Skip to main content
PMC home page
Applied Microbiology logoLink to Applied Microbiology
. 1972 Nov;24(5):801–804. doi: 10.1128/am.24.5.801-804.1972

Quantitation of Clostridium perfringens Type A Enterotoxin by Electroimmunodiffusion

Charles L Duncan 1,2, Eileen B Somers 1,2
PMCID: PMC380666  PMID: 4344960

Abstract

Conditions for quantitation of Clostridium perfringens type A enterotoxin by electroimmunodiffusion are described. As little as 0.01 μg of enterotoxin could be detected. Electroimmunodiffusion was more sensitive than either single gel diffusion or quantitation based on erythemal activity of the toxin in guinea pig skin.

Full text

PDF
802

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Duncan C. L., Strong D. H. Ileal loop fluid accumulation and production of diarrhea in rabbits by cell-free products of Clostridium perfringens. J Bacteriol. 1969 Oct;100(1):86–94. doi: 10.1128/jb.100.1.86-94.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Hauschild A. H. Erythemal activity of the cellular enteropathogenic factor of Clostridium perfringens type A. Can J Microbiol. 1970 Aug;16(8):651–654. doi: 10.1139/m70-112. [DOI] [PubMed] [Google Scholar]
  3. Hauschild A. H., Hilsheimer R. Purification and characteristics of the enterotoxin of Clostridium perfringens type A. Can J Microbiol. 1971 Nov;17(11):1425–1433. doi: 10.1139/m71-227. [DOI] [PubMed] [Google Scholar]
  4. Hauschild A. H., Niilo L., Dorward W. J. The role of enterotoxin in Clostridium perfringens type A enteritis. Can J Microbiol. 1971 Jul;17(7):987–991. doi: 10.1139/m71-156. [DOI] [PubMed] [Google Scholar]
  5. Lopez M., Tsu T., Hyslop N. E., Jr Studies of electroimmunodiffusion: immunochemical quantitation of proteins in dilute solutions. Immunochemistry. 1969 Jul;6(4):513–526. doi: 10.1016/0019-2791(69)90191-8. [DOI] [PubMed] [Google Scholar]
  6. Miller C. A., Anderson A. W. Rapid detection and quantitative estimation of type A botulinum toxin by electroimmunodiffusion. Infect Immun. 1971 Aug;4(2):126–129. doi: 10.1128/iai.4.2.126-129.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Stark R. L., Duncan C. L. Biological characteristics of Clostridium perfringens type A enterotoxin. Infect Immun. 1971 Aug;4(2):89–96. doi: 10.1128/iai.4.2.89-96.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Weirether F. J., Lewis E. E., Rosenwald A. J., Lincoln R. E. Rapid quantitative serological assay of staphylococcal enterotoxin B. Appl Microbiol. 1966 Mar;14(2):284–291. doi: 10.1128/am.14.2.284-291.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Applied Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES