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. 1972 Jun;23(6):1082–1089. doi: 10.1128/am.23.6.1082-1089.1972

Microbial Utilization of Crude Oil

A Jobson 1, F D Cook 1, D W S Westlake 1
PMCID: PMC380511  PMID: 4557559

Abstract

The utilization of two crude oil samples of different quality at 4 and 30 C has been studied by using pure and mixed bacterial cultures obtained by enrichment procedures. Growth, emulsification, and utilization occurred readily at both temperatures. The crude oil residue is increased in specific gravity and readily sediments out of solution. A comparison of the chemical analysis of the oils by liquid and gas-liquid chromatographic procedures before and after growth showed that the n-saturate fraction had been preferentially used. Some utilization of the aromatic fraction also occurred. Enrichments obtained with a high-quality crude oil were not as effective in utilizing a lower quality crude oil as sole carbon source as a population enriched on the low-quality crude oil.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. FOSTER J. W. Hydrocarbons as substrates for microorganisms. Antonie Van Leeuwenhoek. 1962;28:241–274. doi: 10.1007/BF02538739. [DOI] [PubMed] [Google Scholar]
  2. HANSEN R. W., KALLIO R. E. Inability of nitrate to serve as a terminal oxidant for hydrocarbons. Science. 1957 Jun 14;125(3259):1198–1199. doi: 10.1126/science.125.3259.1198. [DOI] [PubMed] [Google Scholar]
  3. McKenna E. J., Kallio R. E. Microbial metabolism of the isoprenoid alkane pristane. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1552–1554. doi: 10.1073/pnas.68.7.1552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Raymond R. L., Jamison V. W., Hudson J. O. Hydrocarbon cooxidation in microbial systems. Lipids. 1971 Jul;6(7):453–457. doi: 10.1007/BF02531228. [DOI] [PubMed] [Google Scholar]
  5. Stone R. W., Fenske M. R., White A. G. Bacteria Attacking Petroleum and Oil Fractions. J Bacteriol. 1942 Aug;44(2):169–178. doi: 10.1128/jb.44.2.169-178.1942. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. van der Linden A. C., Thijsse G. J. The mechanisms of microbial oxidations of petroleum hydrocarbons. Adv Enzymol Relat Areas Mol Biol. 1965;27:469–546. doi: 10.1002/9780470122723.ch10. [DOI] [PubMed] [Google Scholar]

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