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. 1996 Oct 15;93(21):11288–11294. doi: 10.1073/pnas.93.21.11288

Site-specific integration by adeno-associated virus.

R M Linden 1, P Ward 1, C Giraud 1, E Winocour 1, K I Berns 1
PMCID: PMC38050  PMID: 8876128

Abstract

Adeno-associated virus (AAV) has attracted considerable interest as a potential vector for gene delivery. Wild-type virus is notable for the lack of association with any human disease and the ability to stably integrate its genome in a site-specific manner in a locus on human chromosome 19 (AAVS1). Use of a functional model system for AAV DNA integration into AAVS1 has allowed us to conclude that the recombination event is directed by cellular DNA sequences. Recombinant junctions isolated from our integration assay were analyzed and showed characteristics similar to those found in latently infected cell lines. The minimal DNA signals within AAVS1 required for targeted integration were identified and shown to contain functional motifs of the viral origin of replication. A replication mediated model of AAV DNA integration is proposed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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