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. 1972 Jun;23(6):1097–1100. doi: 10.1128/am.23.6.1097-1100.1972

Agar Block Technique for Identification of Mycoplasmas by Use of Fluorescent Antibody1

E J Baas a,2, D E Jasper a
PMCID: PMC380513  PMID: 4114436

Abstract

A procedure for staining mycoplasmata colonies directly on agar blocks for examination by fluorescent microscopy is described. Areas of the agar surface appropriate for staining were demarcated by use of Lucite cylinders. Direct fluorescent-antibody staining was superior to indirect staining. The technique was very useful for determining whether cultures were mixed and for identification of mycoplasmas in either pure or mixed cultures.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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