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. 1972 Jul;24(1):22–25. doi: 10.1128/am.24.1.22-25.1972

Indirect Hemagglutination Test for Chlamydial Antibodies

Vester J Lewis 1, W Lanier Thacker 1, Helen M Engelman 1
PMCID: PMC380539  PMID: 4626906

Abstract

An indirect hemagglutination (IHA) test is described for chlamydial antibodies in psittacosis diagnostic sera; for this test tanned sheep erythrocytes sensitized with a deoxycholate extract of Chlamydia psittaci grown in Vero cell monolayers were used. Adaptation of the IHA test to the Microtiter system decreased sensitivity; nevertheless, the Microtiter-IHA test was more sensitive than the complement fixation test. Lymphogranuloma venereum antibodies also were detected by using antigen extracted from C. psittaci.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BENEDICT A. A., O'BRIEN E. A passive hemagglutination reaction for psittacosis. J Immunol. 1958 Feb;80(2):94–99. [PubMed] [Google Scholar]
  2. BOYDEN S. V. The adsorption of proteins on erythrocytes treated with tannic acid and subsequent hemagglutination by antiprotein sera. J Exp Med. 1951 Feb;93(2):107–120. doi: 10.1084/jem.93.2.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dhir S. P., Kenny G. E., Grayston J. T. Characterization of the group antigen of Chlamydia trachomatis. Infect Immun. 1971 Dec;4(6):725–730. doi: 10.1128/iai.4.6.725-730.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  5. FRASER C. E., BERMAN D. T. TYPE-SPECIFIC ANTIGENS IN THE PSITTACOSIS-LYMPHOGRANULOMA VENEREUM GROUP OF ORGANISMS. J Bacteriol. 1965 Apr;89:943–948. doi: 10.1128/jb.89.4.943-948.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. GIMENEZ D. F. STAINING RICKETTSIAE IN YOLK-SAC CULTURES. Stain Technol. 1964 May;39:135–140. doi: 10.3109/10520296409061219. [DOI] [PubMed] [Google Scholar]
  7. JENKIN H. M., ROSS M. R., MOULDER J. W. Species-specific antigens from the cell walls of the agents of meningopneumonitis and feline pneumonitis. J Immunol. 1961 Feb;86:123–127. [PubMed] [Google Scholar]
  8. STAVITSKY A. B. Micromethods for the study of proteins and antibodies. I. Procedure and general applications of hemagglutination and hemagglutination-inhibition reactions with tannic acid and protein-treated red blood cells. J Immunol. 1954 May;72(5):360–367. [PubMed] [Google Scholar]
  9. Turner W., Gordon F. B. Indirect hemagglutination with the trachoma agent and related microorganisms. J Bacteriol. 1964 May;87(5):1251–1252. doi: 10.1128/jb.87.5.1251-1252.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Vedros N. A. Species-specific antigens from trachoma and inclusion-conjuctivitis (chlamydial) agents. J Immunol. 1967 Dec;99(6):1183–1186. [PubMed] [Google Scholar]

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