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. 1972 Nov;24(5):742–749. doi: 10.1128/am.24.5.742-749.1972

Simplified Radioimmunoassay for Diagnostic Serology

Harriet D Hutchinson 1, Donald W Ziegler 1
PMCID: PMC380656  PMID: 4344958

Abstract

A simplified, indirect radioimmunoassay is described for Escherichia coli, vaccinia virus, and herpesvirus. The antigens were affixed to glass cover slips; thus both the primary and secondary reactions take place on the cover slips, and the unbound antiserum is easily separated from the bound antiserum by rinsing. Rabbit or human immune sera were reacted with the antigens, and the primary immune complex was quantitated by a secondary reaction with 125I-indicator globulin (anti-rabbit or anti-human). A direct relationship between the antiserum concentration and the 125I absorption was established. Variations in titers were detectable, and the titers were comparable to complement fixation titers. Homologous and heterologous reactions were distinguishable. The method affords an objective, quantitative, and qualitative evaluation of antibody, and results are reproducible.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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