Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1996 Oct 15;93(21):11378–11381. doi: 10.1073/pnas.93.21.11378

Early events in poliovirus infection: virus-receptor interactions.

V R Racaniello 1
PMCID: PMC38065  PMID: 8876143

Abstract

The interaction of poliovirus with its cell receptor initiates conformational changes that lead to uncoating of the viral RNA. Three types of genetic analyses have been used to study the poliovirus-receptor interaction: (i) mutagenesis of the poliovirus receptor (PVR), (ii) selection of viral mutants resistant to neutralization with soluble PVR, and (iii) selection of viral variants adapted to use mutant PVRs. The results of these studies show that a small portion of the first immunoglobulin-like domain of PVR contacts viral residues within a deep depression on the surface of the capsid that encircles the fivefold axis of symmetry. Viral capsid residues that influence the interaction with PVR are also found in locations such as the capsid interior that cannot directly contact PVR. These mutations might influence the ability of the capsid to undergo receptor-mediated conformational transitions that are necessary for high-affinity interactions with PVR.

Full text

PDF
11379

Images in this article

11379Fig. 1
on p.11379
11380Fig. 2
on p.11380

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernhardt G., Bibb J. A., Bradley J., Wimmer E. Molecular characterization of the cellular receptor for poliovirus. Virology. 1994 Feb 15;199(1):105–113. doi: 10.1006/viro.1994.1102. [DOI] [PubMed] [Google Scholar]
  2. Bibb J. A., Bernhardt G., Wimmer E. The human poliovirus receptor alpha is a serine phosphoprotein. J Virol. 1994 Sep;68(9):6111–6115. doi: 10.1128/jvi.68.9.6111-6115.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Colston E. M., Racaniello V. R. Poliovirus variants selected on mutant receptor-expressing cells identify capsid residues that expand receptor recognition. J Virol. 1995 Aug;69(8):4823–4829. doi: 10.1128/jvi.69.8.4823-4829.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Colston E., Racaniello V. R. Soluble receptor-resistant poliovirus mutants identify surface and internal capsid residues that control interaction with the cell receptor. EMBO J. 1994 Dec 15;13(24):5855–5862. doi: 10.1002/j.1460-2075.1994.tb06930.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Eberlé F., Dubreuil P., Mattei M. G., Devilard E., Lopez M. The human PRR2 gene, related to the human poliovirus receptor gene (PVR), is the true homolog of the murine MPH gene. Gene. 1995 Jul 4;159(2):267–272. doi: 10.1016/0378-1119(95)00180-e. [DOI] [PubMed] [Google Scholar]
  6. Filman D. J., Syed R., Chow M., Macadam A. J., Minor P. D., Hogle J. M. Structural factors that control conformational transitions and serotype specificity in type 3 poliovirus. EMBO J. 1989 May;8(5):1567–1579. doi: 10.1002/j.1460-2075.1989.tb03541.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fricks C. E., Hogle J. M. Cell-induced conformational change in poliovirus: externalization of the amino terminus of VP1 is responsible for liposome binding. J Virol. 1990 May;64(5):1934–1945. doi: 10.1128/jvi.64.5.1934-1945.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Greber U. F., Willetts M., Webster P., Helenius A. Stepwise dismantling of adenovirus 2 during entry into cells. Cell. 1993 Nov 5;75(3):477–486. doi: 10.1016/0092-8674(93)90382-z. [DOI] [PubMed] [Google Scholar]
  9. Gómez Yafal A., Kaplan G., Racaniello V. R., Hogle J. M. Characterization of poliovirus conformational alteration mediated by soluble cell receptors. Virology. 1993 Nov;197(1):501–505. doi: 10.1006/viro.1993.1621. [DOI] [PubMed] [Google Scholar]
  10. Harber J., Bernhardt G., Lu H. H., Sgro J. Y., Wimmer E. Canyon rim residues, including antigenic determinants, modulate serotype-specific binding of polioviruses to mutants of the poliovirus receptor. Virology. 1995 Dec 20;214(2):559–570. doi: 10.1006/viro.1995.0067. [DOI] [PubMed] [Google Scholar]
  11. Hogle J. M., Chow M., Filman D. J. Three-dimensional structure of poliovirus at 2.9 A resolution. Science. 1985 Sep 27;229(4720):1358–1365. doi: 10.1126/science.2994218. [DOI] [PubMed] [Google Scholar]
  12. JOKLIK W. K., DARNELL J. E., Jr The adsorption and early fate of purified poliovirus in HeLa cells. Virology. 1961 Apr;13:439–447. doi: 10.1016/0042-6822(61)90275-6. [DOI] [PubMed] [Google Scholar]
  13. Kaplan G., Freistadt M. S., Racaniello V. R. Neutralization of poliovirus by cell receptors expressed in insect cells. J Virol. 1990 Oct;64(10):4697–4702. doi: 10.1128/jvi.64.10.4697-4702.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kaplan G., Peters D., Racaniello V. R. Poliovirus mutants resistant to neutralization with soluble cell receptors. Science. 1990 Dec 14;250(4987):1596–1599. doi: 10.1126/science.2177226. [DOI] [PubMed] [Google Scholar]
  15. Lopez M., Eberlé F., Mattei M. G., Gabert J., Birg F., Bardin F., Maroc C., Dubreuil P. Complementary DNA characterization and chromosomal localization of a human gene related to the poliovirus receptor-encoding gene. Gene. 1995 Apr 3;155(2):261–265. doi: 10.1016/0378-1119(94)00842-g. [DOI] [PubMed] [Google Scholar]
  16. Martin A., Wychowski C., Couderc T., Crainic R., Hogle J., Girard M. Engineering a poliovirus type 2 antigenic site on a type 1 capsid results in a chimaeric virus which is neurovirulent for mice. EMBO J. 1988 Sep;7(9):2839–2847. doi: 10.1002/j.1460-2075.1988.tb03140.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Mendelsohn C. L., Wimmer E., Racaniello V. R. Cellular receptor for poliovirus: molecular cloning, nucleotide sequence, and expression of a new member of the immunoglobulin superfamily. Cell. 1989 Mar 10;56(5):855–865. doi: 10.1016/0092-8674(89)90690-9. [DOI] [PubMed] [Google Scholar]
  18. Morrison M. E., He Y. J., Wien M. W., Hogle J. M., Racaniello V. R. Homolog-scanning mutagenesis reveals poliovirus receptor residues important for virus binding and replication. J Virol. 1994 Apr;68(4):2578–2588. doi: 10.1128/jvi.68.4.2578-2588.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Morrison M. E., Racaniello V. R. Molecular cloning and expression of a murine homolog of the human poliovirus receptor gene. J Virol. 1992 May;66(5):2807–2813. doi: 10.1128/jvi.66.5.2807-2813.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Murray M. G., Bradley J., Yang X. F., Wimmer E., Moss E. G., Racaniello V. R. Poliovirus host range is determined by a short amino acid sequence in neutralization antigenic site I. Science. 1988 Jul 8;241(4862):213–215. doi: 10.1126/science.2838906. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Olson N. H., Kolatkar P. R., Oliveira M. A., Cheng R. H., Greve J. M., McClelland A., Baker T. S., Rossmann M. G. Structure of a human rhinovirus complexed with its receptor molecule. Proc Natl Acad Sci U S A. 1993 Jan 15;90(2):507–511. doi: 10.1073/pnas.90.2.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H. J., Johnson J. E., Kamer G., Luo M., Mosser A. G. Structure of a human common cold virus and functional relationship to other picornaviruses. Nature. 1985 Sep 12;317(6033):145–153. doi: 10.1038/317145a0. [DOI] [PubMed] [Google Scholar]
  23. Ryu S. E., Kwong P. D., Truneh A., Porter T. G., Arthos J., Rosenberg M., Dai X. P., Xuong N. H., Axel R., Sweet R. W. Crystal structure of an HIV-binding recombinant fragment of human CD4. Nature. 1990 Nov 29;348(6300):419–426. doi: 10.1038/348419a0. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Shepley M. P., Racaniello V. R. A monoclonal antibody that blocks poliovirus attachment recognizes the lymphocyte homing receptor CD44. J Virol. 1994 Mar;68(3):1301–1308. doi: 10.1128/jvi.68.3.1301-1308.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Shepley M. P., Sherry B., Weiner H. L. Monoclonal antibody identification of a 100-kDa membrane protein in HeLa cells and human spinal cord involved in poliovirus attachment. Proc Natl Acad Sci U S A. 1988 Oct;85(20):7743–7747. doi: 10.1073/pnas.85.20.7743. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. White J. M. Viral and cellular membrane fusion proteins. Annu Rev Physiol. 1990;52:675–697. doi: 10.1146/annurev.ph.52.030190.003331. [DOI] [PubMed] [Google Scholar]
  27. Zibert A., Selinka H. C., Elroy-Stein O., Wimmer E. The soluble form of two N-terminal domains of the poliovirus receptor is sufficient for blocking viral infection. Virus Res. 1992 Sep 1;25(1-2):51–61. doi: 10.1016/0168-1702(92)90099-u. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES