237895 r² = 0.03; and rs2011766 vs rs2237892 r² = 0.02. However, rs2011766 does not appear to be a type-2-diabetes-risk locus, with an odds ratio for type 2 diabetes in the DIAGRAM discovery meta-analysis of 1.04 (p=0.19) [7]. Multi-ethnic allelic heterogeneity in association with FG or type 2 diabetes has also been reported for the C2CD4A/B locus [44]. Other loci at which cross-ancestry fine-mapping has confirmed the index SNP to be the same in EuA populations and AfAs are TCF7L2 [10, 13, 45, 46], PPARG, IGF2BP2, JAZF1 [12] and FTO [47].

Type 2 diabetes disproportionately affects AfAs. This differential risk may be due partly to genetic factors [5]. Most AfAs have differential mixed ancestry from Western Africa and Europe [48]. AfA genetic architectures are substantially different and generally have lower LD compared with other US populations [5], which is theoretically able to assist fine-mapping of causal variants. Our observation of large differences in risk-allele frequency and high F_st between AfAs and EuA populations at some SNPs is consistent with studies of other complex-disease-associated SNPs [4, 49]. Differences in risk-allele frequencies or in ability to increase FG (ancestral vs derived allele) did not seem to have strong impact on the association as supported by many of the loci showing transferability with similar effect sizes in AfAs [16]; of 18 glycaemic loci, only SNPs at FADS1 had evidence of population differentiation and significant association with FG at weakly correlated EuA and AfA signals. A limitation of our study is that lower risk-allele frequencies at some loci may be due to insufficient study power. In addition, lack of transferability may arise from the confounding effects of social and environmental risk exposures that vary across populations and that have not been considered here [50-52].

Conclusions

Cross-ancestry studies can show new population-specific risk variants and confirm shared risk variants. Allele frequencies and LD variation influence power and the ability to resolve the signal to a smaller region containing possible functional variants. In the AfA CARe cohort of ∼6,000 person, there was substantial allele frequency variation but modest EuA index SNP transferability. However, most glycaemia-associated loci showed at least nominal evidence of similar association in AfA and EuA groups after accounting for LD differences. Four FG loci (G6PC2, GCK, MTNR1B and FADS1) and three type 2 diabetes-FG loci (KCNQ1, ADAMTS9 and CDC123/CAMK1D) were convincingly transferable from EuA populations to AfAs. Cross-ancestry analyses that account for and leverage varying LD across ancestral groups can identify signals that may aid fine-mapping. In the near future one can expect that new or confirmatory variants associated with type 2 diabetes and diabetes-related quantitative traits are likely to be revealed by more extensive sequencing and genome-wide analysis ongoing in other ancestral population groups, further illuminating the genetic architecture of type 2 diabetes risk and glycaemic regulation in all humans.

Supplementary Material

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Acknowledgments

The authors wish to acknowledge the support of the NHLBI and the contributions of the research institutions, study investigators and field staff involved, and the study participants of ARIC, CARDIA, CFS, JHS and MESA in creating the CARe for biomedical research (CARe; http://public.nhlbi.nih.gov/GeneticsGenomics/home/care.aspx). The following five parent studies have contributed parent study data, ancillary study data and DNA samples through the Massachusetts Institute of Technology - Broad Institute (N01-HC-65226) to create this genotype/phenotype database for wide dissemination to the biomedical research community: ARIC; CFS; JHS; CARDIA; and MESA.

Funding: This work was supported by NIDDK R01 2 DK078616 and NIDDK K24 DK080140 to J. B. Meigs, a Doris Duke Charitable Foundation Clinical Scientist Development Award to J. C. Florez, RO1 DK066358 to D. W. Bowden, 5RC1HL099911-02 5UC2HL103010-02 and the NHLBI MESA contract N01HC95165-21-0-1 to S. S. Rich.

ARIC is carried out as a collaborative study supported by NHBLI contracts N01-HC-55015, N01-HC-55016, N01-HC-55018, N01-HC-55019, N01-HC-55020, N01-HC-55021, and N01-HC-55022. CFS: Case Western Reserve University (NIH HL 46380, M01RR00080).

JHS is carried out as a collaborative study supported by Jackson State University (N01-HC-95170), University of Mississippi (N01-HC-95171), Tougaloo College (N01-HC-95172);

MESA is conducted and supported by the NHLBI in collaboration with MESA investigators. Support is provided by grants and contracts N01-HD-95159 through N01-HC-95169, RR-024156, and R01-HL-071205.

CARDIA is carried out as a collaborative study supported by University of Alabama at Birmingham (N01-HC-48047), University of Minnesota (N01-HC-48048), Northwestern University (N01-HC-48049), Kaiser Foundation Research Institute (N01-HC-48050), University of Alabama at Birmingham (N01-HC-95095), Tufts-New England Medical Center (N01-HC-45204), Wake Forest University (N01-HC-45205), Harbor-UCLA Research and Education Institute (N01-HC-05187), University of California Irvine (N01-HC-45134, N01-HC-95100).

Abbreviations

AfA: African-American
ARIC: Atherosclerosis Risk in Communities
CARDIA: Coronary Artery Risk Development in young Adults
CARe: Candidate Gene Association Resource
CEU: HapMap 2 reference panel of Utah residents of northern and western European ancestry
CFS: Cleveland Family Study
CVD: Cardiovascular disease
DIAGRAM: The Diabetes Genetics Replication And Meta-analysis Consortium
EuA: European ancestry
FG: Fasting glucose
FI: Fasting insulin
F_st: Fixation index
HOMA-B: HOMA-beta cell function
HOMA-IR: HOMA- insulin resistance
iHS: Integrated haplotype score
JHS: Jackson Heart Study
LD: Linkage disequilibrium
MAGIC: The Meta-Analyses of Glucose and Insulin-related traits Consortium
MESA: Multi-Ethnic Study of Atherosclerosis
MSA: Metropolitan statistical area
NHLBI: National Heart, Lung, and Blood Institute
SNP: Single-nucleotide polymorphism
varLD: A program for quantifying variation in linkage disequilibrium patterns between populations
YRI: HapMap 2 reference panel of residents of Yoruba in Ibadan, Nigeria

Footnotes

Electronic supplementary material: The online version of this article (doi:10.1007/s00125-012-2656-4) contains peer-reviewed but unedited supplementary material, which is available to authorised users.

Contribution statement: The experiments were conceived and designed by SR, WHK, DS, JCF, JIR, JGW, DWB and JBM. C-TL, DR, JD, MCYN, SRP, WHK and KLL analysed the data. The paper was written by: C-TL, MCYN, JCF, JD, and JBM. SR, WHK, DS, JCF, JIR, JGW, DWB and JBM recruited individuals. Analysis and interpretation of data and critical review of manuscript were performed by C-TL, MCYN, DR, AA, SJB, EB, IB, BC, YDIC, LD, MF, MOG, SFAG, XG, TH, EK, JRK, YL, KLL, KM, JAN, JSP, SRP, ER, LR-T, SSR, CNR, DS, DS, MS, JMZ, SR, WHK, DS, JCF, JIR, JD, JGW, DWB and JBM.

JBM and C-TL are the guarantors of this work, had full access to all the data and take full responsibility for the integrity of data and the accuracy of data analysis. All authors have approved the final version of manuscript.

Duality of interest: The authors declare that there is no duality of interest associated with this manuscript.

Contributor Information

C.-T. Liu, Department of Biostatistics, Boston University School of Public Health, Boston, MA, USA

M. C. Y. Ng, Center for Genomics and Personalized Medicine Research, Center for Diabetes Research, Wake Forest University School of Medicine, Winston-Salem, NC, USA

D. Rybin, Boston University Data Coordinating Center, Boston, MA, USA

A. Adeyemo, National Human Genome Research Institute, Bethesda, MD, USA

S. J. Bielinski, Mayo Clinic, Rochester, MN, USA

E. Boerwinkle, University of Texas Health Science Center at Houston, Houston, TX, USA

I. Borecki, Washington University, St Louis, MO, USA

B. Cade, Brigham and Women's Hospital, Boston, MA, USA

Y. D. I. Chen, Cedars Sinai Medical Center, Los Angeles, CA, USA

L. Djousse, Email: jmeigs@partners.org, Brigham and Women's Hospital, Boston, MA, USA; Department of Medicine, Harvard Medical School, Boston, MA, USA; Boston VA Healthcare System, Boston, MA, USA;.

M. Fornage, University of Texas Health Science Center at Houston, Houston, TX, USA

M. O. Goodarzi, Cedars Sinai Medical Center, Los Angeles, CA, USA

S. F. A. Grant, Children's Hospital of Philadelphia, Philadelphia, PA, USA

X. Guo, Cedars Sinai Medical Center, Los Angeles, CA, USA

T. Harris, National Institute on Aging, Bethesda, MD, USA

E. Kabagambe, University of Alabama, Birmingham, AL, USA

J. R. Kizer, Cornell University, New York, NY, USA

Y. Liu, Center for Genomics and Personalized Medicine Research, Center for Diabetes Research, Wake Forest University School of Medicine, Winston-Salem, NC, USA; Department of Epidemiology and Prevention, Wake Forest University, Winston-Salem, North Carolina, USA

K. L. Lunetta, Department of Biostatistics, Boston University School of Public Health, Boston, MA, USA; National Heart, Lung, and Blood Institute' Framingham Heart Study, Framingham, MA, USA

K. Mukamal, Department of Medicine, Harvard Medical School, Boston, MA, USA

J. A. Nettleton, University of Texas Health Science Center at Houston, Houston, TX, USA

J. S. Pankow, University of Minnesota, Minneapolis, MN, USA

S. R. Patel, Brigham and Women's Hospital, Boston, MA, USA

E. Ramos, National Human Genome Research Institute, Bethesda, MD, USA

L. Rasmussen-Torvik, Northwestern University, Chicago, IL, USA

S. S. Rich, University of Virginia, Charlottesville, VA, USA

C. N. Rotimi, National Human Genome Research Institute, Bethesda, MD, USA

D. Sarpong, Jackson State University, Jackson, MS, USA

D. Shriner, National Human Genome Research Institute, Bethesda, MD, USA

M. Sims, University of Mississippi Medical Center, Jackson, MS, USA

J. M. Zmuda, University of Pittsburgh, Graduate School of Public Health, Pittsburgh, PA, USA

S. Redline, Brigham and Women's Hospital, Boston, MA, USA

W. H. Kao, Johns Hopkins University, Baltimore, MD, USA

D. Siscovick, University of Washington, Seattle, WA, USA

J. C. Florez, Department of Medicine, Harvard Medical School, Boston, MA, USA; Diabetes Unit and Center for Human Genetic Research, Massachusetts General Hospital, Boston, MA, USA; Program in Medical and Population Genetics, Broad Institute, Cambridge, MA, USA

J. I. Rotter, Cedars Sinai Medical Center, Los Angeles, CA, USA

J. Dupuis, Department of Biostatistics, Boston University School of Public Health, Boston, MA, USA; National Heart, Lung, and Blood Institute's Framingham Heart Study, Framingham, MA, USA

J. G. Wilson, University of Mississippi Medical Center, Jackson, MS, USA

D. W. Bowden, Center for Genomics and Personalized Medicine Research, Center for Diabetes Research, Wake Forest University School of Medicine, Winston-Salem, NC, USA; Departments of Biochemistry and Internal Medicine, Wake Forest University School of Medicine, Winston-Salem, NC, USA

J. B. Meigs, Department of Medicine, Harvard Medical School, Boston, MA, USA; General Medicine Division, Massachusetts General Hospital, 50 Staniford Street, 9th Flr, Boston, MA, USA

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Associated Data

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Supplementary Materials

125_2012_2656_MOESM1_ESM

NIHMS400966-supplement-1.pdf^{(12.1KB, pdf)}

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NIHMS400966-supplement-2.pdf^{(794.8KB, pdf)}

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PERMALINK

Transferability and fine-mapping of glucose and insulin quantitative trait loci across populations: CARe, the Candidate Gene Association Resource

C-T Liu

M C Y Ng

D Rybin

A Adeyemo

S J Bielinski

E Boerwinkle

I Borecki

B Cade

Y D I Chen

L Djousse

M Fornage

M O Goodarzi

S F A Grant

X Guo

T Harris

E Kabagambe

J R Kizer

Y Liu

K L Lunetta

K Mukamal

J A Nettleton

J S Pankow

S R Patel

E Ramos

L Rasmussen-Torvik

S S Rich

C N Rotimi

D Sarpong

D Shriner

M Sims

J M Zmuda

S Redline

W H Kao

D Siscovick

J C Florez

J I Rotter

J Dupuis

J G Wilson

D W Bowden

J B Meigs

Abstract

Aims/hypothesis

Methods

Results

Conclusions/interpretation

Methods

Candidate Gene Association Resource study samples

Atherosclerosis Risk in Communities

Coronary Artery Risk Development in young Adults

Cleveland Family Study

Jackson Heart Study

Multi-Ethnic Study of Atherosclerosis

Genotyping, imputation and principal components analysis

Allele frequency differences and natural selection

Statistical analysis to generate SNP-trait-association results

Fig. 1.

Interrogation of transferability

Results

Table 1. Characteristics of five CARe cohorts.

Allele frequency differences and natural selection

Table 2. Allele frequency differences, Fsts and iHSs in AfA and EuA populations.

Interrogation of EuA FG- and FI-associated loci in AfAs

Table 3. Transferability to AfAs of FG- and FI-related loci previously reported in EuA populations.

Table 4. Regional interrogation in AfAs for FG- and FI-related loci previously reported in EuA populations.

Interrogation of FG or FI-associated type 2 diabetes loci in AfAs

Table 5. Association with FG in AfAs of FG-associated type 2 diabetes risk SNPs previously reported in EuA populations.

Table 6. Regional interrogation in AfAs for FG-associated type 2 diabetes loci previously reported in EuA populations.

Discussion

Conclusions

Supplementary Material

Acknowledgments

Abbreviations

Footnotes

Contributor Information

References

Associated Data

Supplementary Materials

ACTIONS

Table 2. Allele frequency differences, F_sts and iHSs in AfA and EuA populations.